L929 murine fibroblasts

Whey protein concentrate (WPC, 85% protein content) and whey protein isolate (WPI, 90% protein content) manufactured from sweet cheese whey using cross-flow membrane filtration were purchased from Volac International Ltd. (Hertfordshire, UK). Calcium chloride, hydrogen peroxide, disodium phosphate, monosodium phosphate, 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), potassium persulphate, potassium ferricyanide, trichloroacetic acid, ferric chloride, iron sulphate, tris(hydroxymethyl)aminomethane, pyrogallol, ortophenantroline, L929 murine fibroblasts, Dulbecco’s Modified Eagle Medium (DMEM), fetal bovine serum (FBS), resazurin, l-glutamine, penicillin, streptomycin, and all other cell culture reagents were purchased from Sigma Aldrich (Darmstad, Germany). Glycerol, ammonia water, hydrochloric acid, sodium hydroxide, chloroform, ethyl acetate, ethanol and methanol were supplied from Chempur (Piekary Śląskie, Poland). Cell culture plasticware was purchased from VWR International (Radnor, PA, USA). All chemicals were of analytical grade.

To prepare the nanoplatforms, polycaprolactone (PCL, Mn 80 kDa), N,N-dimethylformamide (DMF), chloroform (99%), N,N-isopropylacrylamide (NIPAAm, 97%), N-isopropylomethacrylamide (NIPMAAm, 97%), N,N′-methylene bisacrylamide (BIS-AAm, 99.5%), 2-hydroxy-4′-(2-hydroxyethoxy)-2-methylpropiophenone (Irgacure 2959, 98%), ammonium persulfate (APS, 98%), N,N,N′,N′-tetramethylethylenediamine (TEMED, 99%) were bought from Sigma-Aldrich (Poland). Gold nanorods (AuNRs, λ = 800 nm, OD = 50, C = 0.88 mg mL⁻¹) from nanoComposix (USA) were used as received.
To perform biocompatibility tests, L929 murine fibroblasts, bovine serum albumin (BSA), phosphate buffer saline (PBS), Triton X, and DAPI were obtained from Sigma-Aldrich (Poland). Dulbecco's modified Eagle's medium (DMEM), fetal bovine serum (FBS), penicillin-streptomycin (PS), and EDTA-trypsin were bought from Gibco Invitrogen (USA). Alexa Fluor 488 Phalloidin, PrestoBlue reagent and live/dead cytotoxicity kit assay were purchased from Thermo-Fisher Scientific (USA).