Measurement principle DCFH-DA (2,7-dichlorofuorescin diacetate) is a fluorescent probe that can pass through freely. After entering the cell, it can be hydrolyzed by intracellular esterase to produce DCFH. Liver tissues were collected and stained using ROS kit and ROS levels in AML12 cells were evaluated through ROS kit following manufacture’s guidance (Elabscience).
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