Trans 2 hexen 1 ol

Analytical-grade chemicals and reagents for preparing standard solutions for GC-MS analysis were used without further purification. Standard 1-octen-3-one (96%), 1-octen-3-ol (≥98%), 3-octanone (≥98%), benzaldehyde (≥99.5%), trans-2-hexen-1-ol (96%), benzyl alcohol (99%+), 1,5-dimethyl naphthalene (98%), 3-methyl-1-butanol (99%+), 1,5-dimethyltetralin (≥90%), phenylethyl alcohol (≥99%), 4-methyl-2-pentanol (98%), glucose (≥99.5%), fructose (≥99%), tartaric acid (99%), naphthalene-d8 (99 atom% D, ≥98% (CP)), 3-octanol (99%), 2-octanol (≥99.5%), zinc nitrate hexahydrate (98%), 2-methylimidazole (98%), and glass wool were purchased from Sigma-Aldrich (Sydney, NSW, Australia). Hexanal (98%), 1-hexanol (99%), menthol (HPLC), and N,N-dimethylformamide (DMF) (99%) were purchased from Alfa Aesar (Scoresby, VIC, Australia). Hexanal-d12 (98.5 atom % D) and 2,4,6-tribromoanisol-d5 (99 atom % D) were purchased from CDN Isotopes (Pointe-Claire, Quebec, Canada). Milli-Q™ water (18 MΩ·cm) was collected through a Millipore™ water purification system (Merk, Bayswater, VIC, Australia). Synthetical grape juice was made using 100 g glucose, 100 g fructose, and 4 g tartaric acid. All solutions were prepared in Milli-Q™ water, with the pH adjusted to 7.0.

Hexanal, trans-2-hexen-1-ol, 1-octen-3-ol, α-lipoic acid, and solvents were purchased from Sigma-Aldrich. Sepharose 4 Fast Flow resin for pOBP purification was acquired from GE Healthcare. DEAE–Sepharose for glutamine-binding protein (GlnBP) purification was obtained from Sigma-Aldrich. Thin-film gold single electrodes (ED-SE1-Au) were purchased from Micrux Technologies (Oviedo, Spain). Materials used for protein electrophoresis were obtained from Bio-Rad. All the used reagents were at analytical or higher grades available. All the solutions were prepared using Milli-Q water.