Shim pack clc ods c18 column

The saturated equilibrium solubility of MCA was examined in diverse oil phases (Labrafac PG, Peceol, Maisine CC, Capryol 90, Labrafil M 1944 CS, Labrafac Lipophile WL 1349, Capryol 90, ethyl oleate and oleic acid), surfactants (Kolliphor RH40, Kolliphor HS15, Kolliphor ELP, Tween 80, Labrasol and Lauroglycol FCC), and co-surfactants (1,2-propanediol, PEG 400, and Transcutol HP). An excess amount of MCA was added to 0.5 mL of different oil phases, surfactants and co-surfactants, the mixture was vortexed for 5 min, ultrasound treatment for 30 min, and placed in a shaker at room temperature for 48 h. Then, the equilibrated samples were centrifuged at 14,000 rpm for 10 min, each supernatant was collected, diluted with methanol, passed through 0.22 μm membrane filter, and analyzed by Agilent 1260 HPLC system (Agilent Technologies, Palo Alto, CA, USA). Chromatographic separation was performed on a Shim-pack CLC-ODS C₁₈ column (4.6 mm × 150 mm, particle size 5 μm, Shimadzu, Kyoto, Japan). The mobile phase consisted of acetonitrile and 0.1% formic acid aqueous solution (70:30, v/v) at flow rate 1.0 mL/min. The column compartment was set at 40°C and detective wavelength was 204 nm. The examination was performed in triplicate and the standard deviation (SD) was calculated.