Total cells present in muscle after FAE injury were isolated by enzymatic digest as previously described.10 (link) Cells were washed with 1% bovine serum albumin in phosphate-buffered saline (PBS); blocked with 2% bovine serum albumin in PBS with addition of Fcγ-block (eBioscience, San Diego, Calif); and stained with either a cocktail containing phycoerythrin-conjugated anti-CD11b (BD Biosciences, Franklin Lakes, NJ), peridinin-chlorophyll-conjugated anti-Gr1 (BioLegend, San Diego, Calif), fluorescein isothiocyanate-conjugated anti-Ly6C (BioLegend), and allophycocyanin-conjugated anti-F4/80 (BioLegend) or a cocktail of the corresponding isotype controls. Samples were run on the BD Fortessa Flow Cytometer (BD Biosciences) at The University of Texas at Austin Institute of Cell and Molecular Biology core facility with the forward scatter settings gated to exclude red blood cell-sized cells. Data were analyzed using FlowJo software (FlowJo, LLC, Ashland, Ore).
Phycoerythrin conjugated anti cd11b
Manufactured by BD
Sourced in United States
Phycoerythrin-conjugated anti-CD11b is a fluorescently labeled antibody that binds to the CD11b cell surface marker. CD11b is expressed on the surface of certain immune cells, including monocytes, macrophages, and granulocytes. The phycoerythrin fluorescent dye is conjugated to the anti-CD11b antibody, allowing for the detection and analysis of CD11b-positive cells using flow cytometry or other fluorescence-based techniques.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using phycoerythrin conjugated anti cd11b
1
Flow Cytometric Analysis of FAE-Induced Muscle Cells
2
Isolation and Analysis of Liver-Infiltrating Leukocytes
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Liver-infiltrating leukocytes were isolated by isotonic Percoll centrifugation (900g, 25 minutes). Red blood cells were lysed using ACT lysis buffer (NH4Cl, 140 mM; C4H11NO3, 17 mM; pH 7.2). The leukocytes were resuspended in fluorescence-activated cell sorter (FACS) medium (1 × PBS, 2 mM EDTA, 0.5% BSA, 0.09% sodium azide). Then, 1 × 106 cells of each sample were stained using the following antibodies: FITC-conjugated anti-NK1.1, allophycocyanin (APC)-conjugated anti-Gr-1 and phycoerythrin-conjugated anti-CD11b were from BD Pharmingen (San Diego, CA, USA). Phycoerythrin-Cy5-conjugated anti-CD3 was purchased from Tonbo Biosciences (San Diego, CA, USA). After staining procedures, cells were washed twice with FACS medium, and analyzed on the Attune NxT acoustic focusing cytometer (Applied Biosystems; Life Technologies, Waltham, MA, USA). The results were analyzed using FlowJo v10 software (FlowJo, LLC, Ashland, OR, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!