Anti trpm7

Total RNA isolation, cDNA synthesis and PCR amplification for the following genes, immunoblotting and quantification of immuno-detectable bands were performed as previously described ^{20 (link)}. Primary antibodies used include: anti-pSTAT3/tSTAT3, anti-pJAK2(Tyr1007/1008)/tJAK2, anti-pAKT/tAKT (Cell Signaling Technology, Danvers, MA), anti-TRPM7 (Abcam, catalog number: ab85016, Cambridge, MA), anti-CD133, anti-ALDH1, anti-Hey2, ati-Survivin and anti-β-actin (Sigma-Aldrich, St. Louis, MO). PCR primers used for TRPM7 (5′-3′) were: GCCACCAAGGAGGTACACAT and CTTCCAGGGCCGTGTAGATA, ALDH1 (5′-3′) were: CCCTTTGGTGGATTCAAGAT and TTGAAGAGCTTCTCTCCACTCTT, CD133 (5′-3′) were, CAGAAGGCATATGAATCCAAAA and ATAAACAGCAGCCCCAGGAC, Notch1 (5′-3′) were, CAC TGT GGGCGGGTCC and GTTGTATTGGTTCGGCACCAT, Survivin (5′-3′) were, GCCCAGTGTTTCTTCTGCTT and CCTCCCAAAGTGCTGGTATT, Hey2 (5′-3′) were, AAAAAGCTGAAATATTGCAAATGA and GTACCGCGCAACTTCTGTT, Jagged1(5′-3′) were, GACTCATCAGCCGTGTCTCA and TGGGGAACACTCACACTCAA, WNT1(5′-3′) were GCGCTTCCTCATGAACCTT and GTGCATGAGCCGACATC, Notch2(5′-3′) were, AATCCCTGACTCCAGAACG and TGGTAGACCAAGTCTGTGATG AT, Sox11(5′-3′) were, GACCCAGACTGGTGCAAGAC and GCCCAGCCTCTTGGAGAT, GAPDH (5′-3′) were, GAAGGTGAAGGTCGGAGTC and GAAGATGGTGATGGGATTTC.