Anti pdgfrb

Manufactured by Santa Cruz Biotechnology

Sourced in United States

Anti-PDGFRb is a laboratory reagent used for the detection and analysis of the Platelet-Derived Growth Factor Receptor Beta (PDGFRB) protein. It is a specific antibody that binds to PDGFRB, allowing researchers to measure its expression or localization in biological samples.

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Lab products found in correlation

Anti human calponin, by Abcam (2 mentions) Anti human ng2, by R&D Systems (2 mentions) Texas red anti mouse igg, by Vector Laboratories (2 mentions) Hiperfect transfection reagent, by Qiagen (1 mentions) Anti actin clone c4, by Merck Group (1 mentions) Imatinib, by Cayman Chemical (1 mentions) Anti pdgfra, by Santa Cruz Biotechnology (1 mentions) Anti flt3, by Santa Cruz Biotechnology (1 mentions) Sirnas, by Qiagen (1 mentions) Sybr premix ex taq 2, by Takara Bio (1 mentions) Dnase 1, by Merck Group (1 mentions) 4 6 diamidine 2 phenylindole dihydrochloride dapi, by Merck Group (1 mentions) Ab3594, by Merck Group (1 mentions) Cy5 conjugated donkey anti rabbit, by Jackson ImmunoResearch (1 mentions) Rnaiso plus 9109, by Takara Bio (1 mentions) Primescrip rt master mix, by Takara Bio (1 mentions) Cy3 conjugated donkey anti rabbit, by Jackson ImmunoResearch (1 mentions) β actin, by MultiSciences Biotech (1 mentions) Cell tak, by Thermo Fisher Scientific (1 mentions) Vectashield with dapi, by Vector Laboratories (1 mentions)

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PDGFRB (4 citations)

6 protocols using anti pdgfrb

Kinase Inhibitor Targeting in Cells

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Imatinib (Cayman Chemical, Michigan, USA) and Crenolanib (Selleckman) were dissolved in DMSO to give a final concentration of 10 mM, and used at the previously established IC₃₀ concentration, that is of 25 μM and 1 μM respectively. The following primary antibodies were used: primary mouse monoclonal anti-PDGFRB, anti-PDGFRA, and anti-FLT3 (Santa Cruz, TX, USA; 1:300) or mouse monoclonal anti-ß-actin (Anti-Actin, Clone C4, Millipore, MA, USA; 1:5000). IgG-HRP Santa Cruz (1:5000) was used as secondary antibody. Different silencing-RNAs (siRNAs) were tested, and purchased from Qiagen (Qiagen, UK). Further experiments were performed by using a single specific siRNA for PDGFRB (SI00605738, the so-called “siPDGFRB” now-on) and the “AllStars Negative Control siRNA” (SI03650318, used as non-targeting control - the so-called “C-PDGFRB” now-on). siRNA oligonucleotides were re-suspended in the provided buffer at a final stock concentration of 20 mM, and employed at 50nM in each experiments. HiPerfect transfection reagent was employed for siRNA trasfection (Qiagen, UK), as previously described [16 (link)].

Melaiu O., Catalano C., De Santi C., Cipollini M., Figlioli G., Pellè L., Barone E., Evangelista M., Guazzelli A., Boldrini L., Sensi E., Bonotti A., Foddis R., Cristaudo A., Mutti L., Fontanini G., Gemignani F, & Landi S. (2017). Inhibition of the platelet-derived growth factor receptor beta (PDGFRB) using gene silencing, crenolanib besylate, or imatinib mesylate hampers the malignant phenotype of mesothelioma cell lines. Genes & Cancer, 8(1-2), 438-452.

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Fluorescent Markers for Neurodegeneration

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Fluorescein‐conjugated dextran (D3306, molecular weight: 3 kDa, Dex‐3) was purchased from Invitrogen. Gd‐DPTA (molecular weight: 938 Da) was obtained from CONSUN. HiLyte Fluor‐488‐labeled recombinant human α‐syn (AS‐55457, molecular weight: 14 kDa HiLyte‐488‐α‐syn) was purchased from Anaspec. Adeno‐associated virus expressing A53T‐α‐syn (AAV‐A53T) was designed by OBio Technology. Primary antibody anti‐AQP4 (rabbit, AB3594), anti‐α‐syn (mouse, 36–008; Syn211), and anti‐tyrosine hydroxylase (rabbit, AB152; anti‐TH) were purchased from Millipore. Anti‐PDGF‐B (rabbit, DF6328) was purchased from Affinity Biosciences, and anti‐PDGFRb (mouse, sc‐374573) was purchased from Santa Cruz Biotechnology. β‐actin (mouse, ab008‐100), horseradish peroxidase‐conjugated anti‐rabbit and anti‐mouse IgG (70‐gam007, 70‐gar007) were purchased from Multisciences. Secondary antibodies, including Cy3‐conjugated donkey anti‐rabbit and Cy5‐conjugated donkey anti‐rabbit, were purchased from Jackson ImmunoResearch. DNaseI and 4′,6‐diamidine‐2′‐phenylindole dihydrochloride (DAPI) were purchased from Sigma‐Aldrich. RNAiso Plus (9109), PrimeScrip RT Master Mix (RR036A) and SYBR Premix Ex Taq II (RR820A) were purchased from TaKaRa.

Song J., Li Z., Xue X., Meng J., Zhu W., Hu S., Xu G, & Wang L. (2024). Neonatal stress disrupts the glymphatic system development and increases the susceptibility to Parkinson's disease in later life. CNS Neuroscience & Therapeutics, 30(2), e14587.

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Immunohistochemical Staining of Mouse Cochlear Stria

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Mice were perfused transcardially with PBS followed by 4% paraformaldehyde. Cochleae were dissected and fixed in paraformaldehyde overnight. Cochleae were washed in PBS for 15 minutes, the stria dissected, and mounted on slides pre-coated with Cell-Tak (Fisher) into a drop of PBS. The PBS is aspirated away to allow the stria to adhere to the slide, and then permeabilized using 0.5% Triton X-100 in 1X PBS for 30 minutes. A pre-block of 1% fish gelatin in PBS is applied for 1 hour, then primary antibodies added and incubated overnight in 1% BSA and 1X PBS. We used anti-desmin (1:50, abcam, cat#ab32362) and fluorescence-labeled isolectin GS-IB4 (1:300, Invitrogen, cat#121411, Alexa fluor 488), or anti-PDGFRb (1:100, Santa Cruz, Cat # sc-6252, Lot # G149). The secondary antibody for anti-desmin was anti-rabbit at 1:400 or for PDGFRb, anti-mouse at 1:400. The next day the slides were washed 3X for 10 minutes each with PBS and incubated with the secondary antibody for 1 hour at room temperature and mounted using Prolong gold mounting medium.

Dufek B., Meehan D.T., Delimont D., Samuelson G., Madison J., Shi X., Boettcher F., Gratton M.A., Trosky V, & Cosgrove D. (2020). Pericyte Abnormalities Precede Strial Capillary Basement Membrane Thickening in Alport Mice. Hearing research, 390, 107935.

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Co-culture of ECFC and bmMPC Differentiates into VSMC/Pericytes

Cited 1 time

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Culture chamber slides were coated with fibronectin and seeded with ECFC and bmMPC at a 1:1 ratio. After in vitro co-culture for 7 days, bmMPC differentiate into VSMC/pericytes [43 (link)]. Once differentiated, cells were fixed with cold pure methanol on ice for 10 min. For immunostaining of ECFC, samples were incubated with a mAb anti-human von Willebrand factor (Dako) for 1 h at room temperature, followed by incubation with the secondary antibody Texas Red anti-mouse IgG (Vector) for 1 h at room temperature. Differentiated bmMPCs were incubated with anti-human calponin (Abcam), anti-human Sm22α (Abcam), anti-PDGFRb (Santa Cruz), anti-human NG2 (R&D Systems), anti-human αSMA (Sigma) or a negative control antibody (Santa Cruz). After washing samples were incubated with the appropriate FITC-labeled secondary antibody, and mounted using Vectashield with DAPI (Vector).

Rossi E., Smadja D.M., Boscolo E., Langa C., Arevalo M.A., Pericacho M., Gamella-Pozuelo L., Kauskot A., Botella L.M., Gaussem P., Bischoff J., Lopez-Novoa J.M, & Bernabeu C. (2015). Endoglin regulates mural cell adhesion in the circulatory system. Cellular and Molecular Life Sciences, 73, 1715-1739.

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Aortic Valve and Artery Morphology Analysis

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For morphological analysis, sections of aortic valves or brachiocephalic arteries were stained with hematoxylin and eosin (H&E) or Masson’s trichrome stain. Immunofluorescence staining was performed by incubation with rabbit anti-CD45 (Abcam,1:100), anti-ICAM-1 (Biolegend, 1:100), anti-PDGFRb (Santa Cruz, 1:50) followed by FITC-anti-rabbit or rat IgG. FITC-anti-SMA antibody (Sigma) staining was used for tdTomato tracing immunofluorescent assays. Details of antibodies can be found in the Major Resources Table. In situ RNAscope assays for Pdgfrb, Sca1, KLF4, and Spry1 were performed according to RNAscope fluorescent multiplex assay instructions (ACDBio). Images were captured using Canon EOS camera and remote imaging software (Canon), or a Leica SP8 confocal microscope. Quantification was performed using ImageJ.

Zhu Y., Traub L.M, & Kornfeld S. (1998). ADP-Ribosylation Factor 1 Transiently Activates High-Affinity Adaptor Protein Complex AP-1 Binding Sites On Golgi Membranes. Molecular Biology of the Cell, 9(6), 1323-1337.

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Immunostaining of Differentiated Perivascular Cells

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Culture chamber slides were coated with gelatin and seeded with ECFCs control-siRNA+ MSCs or with ECFCs endoglin-siRNA+ MSCs at a 1:1 ratio. After co-culture for 7 days, MSCs differentiate into perivascular cells (11) . Once differentiated, the cells were fixed with cold pure methanol on ice for 10 min. For ECFC immunostaining, samples were incubated with a mouse antibody against human von Willebrand factor (Dako) for 1 h at room temperature, followed by the secondary antibody Texas Red anti-mouse IgG (Vector) for 1 h at RT. Differentiated MSCs were incubated with anti-human calponin (Abcam), anti-PDGFRb (Santa Cruz), anti-human NG2 (R&D Systems), anti-human aSMA (Sigma) or a negative control antibody (Santa Cruz). After washing, samples were incubated with the appropriate FITC-labeled secondary antibody, nuclei were counterstained with TO-PRO-3 (642/661, Invitrogen) and samples were mounted in IBDI mounting medium.

Rossi E., Smadja D., Goyard C., Cras A., Dizier B., Bacha N., Lokajczyk A., Guerin C.L., Gendron N., Planquette B., Mignon V., Bernabéu C., Sanchez O, & Smadja D.M. (2017). Co-injection of mesenchymal stem cells with endothelial progenitor cells accelerates muscle recovery in hind limb ischemia through an endoglin-dependent mechanism. Thrombosis and haemostasis, 117(10).

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