Titanium 4 oxysulfate solution

For the generation of oxidants,
deionized water (Millipore Mili-Q system, resistivity 18.2 MΩ·cm
at 25 °C, TOC:2 ppb) and the following analytical standard reagents
were used: sodium chloride, sodium perchlorate, 70% perchloric acid,
and 1.9–2.1% of titanium(IV) oxysulfate solution (CAS:13825–74–6/Sigma-Aldrich)
as an indicator for H₂O₂ measurement and oxidants
analysis, and the samples were acidified with 20% sulfuric acid for
subsequent reaction with sodium thiosulfate. For the effluent preparation,
16.21 mg L^–1 methomyl from its commercial compound
was used, whose composition is 215 g L^–1 methomyl,
420 g L^–1 ethanol, and 324 g L^–1 other ingredients.

Mitoxantrone (98%) was purchased from Bosche Scientific (New Brunswick, NJ, USA). Tetraethyl orthosilicate (TEOS, 98%), hexadecyltrimethylammonium bromide (CTAB ≥ 98%), dichloromethane anhydrous (DCM, ≥ 99.8%), iron (II) chloride tetrahydrate (FeCl₂·4H₂O, 98%), iron (III) chloride hexahydrate (FeCl₃·6H₂O, 97%), titanium (IV) oxysulfate solution (TiOSO₄, 1.9 − 2.1%), hydrogen peroxide (H₂O₂, 32%), glacial acetic acid (≥ 99%), ammonium hydroxide solution (NH₄OH, 30–33%), 3-(triethoxysilyl)propyl isocyanate (95%), human serum (HS), dimethyl sulfoxide (DMSO), Dulbecco’s Modified Eagle’s Medium (DMEM) (with 4,5 g L^− 1 of glucose, stable glutamine and sodium bicarbonate), trypsin (1X), penicillin-streptomycin, L-glutamine, non-essential aminoacids, phosphate buffered saline (PBS) and Thiazolyl Blue Tetrazolium Bromide (MTT) were obtained from Sigma-Aldrich (Madrid, Spain). Absolute ethanol (EtOH), petroleum ether, sodium hydroxide and hydrochloric acid (35% w/w) were bought from Scharlab (Barcelona, Spain). Triton X-100 was purchased from PanReac AppliChem (Barcelona, Spain). DAPI (4′,6-diamidino-2-phenylindole) and Alexa Fluor 647 Conjugated Wheat Germ Agglutinin (WGA) were obtained from Fisher Scientific (Madrid, Spain).

The following reagents
were used to perform the experiments: sodium sulfate (PanReac AppliChem),
sulfuric acid (Scharlab), 60% w/w poly(tetrafluoroethylene) dispersion
(PTFE—Uniflon), and titanium(IV) oxysulfate solution (Sigma-Aldrich).
The aqueous solutions were prepared using ultrapure water (Milli-Q
system with resistivity > 18 MΩ cm). Printex L6 carbon (PL6C)
was purchased from Evonik Ltd. (Brazil).