Ml si1

To determine cell viability, the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was employed. Both ovarian cancer cell lines (2.0 × 10⁴ cells/cm²), treated with 40 μM cisplatin (CDDP; EMD Millipore, Burlington, MA, USA) either alone or in combination with 40 μM ML-SI1 (Sigma Aldrich, St. Louis, MO, USA) for 48 h, were incubated with 2 mg/mL MTT solution at 37 °C for 3 h in the dark. Subsequently, dimethyl sulfoxide (DMSO) was added to dissolve the formazan crystals produced by viable cells. The absorbance was measured at 540 nm by a microplate reader (SpectraMax^® ABS, Molecular Devices, San Jose, CA, USA).

To assess cell viability, the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was utilized. Pancreatic cancer cells were treated with ML-SI1 (Sigma Aldrich, St. Louis, MO, USA) for 48 h and then exposed to 2 mg/mL MTT solution at 37 °C for 3 h in the dark. Upon completion of the incubation period, dimethyl sulfoxide (DMSO) was added to dissolve the formazan crystals generated by viable cells. The absorbance was subsequently measured at 540 nm using a microplate reader (SpectraMax^® ABS, Molecular Devices, San Jose, CA, USA).