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N 3 oxo octanoyl l homoserine lactone

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N-3-oxo-octanoyl-L-homoserine lactone is a chemical compound used in laboratory research. It functions as a quorum sensing molecule, which is a signaling mechanism used by bacteria to coordinate their behavior in response to cell population density. This compound is utilized by researchers to study bacterial communication and social interactions.

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Lab products found in correlation

N hexanoyl l homoserine lactone, by Merck Group (4 mentions) N 3 oxo decanoyl l homoserine lactone, by Merck Group (4 mentions) N 3 oxo dodecanoyl l homoserine lactone, by Merck Group (3 mentions) N butyryl dl homoserine lactone, by Merck Group (3 mentions) N 3 oxo hexanoyl l homoserine lactone, by Merck Group (3 mentions) N octanoyl l homoserine lactone, by Merck Group (3 mentions) N decanoyl dl homoserine lactone, by Merck Group (2 mentions) N dodecanoyl dl homoserine lactone, by Merck Group (2 mentions) N 3 hydroxy dodecanoyl dl homoserine lactone, by Merck Group (2 mentions) Formic acid for hplc, by Merck Group (1 mentions) Acetonitrile, by Merck Group (1 mentions) N acylhomoserine lactones, by Merck Group (1 mentions) Formic acid, by Merck Group (1 mentions) Hplc grade acetonitrile, by Merck Group (1 mentions) Paraoxon, by Merck Group (1 mentions) P nitrophenol, by Merck Group (1 mentions) Methyl parathion, by Merck Group (1 mentions) Diazinon, by Merck Group (1 mentions) Malathion, by Merck Group (1 mentions) Dimethoate, by Merck Group (1 mentions)

5 protocols using n 3 oxo octanoyl l homoserine lactone

1

AHL Compound Standards for Quorum Sensing

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The following AHL standards, purchased from University of Nottingham, UK were: N-3-oxo-butyryl-L-homoserine lactone (3OC4-HSL), N-3-hydroxy-butyryl-L-homoserine lactone (3OHC4-HSL), N-3-hydroxy-hexanoyl-L-homoserine lactone (3OHC6-HSL), N-3-hydroxy-octanoyl-L-homoserine lactone (3OHC8-HSL), N-3-hydroxy-decanoyl-L-homoserine lactone (3OHC10-HSL). In addition, N-butyryl-DL-homoserine lactone (C4-HSL), N-hexanoyl-L-homoserine lactone (C6-HSL), N-3-oxo-hexanoyl-L-homoserine lactone (3OC6-HSL), N-octanoyl-L-homoserine lactone (C8-HSL), N-3-oxo-octanoyl-L-homoserine lactone (3OC8-HSL), N-decanoyl-DL-homoserine lactone (C10-HSL), N-3-oxo-decanoyl-L-homoserine lactone (3OC10-HSL), N-dodecanoyl-DL-homoserine lactone (C12-HSL), N-3-oxo-dodecanoyl-L-homoserine lactone (3OC12-HSL), N-3-hydroxy-dodecanoyl-DL-homoserine lactone (3OHC12-HSL), acetonitrile, and formic acid for HPLC were obtained from Sigma-Aldrich (St. Louis, MO, USA).
Khider M., Hansen H., Hjerde E., Johansen J.A, & Willassen N.P. (2019). Exploring the transcriptome of luxI− and ΔainS mutants and the impact of N-3-oxo-hexanoyl-L- and N-3-hydroxy-decanoyl-L-homoserine lactones on biofilm formation in Aliivibrio salmonicida. PeerJ, 7, e6845.
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2

Marine Bacteria Quorum Sensing Inhibition

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The marine bacterial strains analysed in this study are summarized in Table 2. Marine agar or broth (MA or MB respectively) was routinely used to grow marine bacterial strains (Difco, Oxford, UK). SYP agar with 1.5% of sea salt and MA or MB were used in different experiments performed in this work to analyse the potential QSI capability of marine bacteria. All marine bacterial strains used in this study were grown routinely at 23°C. Different N‐Acyl homoserine lactones (AHLs) used in this work were purchased from Sigma‐Aldrich (Cambridge, UK) and were dissolved in DMSO (N‐butyryl‐dl‐homoserine lactone: C4‐HSL; N‐hexanoyl‐l‐homoserine lactone: C6‐HSL; N‐(3‐oxooctanoyl)‐l‐homoserine lactone: 3OC8‐HSL; N‐(3‐oxodecanoyl)‐l‐homoserine lactone: 3OC10‐HSL; and N‐(3‐oxododecanoyl)‐l‐homoserine lactone: 3OC12‐HSL).
Gutiérrez‐Barranquero J.A., Reen F.J., Parages M.L., McCarthy R., Dobson A.D, & O'Gara F. (2017). Disruption of N‐acyl‐homoserine lactone‐specific signalling and virulence in clinical pathogens by marine sponge bacteria. Microbial Biotechnology, 12(5), 1049-1063.
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3

Analytical Standards for AHL Quorum Sensing

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The following AHL standards were purchased from University of Nottingham, UK: N-3-oxo-butyryl-L-homoserine lactone (3-oxo-C4-HSL), N-3-hydroxy-butyryl-L-homoserine lactone (3-OH-C4-HSL), N-3-hydroxy-hexanoyl-L-homoserine lactone (3-OH-C6-HSL), N-3-hydroxy-octanoyl-L-homoserine lactone (3-OH-C8-HSL), N-3-hydroxy-decanoyl-L-homoserine lactone (3-OH-C10-HSL). Standards purchased from Sigma-Aldrich were: N-butyryl-DL-homoserine lactone (C4-HSL), N-hexanoyl-L-homoserine lactone (C6-HSL), N-3-oxo-hexanoyl-L- homoserine lactone (3-oxo-C6-HSL), N-octanoyl-L-homoserine lactone (C8-HSL), N-3-oxo-octanoyl-L-homoserine lactone (3-oxo-C8-HSL), N-decanoyl-DL-homoserine lactone (C10-HSL), N-3-oxo-decanoyl-L-homoserine lactone (3-oxo-C10-HSL), N-dodecanoyl-DL-homoserine lactone (C12-HSL), N-3-oxo-dodecanoyl-L-homoserine lactone (3-oxo-C12-HSL), and N-3-hydroxy-dodecanoyl-DL-homoserine lactone (3-OH-C12-HSL). HPLC grade acetonitrile and formic acid were purchased from Merck.
Hansen H., Purohit A.A., Leiros H.K., Johansen J.A., Kellermann S.J., Bjelland A.M, & Willassen N.P. (2015). The autoinducer synthases LuxI and AinS are responsible for temperature-dependent AHL production in the fish pathogen Aliivibrio salmonicida. BMC Microbiology, 15, 69.
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4

Pesticide and Quorum Sensing Compound Acquisition

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Parathion, methyl parathion, diazinon, chlorpyrifos, malathion, dimethoate, and demeton-S-methyl were purchased from Supelco (Bellefonte, PA, USA). Poly(vinyl alcohol) 16/1 (84 kDa) was purchased from Sinopec Corp. (Beijing, China). Paraoxon, p-nitrophenol, N-homoserine lactone hydrochloride, N-butyryl-D,L-homoserine lactone, N-(3-oxooctanoyl)-L-homoserine lactone, N-(3-oxodecanoyl)-L-homoserine lactone, N-(3-oxododecanoyl)-L-homoserine, and other chemicals were acquired from Sigma-Aldrich (St. Louis, MO, USA).
Efremenko E., Stepanov N., Maslova O., Senko O., Aslanli A, & Lyagin I. (2022). “Unity and Struggle of Opposites” as a Basis for the Functioning of Synthetic Bacterial Immobilized Consortium That Continuously Degrades Organophosphorus Pesticides. Microorganisms, 10(7), 1394.
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5

Bacterial Quorum Sensing Signaling Molecules

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N-Hexanoyl-L-homoserine lactone (C6-HSL), N-octanoyl-L-homoserine lactone (C8-HSL), N-decanoyl-L-homoserine lactone (C10-HSL), N-dodecanoyl-L-homoserine lactone (C12-HSL), N-(3-oxo-hexanoyl)-L-homoserine lactone (3-oxo-C6-HSL), N-(3-oxo-octanoyl)-L-homoserine lactone (3-oxo-C8-HSL), and HSL standards were purchased from Sigma. N-(3-oxo-decanoyl)-L-homoserine lactone (3-oxo-C10-HSL), N-(3-oxo-dodecanoyl)-L-homoserine lactone (3-oxo-C12-HSL), and N-(3-oxo-tetradecanoyl)-L-homoserine lactone (3-oxo-C14-HSL) were obtained from the Nottingham University in England.
Kusada H., Zhang Y., Tamaki H., Kimura N, & Kamagata Y. (2019). Novel N-Acyl Homoserine Lactone-Degrading Bacteria Isolated From Penicillin-Contaminated Environments and Their Quorum-Quenching Activities. Frontiers in Microbiology, 10, 455.
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