Na2hpo4 2h2o

Alpha-amylase, CaCl₂ ∙ 2H₂O, 4′,6′-diamidino-2-phenylindole (DAPI), 2′,7′-dichlorofluorescin-diacetate (DCFH-DA), MgCl₂ ∙ 6H₂O, mucin, ox bile, pancreatin, paraformaldehyde, pepsin, trypsin and ZnO nanopowder (#677450 and #544906) were purchased from Sigma-Aldrich Chemie GmbH, Taufkirchen, Germany. Dulbecco’s Modified Eagle Medium (DMEM), foetal bovine serum (FBS), non-essential amino acids, penicillin/streptomycin, and trypsin/EDTA were obtained from PAN-Biotech GmbH, Aidenbach, Germany. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), dimethyl sulfoxide, fluorescein isothiocyanate (FITC)-dextran, hydrogen peroxide (30%), nitric acid (Suprapur), and triton X-100 were acquired from Merck KGaA, Darmstadt, Germany. 2-((3-Chlorophenyl) hydrazinylidene) propanedinitrile (CCCP) and ZnCl₂ were procured from Thermo Fisher Scientific Inc., Waltham, MA, USA. Carbamide, ethylene glycol tetraacetic acid (EGTA), KCl, KH₂PO₄, NaCl, NaHCO₃, and Na₂HPO₄ ∙ 2H₂O were purchased from Carl Roth GmbH & Co. KG, Karlsruhe, Germany. Cacodylic acid and glutaraldehyde were bought from Serva Electrophoresis GmbH, Heidelberg, Germany. 5,5′,6,6′-Tetrachloro-1,1′,3,3′-tetraethylbenzimidazolylcarbocyanine iodide (JC-1) was obtained from Enzo Life Sciences GmbH, Lörrach, Germany. Phalloidin-iFlour 488 reagent was acquired from Abcam plc., Cambridge, UK.

Cell culture flasks and 96-well plates were purchased from Sarstedt (Nürnbrecht, Germany). Cell culture media (Dulbecco’s Minimal Essential Medium (DMEM) and Eagle’s Minimal Essential Medium (EMEM) without phenol red) and supplements (fetal bovine serum (FBS), charcoal–dextran stripped FBS (CD-FBS), blasticidin S HCl and geneticin (G418)) were produced from Gibco and obtained from Thermo Fisher Scientific (Waltham/MA, USA). ZEN, α-ZEL, E2, (Z)-4-hydroxytamoxifen (4-OH-TAM) and sulforhodamine B (SRB) were purchased from Sigma-Aldrich Chemie GmbH (Schnelldorf, Germany). DAI, EQ, and GEN were obtained from Extrasynthese (Genay Cedex, France) whereas dimethly sulfoxide (DMSO), NaCl, KCl, Na₂HPO₄, Na₂HPO₄ * 2 H₂O, and KH₂PO₄ were purchased from Carl Roth GmbH + Co. KG (Karlsruhe, Germany). The CellTiter-Blue^® Cell Viability Assay Kit and ONE-Glo™ EX luciferase assay system were obtained from Promega Corporation (Madison/WI, USA).

Cell culture 96-well plates and flasks were obtained from Sarstedt (Nürnbrecht, Germany). Cell culture media (Minimal Essential Medium (MEM) and Dulbecco’s Modified Eagle Medium/F12 (DMEM/F-12) without phenol red) and supplements (fetal bovine serum (FBS), charcoal-stripped FBS (CD-FBS), L-glutamine and penicillin–streptomycin (P/S)) were purchased from Gibco, Thermo Fisher Scientific, (Waltham/MA, USA). Zearalenone (ZEN), α-zearalenol (α-ZEL), α-zearalanol (α-ZAL), 17-β-estradiol (E2), 4-nitrophenylphosphate, diethanolamine, magnesium chloride and sulforhodamine B (SRB) were obtained from Sigma Aldrich Chemie GmbH (Schnelldorf, Germany), whereas daidzein (DAI), equol (EQ), genistein (GEN) and glycitein (GLY) were purchased from Extrasynthese (Genay Cedex, France). Dimethly sulfoxide (DMSO), NaCl, KCl, Na₂HPO₄, Na₂HPO₄ × 2 H₂O and KH₂PO₄ were purchased from Roth (Karlsruhe, Germany). ZEN-4-sulfate ammonium salt was obtained from Santa Cruz Biotechnology (Dallas/TX, USA) and is the same compound as ZEN-14-sulfate (ZEN-14-S) using the newer International Union of Pure and Applied Chemistry (IUPAC) numbering system (Metzler 2011 (link)). The CellTiter-Blue® Cell Viability Assay Kit was purchased from Promega Corporation (Madison/WI, USA).