10 000 MDA-MB-231 CD73 +and CD73- cells were seeded per wells of a 96-well plate and cultured in assay media complemented with 2 mM glutamine (Sigma), 25 mM glucose (Sigma) and 10% FBS for 3 days. Supernatant was collected daily and stored at –20 °C until analysis. glutamine levels in media was measured using a commercially available kit (Promega) according to manufacturer’s protocol.
Glutamine
Manufactured by Promega
Glutamine is a laboratory product that functions as an amino acid. It is a natural component found in the human body and is essential for various cellular processes.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using glutamine
1
Glutamine Consumption in Breast Cancer
2
Glutamine Consumption in Breast Cancer
Check if the same lab product or an alternative is used in the 5 most similar protocols
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10 000 MDA-MB-231 CD73 +and CD73- cells were seeded per wells of a 96-well plate and cultured in assay media complemented with 2 mM glutamine (Sigma), 25 mM glucose (Sigma) and 10% FBS for 3 days. Supernatant was collected daily and stored at –20 °C until analysis. glutamine levels in media was measured using a commercially available kit (Promega) according to manufacturer’s protocol.
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