Sc 9970

Manufactured by Santa Cruz Biotechnology

Sc-9970 is a laboratory instrument manufactured by Santa Cruz Biotechnology. It is designed for performing specific analytical procedures in a research setting. The core function of Sc-9970 is to facilitate precise measurements and data collection required for various scientific experiments and analyses.

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Lab products found in correlation

Bs 0646r, by Bioss Antibodies (1 mentions) Bs 0778r, by Bioss Antibodies (1 mentions) Sc 377246, by Santa Cruz Biotechnology (1 mentions) Ab81032, by Abcam (1 mentions) Sc 6597, by Santa Cruz Biotechnology (1 mentions)

2 protocols using sc 9970

Immunophenotyping of Renal Cells and Tissues

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Cells were fixed in 4% paraformaldehyde for 30 min followed by washing for three times using PBS. Cells were treated with 0.1% Triton X-100 for 15 min and blocked using 5% BSA (Hyclone, SH30574.03) for 1 h. For renal tissue, specimen was fixed in 4% paraformaldehyde for 24 h or more. Through the production of paraffin sections and antigen retrieval, tissue sections were blocked using 5% BSA for 1 h. Then, cells and sections were incubated using primary against CD29 (sc-9970, 1:200, Santa Cruz), CD34 (bs-0646R, 1:500, bioss), CD44 (bs-4916R, 1:400, bioss), CD45 (bs-0522R, 1:300, bioss), CD90 (bs-0778R, 1:500, bioss), nephrin (sc-377246, 1:100, Santa Cruz), LC3 (12135-1-AP, 1:200, Proteintech) at 4 °C overnight. After incubation with secondary antibodies at 37 °C for 1 h, samples or cells were stained with DAPI for 5 min and laser confocal microscopy was adopted for observation and photo taking.

Jin J., Shi Y., Gong J., Zhao L., Li Y., He Q, & Huang H. (2019). Exosome secreted from adipose-derived stem cells attenuates diabetic nephropathy by promoting autophagy flux and inhibiting apoptosis in podocyte. Stem Cell Research & Therapy, 10, 95.

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Integrin Modulation of MSC Secretion

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MSC were serum-starved (0.5% FBS) overnight and pretreated for 30 min with antibodies (10 μg/mL) specific to integrin α₆ (sc-6597, Santa Cruz Biotechnology), integrin β₁ (sc-9970, Santa Cruz Biotechnology), integrin α₂β₁ (sc-59955, Santa Cruz) or a non-specific isotype control antibody (ab81032, Abcam). Cells were then rinsed with PBS, trypsinized and suspended in 2% alginate gels at 5×10⁶ cells/mL.
Hydrogel disks were formed with an 8 mm biopsy punch and placed in 24 well plates containing growth media (α-MEM containing 10% FBS and 1% P/S). Media were collected on day 7 after refreshing the media 24 hours prior to collection. Cell content within the gel was measured by quantifying DNA. VEGF concentration within conditioned media was quantified by ELISA.

Jose S., Hughbanks M.L., Binder B.Y., Ingavle G.C, & Leach J.K. (2014). Enhanced trophic factor secretion by mesenchymal stem/stromal cells with Glycine-Histidine-Lysine (GHK)-modified alginate hydrogels. Acta biomaterialia, 10(5), 1955-1964.

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