Rab14

Manufactured by Santa Cruz Biotechnology

Sourced in United States

RAB14 is a member of the Rab family of small GTPases. It functions as a regulatory protein involved in intracellular membrane trafficking and vesicle transport.

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Lab products found in correlation

2 protocols using rab14

Comprehensive Antibody Panel for Cellular Analysis

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The following primary antibodies were used: MLKL (Cell Signaling 14993), GAPDH (Santa Cruz sc-32233), CD9 (System Biosciences EXOAB-CD9A-1), GM130 (Abcam ab52649), Steap3 (Abcam ab151566), RAB14 (Santa Cruz sc-271401), M6PR for WB (Cell Signaling 14364), M6PR for immunofluorescence (Abcam ab2733), CD63 for WB (System Biosciences EXOAB-CD63A-1), CD63 for immunofluorescence (BD Biosciences 556019), SOX2 (Millipore AB5603), NESTIN (Millipore MAB5326), LAMP2 (Santa Cruz sc-18822), phospho-Histone H3 (Cell Signaling 3377), caspase-3 (Cell Signaling 9662), caspase-8 (Cell Signaling 9746), PARP (Santa Cruz sc-8007), GSDME (Abcam ab215191), phospho-ATM (Cell Signaling 5883), phospho-p65 (Cell Signaling 3033), P65 (Santa Cruz sc-372), phospho-IκBα (Cell Signaling 9246), IκBα (Cell Signaling 4814), RIPK1 (BD Biosciences 551042), RIPK3 (Bethyl 13526), TUBULIN (Santa Cruz sc-8035), phospho-MLKL S358 (Abcam 187091), ALIX (BioLegend 634502), EEA1 (BD Biosciences 610456), RAB27A (Cell Signaling 95394), cleaved-caspase 3 (Cell Signaling 9664), and eGFP (Millipore AB10145). HRP-conjugated antibodies (1/5000) were from Southern Biotech, and Alexa-conjugated antibodies (1/200) from Life Technologies.

André-Grégoire G., Maghe C., Douanne T., Rosińska S., Spinelli F., Thys A., Trillet K., Jacobs K.A., Ballu C., Dupont A., Lyne A.M., Cavalli F.M., Busnelli I., Hyenne V., Goetz J.G., Bidère N, & Gavard J. (2022). Inhibition of the pseudokinase MLKL alters extracellular vesicle release and reduces tumor growth in glioblastoma. iScience, 25(10), 105118.

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Characterizing CCN2 and Rab14 Expression

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The lysate of HCS-2/8, COS7, and MDA-MB-231 cells was analyzed by Western Blotting with an antibody against CCN2 (R&D Systems, Minneapolis, MN, USA), Rab14 (Santa Cruz, Dallas, TX, USA), and β-Actin (Sigma, St Louis, MO, USA), respectively. The COS7 cells that were transfected with pEGFP⁄ccn2 or pFlag-CMV⁄Rab14-HALO (wt or mutants) were incubated for 48 h. The lysates were harvested and analyzed by Western Blotting with an antibody against GFP or an antibody against Rab14. Western Blot analysis was performed as described previously [5 (link)].

Hoshijima M., Hattori T., Aoyama E., Nishida T., Kubota S., Kamioka H, & Takigawa M. (2020). Roles of Interaction between CCN2 and Rab14 in Aggrecan Production by Chondrocytes. International Journal of Molecular Sciences, 21(8), 2769.

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