Saslab pro

Manufactured by Avisoft

Sourced in Germany

SASLab Pro is a comprehensive software package designed for advanced sound analysis. It offers a range of tools for recording, editing, and analyzing acoustic signals. The software supports a variety of file formats and provides advanced signal processing capabilities, including spectral analysis, time-frequency analysis, and signal segmentation.

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Lab products found in correlation

Cm16 cmpa, by Avisoft (12 mentions) Recorder software, by Avisoft (12 mentions) Ultrasoundgate 416h, by Avisoft (8 mentions) Ultrasoundgate condenser microphone capsule cm16, by Avisoft (8 mentions) Ultrasoundgate 116h, by Avisoft (6 mentions) Ultrasoundgate condenser microphone cm 16, by Avisoft (5 mentions) Ultrasoundgate416, by Avisoft (4 mentions) Ultrasoundgate 116hb, by Avisoft (3 mentions) Ultrasoundgate cm16, by Avisoft (3 mentions) Ausg 116h, by Avisoft (3 mentions) Ethovision xt, by Noldus (2 mentions) Ultrasoundgate 116, by Avisoft (2 mentions) Ultrasoundgate cm16 cmpa, by Avisoft (2 mentions) Ultrasoundgate, by Avisoft (2 mentions) Sound attenuating chamber, by Med Associates (2 mentions) Ultrasoundgate 116hme microphone, by Avisoft (2 mentions) Recorder usgh, by Avisoft (2 mentions) Ecm ms957, by Sony (1 mentions) Ultrasonic dynamic speaker vifa, by Avisoft (1 mentions) Recorder usgh software, by Avisoft (1 mentions)

137 protocols using saslab pro

Quantification of Ultrasonic Vocalizations in Male Mice

Cited 3 times

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Each male from one of the four treatment groups was tested in its home cage in the presence of a sexually receptive female as previously described.^{3 (link),13 (link),17 (link),19 (link),20 (link)} The 4-min recording of vocalizations started after the introduction of the female, using an UltraSoundGate microphone (Avisoft Bioacoustics), which was connected to a computer equipped with the Avisoft-SASLab Pro (Avisoft Bioacoustics) recording software. Avisoft-SASLab Pro (Avisoft Bioacoustics) was used to analyze USVs and generate spectrograms for each detected call. Emitted syllables were grouped into simple, complex, and frequency-jump categories based on their shape and frequency.^{3 (link),17 (link)} The total number and duration of USVs and the number and duration of each syllable were quantified.

Ducroq S., Duplus E., Penalva-Mousset L., Trivelloni F., L’honoré A., Chabat-Courrède C., Nemazanyy I., Grange-Messent V., Petropoulos I, & Mhaouty-Kodja S. (2023). Behavior, Neural Structure, and Metabolism in Adult Male Mice Exposed to Environmentally Relevant Doses of Di(2-ethylhexyl) Phthalate Alone or in a Phthalate Mixture. Environmental Health Perspectives, 131(7), 077008.

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Ultrasonic Vocalizations in Rodent Courtship Behavior

Cited 2 times

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Each male was tested in its home cage in the presence of a female from one of the four treatment groups as previously described (Capela et al. 2018 (link), 2019 (link); Dombret et al. 2017 (link)). After the introduction of a female, vocalizations were recorded for 4 min with an UltraSoundGate microphone (Avisoft Bioacoustics), which was connected to an ultrasound recording interface plugged into a computer equipped with the Avisoft-SASLab Pro (version 5.2.09; Avisoft Bioacoustics) recording software. Vocalizations were analyzed using Avisoft-SASLab Pro (Avisoft Bioacoustics). Spectrograms were generated for each detected call (frequency resolution: FFT-length: 512; frame size: 100%; overlap: 50%). The parameters used for the automatic quantification of the vocalizations were: cutoff frequency of

30 kHz

, element separation based on an automatic single threshold with a hold time of

15 ms

. Syllables were identified and grouped into three main categories (simple, complex, frequency-jump). The total number and duration of USVs were analyzed, as well as the number and duration of each syllable.

Adam N., Brusamonti L, & Mhaouty-Kodja S. (2021). Exposure of Adult Female Mice to Low Doses of di(2-ethylhexyl) Phthalate Alone or in an Environmental Phthalate Mixture: Evaluation of Reproductive Behavior and Underlying Neural Mechanisms. Environmental Health Perspectives, 129(1), 017008.

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Ultrasonic Vocalization Analysis Protocol

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All ultrasonic vocalizations collected were analyzed using Avisoft SASLab Pro software (Avisoft SASLabPro, RRID:SCR_014438). The parameters consisted of: a fast Fourier transformation (FFT) length of 1024, time window overlap of 75%, a 100% hamming window, a time resolution of 1 ms, and a sampling frequency of 250 kHz, per established protocol (Scattoni et al., 2008 ). Files were cleaned by a trained experimenter that was blinded to the treatment condition and identity of the animal. Each file was manually assessed, and each vocalization was identified using the Avisoft software. Any extraneous noise was manually removed from the spectrogram.

Binder M., Nolan S.O, & Lugo J.N. (2020). A comparison of the Avisoft (v.5.2) and MATLAB Mouse Song Analyzer (v.1.3) vocalization analysis systems in C57BL/6, Fmr1-FVB.129, NS-Pten-FVB, and 129 mice. Journal of neuroscience methods, 346, 108913.

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Acoustic Analysis of Horse Whinnies

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Based on a previous definition [23 ], and on the acoustic variation of the whinnies that we recorded, we defined a whinny as a call with three or more rises and falls in the frequency domain (i.e., modulations) that can range between ca. 300 to 5,300 Hz, with a duration of ca. 0.4 to 3.5 s (Fig 2). In total, we analyzed the acoustic variation of 566 whinnies produced by 35 individuals (S1 Table), and extracted 42 acoustic parameters which were selected based on previous studies and acoustic qualities considered helpful to describe whinny variation [24 –26 ] (S2 Table). To analyze acoustic parameters, we used Avisoft version 5.2.05 (Avisoft SASLab Pro) with a 16 kHz sampling frequency, a fast Fourier transform of 256 samples, and a Hamming window with an overlap of 96.9%. In addition, we measured the separation time between the starts of whinnies produced by focal animals, and the start of the whinnies produced by other animals within a ±10 s range (Fig 2B).

Ordóñez-Gómez J.D., Santillan-Doherty A.M, & Hammerschmidt K. (2019). Acoustic variation of spider monkey (Ateles geoffroyi) contact calls is related to caller isolation and affects listeners’ responses. PLoS ONE, 14(4), e0213914.

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Acoustic Monitoring of Bat Biodiversity

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Acoustic recordings were conducted along the edges of each plot, using a combination of point-stop and transect monitoring in 2009 and 2010. Point-stops were located at each corner of the plot. Transects were walked slowly and in a direct line between the point stops. Survey time at point-stops and during transect walks were 3 min each. This resulted in a total survey time of 24 min along a 200 m transect per plot. Recordings were made using a Pettersson D 1000× ultrasound detector (Pettersson Electronic AG, Uppsala, Sweden). Echolocation calls were identified to species level or to Sonotype using the software Avisoft SAS Lab Pro, Version 5.0.24 and onward (Raimund Specht, Avisoft Bioacoustics, Berlin Germany). Records were available for 148 plots in total, including 134 in 2009 and 130 in 2010. Open datasets from Biodiversity Exploratories: refs. ^{118 ,119}.

Neyret M., Le Provost G., Boesing A.L., Schneider F.D., Baulechner D., Bergmann J., de Vries F.T., Fiore-Donno A.M., Geisen S., Goldmann K., Merges A., Saifutdinov R.A., Simons N.K., Tobias J.A., Zaitsev A.S., Gossner M.M., Jung K., Kandeler E., Krauss J., Penone C., Schloter M., Schulz S., Staab M., Wolters V., Apostolakis A., Birkhofer K., Boch S., Boeddinghaus R.S., Bolliger R., Bonkowski M., Buscot F., Dumack K., Fischer M., Gan H.Y., Heinze J., Hölzel N., John K., Klaus V.H., Kleinebecker T., Marhan S., Müller J., Renner S.C., Rillig M.C., Schenk N.V., Schöning I., Schrumpf M., Seibold S., Socher S.A., Solly E.F., Teuscher M., van Kleunen M., Wubet T, & Manning P. (2024). A slow-fast trait continuum at the whole community level in relation to land-use intensification. Nature Communications, 15, 1251.

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Ultrasonic Vocalizations in Rats

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Ultrasonic vocalizations (USVs) were recorded by use of a microphone (Type 4158 N, Aco, Tokyo, Japan) designed for measurements of sound pressure levels of 20–100,000 Hz sounds. The microphone was placed at a distance of approximately 20 cm from a rat and was connected to a sensor amplifier (SR-2200, Ono Sokki, Kanagawa, Japan). Acoustic data were recorded at a sampling rate of 200 kHz and with 16 bits by an Avisoft RECORDER (Version 4.2, Avisoft Bioacoustics, Berlin, Germany) and analyzed by a software program of Avisoft SASLab Pro (Version 5.2, Avisoft Bioacoustics). Spectrograms were generated with a fast Fourier transform length of 256 points and an overlap of 75%.

Okabe S., Takayanagi Y., Yoshida M, & Onaka T. (2020). Gentle stroking stimuli induce affiliative responsiveness to humans in male rats. Scientific Reports, 10, 9135.

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Acoustic Analysis of Animal Calls

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All calls from the 72 selected 10-s sections (36 per recording context) that had no background noise were analyzed in Avisoft-SAS Lab Pro (Version 5.2.05; Avisoft Bioacoustics, Glienicke, Germany) according to the methods described in Leliveld et al. (2017 (link)). A short description of the settings used and parameters measured is provided in the Supplementary Material. In total, 222 calls in the IC sections and 68 calls in the RC sections could be analyzed. Twenty-one calls consisted of two distinctly different acoustic structures (mainly grunt-squeals), which were analyzed separately.

Leliveld L.M., Düpjan S., Tuchscherer A, & Puppe B. (2020). Hemispheric Specialization for Processing the Communicative and Emotional Content of Vocal Communication in a Social Mammal, the Domestic Pig. Frontiers in Behavioral Neuroscience, 14, 596758.

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Acoustic Analysis of Ultrasonic Vocalizations

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Microphones were placed 30 cm from the apparatus floor with one microphone placed in the bark chamber and one microphone placed in the ambient noise chamber. Microphone channels were calibrated to equal gain (-60 dB noise floor) and WAV files were produced using RECORDER software (Avisoft Bioacoustics, Berlin, Germany). Recordings were made using a 250 kHz sampling rate with 16-bit resolution and spectrograms were produced with a 512 fast Fourier transform made using Avisoft SASLab Pro (Avisoft bioacoustics). SVs are low-bandwidth calls with low modulation, a peak frequency of 20 kHz and a duration of 100 to 500 ms for each individual syllable. Sweep calls are relatively short upward or downward frequency-modulated calls that can be either simple, lacking inflection, or complex, including inflections [25 (link)]. Both the total number of USV calls produced and the proportion of each USV individual call type produced relative to all call type production were analyzed within this dataset.

Rieger N.S., Monari P.K., Hartfield K., Schefelker J, & Marler C.A. (2021). Pair-bonding leads to convergence in approach behavior to conspecific vocalizations in California mice (Peromyscus californicus). PLoS ONE, 16(8), e0255295.

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Avian Song Playback Protocol

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The stimuli were created using Avisoft-SAS Lab Pro software version 5.0 Germany (Avisoft Bioacoustics, Berlin, Germany). From the recordings made at dawn during the egg-laying period in 2011, we selected a simple two-notes song type from each subject male. Based on the quality of the recording (signal-to-noise ratio), a strophe of approximately 3 s per song type was selected. The selected strophes were trimmed, filtered at 1500 Hz, and their amplitude was normalized at 75% of a volt. A silent gap of 3 s was inserted after each strophe, and both strophe and gap were copied and appended several times in order to create a five-minute lopping song.

Rivera-Gutierrez H.F., Martens T., Pinxten R, & Eens M. (2017). Learning speed is affected by personality and reproductive investment in a songbird. PLoS ONE, 12(10), e0185410.

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Pup Isolation and Ultrasonic Vocalizations

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Pups (P2–P14) were isolated from their mother and littermates for 10 min. For testing, pups were gently placed into a glass isolation container containing clean bedding material and surrounded by a sound attenuating box (18 × 18 × 18 cm) made of 4-mm-thick Styrofoam. An ultrasound Gate system monitored ultrasonic vocalization (USV) emission. Acoustic data were recorded with a sampling rate of 250,000 Hz in 16-bit format by a recorder (version 2.97; Avisoft Bioacoustics). For acoustical analysis, recordings were transferred to Avisoft SASLab Pro (version 4.50; Avisoft Bioacoustics)

Pandian S., Zhao J.P., Murata Y., Bustos F.J., Tunca C., Almeida R.D, & Constantine-Paton M. (2020). Myosin Va Brain-Specific Mutation Alters Mouse Behavior and Disrupts Hippocampal Synapses. eNeuro, 7(6), ENEURO.0284-20.2020.

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