All chemicals used were analytical reagent grade and deionized–distilled water (DI water) was purified using a ZENEER UP 900 (18 MΩ/cm, human corporation, Seoul, Republic of Korea) for use throughout the experiment. Copper nitrate and L-Cysteine were purchased from Sigma-Aldrich, St. Louis, MO, USA. They were employed to synthesize the copper nanocluster. A standard stock mercury (II) solution (100 mg L−1) was prepared by dissolving 0.0135 g of mercury chloride (Quality Reagent Chemical, Auckland, New Zealand) in 100.00 mL of DI water. The working standard mercury (II) solutions were prepared by diluting the standard stock solution with DI water. A stock of o-phenylenediamine solution (100 mg L−1) was prepared by dissolving 0.0100 g of o-phenylenediamine (Alfa Aesar, Shanghai, China) in 100.00 mL of DI water. Sodium hydroxide was obtained from Carlo Erba, Milan, Italy. Sodium acetate (Sigma-Aldrich, St. Louis, MO, USA) and acetic acid (Carlo Erba, Italy) were used to prepare a buffer solution. All the glassware used was cleaned via immersion in 10% nitric acid overnight and then rinsed with DI water.
O phenylenediamine
Manufactured by Thermo Fisher Scientific
Sourced in United States, China
O-phenylenediamine is a chemical compound used in various laboratory applications. It serves as a reagent and can be used in analytical procedures, such as colorimetric detection and analysis. The core function of O-phenylenediamine is to provide a chemical reaction that can be utilized for specific experimental or testing purposes in a laboratory setting.
Automatically generated - may contain errors
Lab products found in correlation
3 3 5 5 tetramethylbenzidine (tmb), by Thermo Fisher Scientific (2 mentions)
Methanol, by Thermo Fisher Scientific (2 mentions)
Horse radish peroxidase, by Worthington (1 mentions)
Sulfuric acid, by Thermo Fisher Scientific (1 mentions)
Diammonium salt, by Thermo Fisher Scientific (1 mentions)
2 2 azino bis, by Thermo Fisher Scientific (1 mentions)
Cary 50, by Agilent Technologies (1 mentions)
Lysozyme, by Solarbio (1 mentions)
Glucose oxidase, by Avantor (1 mentions)
Horseradish peroxidase hrp, by Solarbio (1 mentions)
L ascorbic acid, by Thermo Fisher Scientific (1 mentions)
3 3 diaminobenzidine (dab), by Thermo Fisher Scientific (1 mentions)
Haucl4 3h2o, by Thermo Fisher Scientific (1 mentions)
2 2 azino bis 3 ethylbenzothiazoline 6 sulfonic acid diammoniumsalt, by J&K Scientific (1 mentions)
H2ptcl6 6h2o, by Thermo Fisher Scientific (1 mentions)
Rhodamine b, by Avantor (1 mentions)
Pbs buffer, by Solarbio (1 mentions)
α amylase, by Solarbio (1 mentions)
Trisodium citrate dihydrate, by Sinopharm (1 mentions)
Copper nitrate, by Merck Group (1 mentions)
19 protocols using o phenylenediamine
1
Colorimetric Detection of Mercury(II) Ion
2
D-Serine Oxidation Assay for DAAO
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d -Serine
was purchased
from Bachem Biosciences Inc. (King of Prussia, PA), horse radish peroxidase
from Worthington Biochemical Corporation (Freehold, NJ), and o-phenylenediamine from Pierce Biotechnology, Inc. (Rockford,
IL). All other chemicals were obtained from Sigma-Aldrich (St. Louis,
MO). A reliable 96-well plated -amino acid oxidase (DAAO)
assay was developed based on previously published methods.32 (link) Briefly,d -serine (5 mM) was oxidatively
deaminated by hDAAO in the presence of molecular oxygen and flavin
adenosine dinucleotide (FAD; 10 μM) to yield the corresponding
α-keto acid, ammonia, and hydrogen peroxide. The resulting hydrogen
peroxide was quantified using horseradish peroxidase (0.01 mg/mL)
and o-phenylenediamine (180 μg/mL), which turns
yellowish-brown upon oxidation. DAAO activity was correlated to the
rate formation of the colored product, i.e., rate of change of absorbance
at 411 nm. All reactions were carried out for 20 min at room temperature
in a 100 μL volume in Tris buffer (50 mM, pH 8.5). Additionally,
stock solutions and serial dilutions of potential DAAO inhibitors
were made in 20:80 DMSO:buffer with a final assay DMSO concentration
of 2%.
+ Expand
was purchased
from Bachem Biosciences Inc. (King of Prussia, PA), horse radish peroxidase
from Worthington Biochemical Corporation (Freehold, NJ), and o-phenylenediamine from Pierce Biotechnology, Inc. (Rockford,
IL). All other chemicals were obtained from Sigma-Aldrich (St. Louis,
MO). A reliable 96-well plate
assay was developed based on previously published methods.32 (link) Briefly,
deaminated by hDAAO in the presence of molecular oxygen and flavin
adenosine dinucleotide (FAD; 10 μM) to yield the corresponding
α-keto acid, ammonia, and hydrogen peroxide. The resulting hydrogen
peroxide was quantified using horseradish peroxidase (0.01 mg/mL)
and o-phenylenediamine (180 μg/mL), which turns
yellowish-brown upon oxidation. DAAO activity was correlated to the
rate formation of the colored product, i.e., rate of change of absorbance
at 411 nm. All reactions were carried out for 20 min at room temperature
in a 100 μL volume in Tris buffer (50 mM, pH 8.5). Additionally,
stock solutions and serial dilutions of potential DAAO inhibitors
were made in 20:80 DMSO:buffer with a final assay DMSO concentration
of 2%.
3
Quantifying D-Serine Oxidation by DAO
4
Synthesis and Characterization of Plasmonic Nanoparticles
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Cetylmethylammonium bromide (CTAB), sodium borohydride (NaBH4), chloroauric acid (HAuCl4·3H2O), silver nitrate (AgNO3), potassium tetrachloroplatinate (II) (K2PtCl4), L-ascorbic acid (AA), tetraethyl orthosilicate (TEOS), Sodium hydroxide (NaOH), sulfuric acid (H2SO4), hydrogen peroxide (H2O2), 2,2′-azino-bis(3-ethylbenzo-thiazoline-6-sulfonic acid) diammonium salt (ABTS), 3,3′,5,5′-tetramethylbenzidine (TMB) and o-phenylenediamine (OPD) were purchased from Alfa Aesar (USA). Mumps antigen was purchased from Beijing Cy-tech Biotech Co., Ltd. (China). Mumps antigen coated plate, mumps virus IgM ELISA kit were purchased from IBL International GmbH (Germany). Transmission electron microscopy (TEM) images were acquired with Tecnai G2 T20 S-TWIN microscope. Energy dispersive X-ray analysis (EDX) and scanning transmission electron microscopy (STEM) element mappings were performed on Tecnai G2 F20 U-Twin microscope. The effective diameter and zeta potential were obtained from Zetasizer Nano ZS. UV-vis-NIR absorption spectra were obtained from Perkine-Elmer Lambda 950 and Varian Cary 50. The ELISA test was performed on Infinite™ M200.
5
Synthesis and Characterization of COVID-19 Proteins
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HAuCl4·3H2O (99.9% pure), H2PtCl6·6H2O (99% pure), L-(+)-ascorbic acid (99% pure), 3,3′-diaminobenzidine (99% pure), o-phenylenediamine (98% pure) and carbamoyl-2,2,5,5-tetramethyl-3-pyrrolin-1-yloxy (CTPO, 99% pure) were provided by Alfa Aesar. 5,5-Dimethyl-1-pyrroline N-oxide (DMPO, 98% pure) and 2,2,6,6-tetramethylpiperidine (TEMP, 99% pure) were acquired from Innochem. Rhodamine B and glucose oxidase (GOx) were supplied by Amresco. Reduced cytochrome C was purchased from Abcam (Shanghai, China). 2,2′-Azinobis (3-ethylbenzo-thiazoline-6-sulfonic acid) diammonium salt (99% pure) was bought from J&K Scientific Ltd. (Beijing, China). TMB (99% pure) was obtained from Acros. Trisodium citrate dihydrate (99% pure) was provided by Sinopharm Chemical Reagent Co., Ltd. (Beijing, China). PBS buffer (pH 7.2–7.4), α-amylase, lysozyme, and horseradish peroxidase (HRP) were provided by Solarbio Science & Technology Co., Ltd. (Beijing, China). Collagen was supplied by Yuanye Co., Ltd. (Shanghai, China). SARS-CoV-2 S1 recombinant protein (S1 protein), nucleocapsid protein (N protein) and anti-SARS-CoV-2 S1 antibody were acquired from Sangon Biotech Co., Ltd. (Shanghai, China).
6
Synthesis of Benzimidazole Derivatives
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Benzil (98%) was purchased from Aldrich Chemical. o-Phenylenediamine (98%) was purchased from Alfa Aesar. Both were used without further purification.
7
Multifunctional Nanocarrier Synthesis
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Bis(2-hydroxyethyl) disulfide, 1,1,4,7,10,10-hexamethyltriethylenetetramine (HMTETA), copper (I) bromide (CuBr), iron (III) acetylacetonate (Fe(acac)3), oleic acid, tri-octylamine, DOX, (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride) (EDC), N-hydroxysuccinimide (NHS) and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) were purchased from Sigma. Amberlite® IR120, 2-bromo-2-methylpropionyl bromide, CL, rhodamine 123 (R123), GSH and pyrene were purchased from Acros. Neutral aluminium oxide (Al2O3) was purchased from Seedchem Company. Tin (Ⅱ) 2-ethylhexanoate (Sn(Oct)2) and o-phenylenediamine were purchased from Alfa Aesar. Dithiothreitol (DTT) was purchased from J.T. Baker. Tert-butyl methacrylate (tBMA) and trimethylamine (TEA) were purchased from TCI. Polyvinyl alcohol (PVA, MW=2000) was purchased from Showa. Minimum essential medium, penicillin, streptomycin and fetal bovine serum (FBS) were purchased from Biological Industries. The annexin-V/PI staining kit was purchased from Strong Biotech Corporation. Dulbecco’s modified Eagle’s medium (DMEM) was purchased from Invitrogen.
8
Quantifying Advanced Glycation Endproducts in Herbal Teas
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Herbal teas samples were purchased from a local market (Taipei, Taiwan). Glyoxal (40 % in aqueous solution), diacetyl (DA), quercetin and o-phenylenediamine were purchased from Alfa Aesar (Great Britain, UK). MethylGlyoxal (40 % in aqueous solution), sodium azide, BSA, glucose, sodium hydroxide, Folin-Ciocalteu reagent, sodium bicarbonate and lanthionine (LAN) were purchased from Sigma (St. Louis, MO, USA). Aspalathin and 3-DG were purchased from Biosynth Carbosynth (St. Gallen, Switzerland). Glucosone, 3-deoxygalactosone (3-DGal), orientin, isoorientin, vitexin, isovitexin, isoquercetin and rutin were purchased from Cayman (Ann Arbor, MI, USA). Nothofagin was purchased from Aobious (Gloucester, MA, USA). CML, CEL, MG-H1, G-H1, methylGlyoxal-lysine dimer (MOLD), Glyoxal-lysine dimer (GOLD) and furosine were purchased form Iris Biotech (Marktredwitz, Germany). Salts, sugar, canola oil, white flour and baking powder were purchased from a local supermarket (Taipei, Taiwan). All solvents used in this study are analytical or LC-MS grade and sourced from Sigma.
9
Graphene Oxide-Based Nitric Oxide Sensor
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Single layer graphene oxide (SLGO; 0.8 nm thickness, 1–5 µm diameter, 99% purity) was obtained from ACS Materials. Methanol, ascorbic acid and lead acetate were purchased from Fisher Scientific (Atlanta, GA, USA). Cerium (IV) oxide (nanoparticles dispersion, <25 nm particle size, 10 wt% in H2O) and chloroplatinic acid (8 wt%) were procured from Sigma-Aldrich (St. Louis, MO, USA). Nafion (5% aliphatic alcohol) and o-phenylenediamine were acquired from Acros organics (Newark, NJ, USA). Nitric oxide gas (CP grade 99%) was purchased from AirGas (Gainesville, FL, USA).
10
Glycation and Antioxidant Interaction Study
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