To examine the in vivo role of LINC00240, we inoculated subcutaneously a total of 6 × 106 stably LINC00240-OE, LINC00240-KD or control HGC-27 cells into fossa axillaries of five-week-old female nude BALB/c mice (Vital River Laboratory, Beijing, China). Tumor growth was measured every two days as previously described [15 (link), 23 (link)]. To assess functions of LINC00240 during gastric cancer metastases in vivo, we injected a total of 2 × 106 HGC-27 cells with stable LINC00240-KD (shNC, sh240-1, or sh240-2) or LINC00240-OE (NC or LINC00240) into tail vein of 5-week-old female nude BALB/c mice (Vital River Laboratory, Beijing, China) (n = 3 per group). Mouse lungs with metastatic tumors were formalin fixed, paraffin-embedded and stained with hematoxylin and eosin (HE). Immunohistochemistry (IHC) staining was performed in mouse livers with an antibody specific for vimentin as previously reported [24 (link), 25 (link)]. All procedures involving mice were approved by the Animal Care Committee of Shandong Cancer Hospital and Institute. All analyses were performed in a blinded fashion with individuals unaware of xenograft types.
Balb c nude mice
Manufactured by Charles River Laboratories
Sourced in China, Japan, United States, Germany, United Kingdom, France, Italy
BALB/c nude mice are an inbred strain of mice that lack a functional immune system due to a genetic mutation. They are athymic, meaning they lack a thymus gland, which is essential for the development of T cells. This results in a severely compromised adaptive immune response. BALB/c nude mice are commonly used in biomedical research for the study of human diseases, the evaluation of new therapies, and the development of xenograft models.
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Lab products found in correlation
Matrigel, by BD (35 mentions)
Matrigel, by Corning (18 mentions)
Matrigel matrix growth factor reduced, by BD (17 mentions)
Balb c mice, by Charles River Laboratories (15 mentions)
C57bl 6 mice, by Charles River Laboratories (11 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (9 mentions)
Phosphate buffered saline (pbs), by Thermo Fisher Scientific (7 mentions)
Ab15580, by Abcam (7 mentions)
Nod scid mice, by Charles River Laboratories (6 mentions)
Matrigel matrix, by Corning (6 mentions)
C57bl 6j mice, by Charles River Laboratories (6 mentions)
Phosphate buffered saline (pbs), by Solarbio (6 mentions)
Balb c nude mice, by BD (5 mentions)
Isoflurane, by Merck Group (5 mentions)
Sh nc, by Charles River Laboratories (5 mentions)
Sh nc, by GenePharma (5 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (4 mentions)
Matrigel matrix, by BD (4 mentions)
Sh nc, by RiboBio (4 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (3 mentions)
1 574 protocols using balb c nude mice
1
In vivo role of LINC00240 in gastric cancer
2
Investigating Tumor Growth Inhibition by KIF22
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The experiments involving animals were approved by the Institutional Animal Care and Use Committee (IACUC) of the Tianjin Third Central Hospital Affiliated to Nankai University (approval no. SYXK 2019-0318). Nude BALB/c mice were purchased from Beijing Vital River Laboratory Animal Technology Co., Ltd. A total of 16 male Nude BALB/c mice (8 in each group, 8-weeks-old; 18-22 g) were purchased from Beijing Vital River Laboratory Animal Technology Co., Ltd., and fed with food and water ad libitum and at specific pathogen-free conditions (20°C; 60% humidity and alternating 12-h light/dark cycles).
For the tumor growth assay, T24 cells stably transfected with control or KIF22 shRNA lentivirus were subcutaneously injected into the right flanks of female nude mice. Almost 2 weeks later, tumors (150 mm3) were established, and the tumor volume was measured each week and calculated [length x (width)2/2]. The mice were euthanized by an intraperitoneal injection of 120 mg/kg sodium pentobarbital before the tumors were removed at the 49-day time point. The hearts of the mice were then monitored, and death was confirmed by cardiac arrest. Tumor growth curves were plotted according to the tumor volume in the different groups.
For the tumor growth assay, T24 cells stably transfected with control or KIF22 shRNA lentivirus were subcutaneously injected into the right flanks of female nude mice. Almost 2 weeks later, tumors (150 mm3) were established, and the tumor volume was measured each week and calculated [length x (width)2/2]. The mice were euthanized by an intraperitoneal injection of 120 mg/kg sodium pentobarbital before the tumors were removed at the 49-day time point. The hearts of the mice were then monitored, and death was confirmed by cardiac arrest. Tumor growth curves were plotted according to the tumor volume in the different groups.
3
Evaluating CBZ Effects on Bone Microenvironments
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To allow comparison of the effects of CBZ in different bone microenvironments, studies were performed in animal models of different strain, sex and age as follows: 1) 6-week old male BALB/c nude mice, 2) 6-week old female BALB/c nude mice (both Charles River, UK), 3) 8–9-week old female genetically engineered mice expressing GFP-positive cells of the osteoblastic lineage on a BALB/c nude background ((BALB/cAnNCrl.Cg-Tg(Col1a1-GFP)Row Foxn1nu/nu, described in [34] (link), heterozygous, referred to as GFP Ob+ mice) and 4) 17-week old female GFP Ob+ mice (both Leeds Institute for Molecular Medicine, UK).
In murine models, bone remodelling is reduced with increasing age. We therefore established effects of CBZ in young (8–9 week old) mice with high bone turnover in addition to older (17-week old) mice with lower bone turnover.
In murine models, bone remodelling is reduced with increasing age. We therefore established effects of CBZ in young (8–9 week old) mice with high bone turnover in addition to older (17-week old) mice with lower bone turnover.
4
Establishing Tumor-Bearing Nude Mice
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All animal procedures were performed in accordance with the Guidelines for Institutional Animal Care and Use Committee and approved by the Animal Ethics Committee of Shanxi Medical University (No. SYDL2019002). Female BALB/c nude mice (6–8 weeks) with bodyweight of 18–20 g were purchased from Beijing Vital River Laboratory Animal Technology Co. Ltd. BALB/c nude mice were kept under standard housing condition before use. Hep-2 cells (50 μL PBS containing 2.0×106 cells) were subcutaneously implanted on the dorsum region of the right leg to generate tumor-bearing nude mice. When the tumors reached about 100 mm3, these nude mice were conducted for imaging and therapy studies.
5
Murine Tumor Model Establishment
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MC38 cells were purchased from the American Type Culture Collection (ATCC), cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin in 5% CO2 at 37°C. The cells were passaged when they reached 80–90% confluence. Our experiments were performed using C57BL/6 mice (male, 6–8 w) and BALB/c nude mice (male, 6–8 w) weighing 18–22 g. C57BL/6 mice and BALB/c nude mice, purchased from Vital River Laboratory Animal Technology (Beijing, China), were housed in the animal laboratory of First Affiliated Hospital of Shandong First Medical University. All animal experimental procedures were approved by the First Affiliated Hospital of Shandong First Medical University.
6
Nude Mouse Xenograft Model for Colon Cancer
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BALB/c nude mice (aged 4–5 weeks) were obtained from Beijing Vital River Laboratory Animal Technology Co., Ltd. After one week of adaptation, BALB/c nude mice were injected with HT-29 cells to establish a subcutaneous human colon cancer xenograft model. When the tumors grew to approximately 0.5 cm3 in volume, miR-524-5p agomir and the negative control agomir were injected into the tumor every three days. The tumor size was measured every 7 days. Animal studies were performed in compliance with the Guide for the Care and Use of Laboratory Animal Resources (1996), the National Research Council, and approved by the Animal Ethics Committee of the China Medical University (IACUC Issue No. 16071). All procedures were followed under supervision and inspection by the Committee and the Laboratory Animal Department.
7
Sorafenib Efficacy in Huh7 Xenograft Model
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BALB/c nude mice were purchased from Beijing Vital River Laboratory Animal Technology Co., Ltd. Approximately 2 × 106 Huh7 cells transfected with Lv-shNC or Lv-shYB-1 were subcutaneously injected into male BALB/c nude mice aged 4 wk. We measured the tumors every 2 d, and (length × width2)/2 was used to calculate the tumor volume. Next, we randomly divided the mice into designated groups (n = 6) when the average volume of the tumors reached approximately 100 mm3. The nude mice in the Lv-shNC + sorafenib and Lv-shYB-1 + sorafenib groups were treated with sorafenib by gavage at a dosage of 30 mg/kg/d for 14 d. We sacrificed the nude mice when the treatment was completed, and the tissues were fixed with 4% paraformaldehyde or immediately placed at -80 °C. The animal protocol was designed to minimize pain and discomfort to animals. The nude mice were acclimatized to laboratory conditions for 1 wk prior our experiment. Intragastric gavage administration was conducted by using straight gavage needles (22 gauge). All the animals in our study received humane care, and the ethics committee of the Second Hospital of Hebei Medical University approved our study (approval letter No. 2020-AE002).
8
Tumor Xenograft Model for Evaluating Chemotherapeutic Effects
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In this study, 4-6-week-old female BALB/c nude mice were purchased from Vital River Laboratory Animal Technology Co., Ltd (Beijing, China) and divided into 4 groups (n = 6 per group): PBS, DDP, DDP+sh-NC or DDP+sh-circ_0031242. About 2 × 106 sh-NC-infected, sh-circ_0031242-transduced or un-transduced Huh7-R cells were subcutaneously implanted into the left flank of 4-6-week-old female BALB/c nude mice (6 mice per group, Vital River Laboratory, Beijing, China). After one week of implantation, DDP administration (3 mg/kg) was conducted every 4 days by intraperitoneal injection. Tumor growth was periodically assessed by calculating tumor volume as follows: volume = length × width2/2. In the end, tumor tissues were harvested from all sacrificial mice.
9
Tumor growth in BALB/c nude mice
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BALB/c nude mice (male, 5-week-old) were purchased from Beijing Vital River Laboratory Animal Technology Co., Ltd. Then, 100 μl PBS containing 5 × 106 sh-TUG1-1 or sh-NC transfected HemECs cells were subcutaneously inoculated into BALB/c nude mice as previously described [34 (link)] with five mice in each group. Tumor volume was evaluated every week for a total of 5 weeks based on the formula: length × width2/2. On day 35, mice were sacrificed by cervical dislocation, and the tumors were collected and weighted. All animal experiments were approved by the Animal Ethics Committee of Beijing University of Chinese Medicine Shenzhen Hospital (Longgang).
10
Xenograft Tumor Model in Nude Mice
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Nude BALB/c mice (female, 6–8 weeks, 18–22 g, four mice in each group) were purchased from Beijing Vital River Laboratory Animal Technology Co., Ltd. (Beijing, China). PANC-1 PDAC cells (5×106, 100 µL) were injected subcutaneously into the armpit of the nude mice. The size of tumors was measured every 3 days, and the tumor volume was calculated using the formula V = 0.5 × L × W2, where V is the volume, L is the length, and W is the width. After 29 days, the tumors were harvested, weighed, and photographed. All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. Animal research was performed according to guidelines approved by the Tianjin Medical University Cancer Institute and the Hospital Animal Ethics Committee (approval No.: IRM-DWLL-2019113).
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