Raw single-walled carbon nanotubes produced by the HiPco process (Nanointegris) were used throughout this study. For each dispersion, 1 mg of raw nanotubes was added to 2 mg of (GT)n (where n = 6, 9, 12, 15, or 30) oligonucleotide (Integrated DNA Technologies), suspended in 1 mL of 0.1 M NaCl (Sigma-Aldrich), and ultrasonicated using a 1/8 in. tapered microtip for 30 min at 40% amplitude (Sonics Vibracell VCX-130; Sonics and Materials). The resultant suspensions were ultracentrifuged (Sorvall Discovery M120 SE) for 30 min at 250 000×g and the supernatant was collected. Concentrations were determined using a UV/vis/NIR spectrophotometer (Jasco, Tokyo, Japan) and the extinction coefficient of A910 = 0.02554 L mg−1 cm−1.15 (link)
Uv vis nir spectrophotometer
Manufactured by Jasco
Sourced in Japan
The UV/vis/NIR spectrophotometer is a laboratory instrument used to measure the absorption or transmission of light by a sample across the ultraviolet, visible, and near-infrared regions of the electromagnetic spectrum. It provides quantitative analysis of the sample's composition or concentration.
Automatically generated - may contain errors
Lab products found in correlation
Vibra cell, by Sonics (4 mentions)
Amicon centrifuge filter, by Merck Group (4 mentions)
Oligonucleotide, by Integrated DNA Technologies (3 mentions)
V 570, by Jasco (3 mentions)
Sodium chloride (nacl), by Merck Group (3 mentions)
Fp 6200 spectrophotometer, by Jasco (2 mentions)
J 820 spectropolarimeter, by Jasco (2 mentions)
Ft ir 4200 plus spectrophotometer, by Jasco (2 mentions)
Vibra cell vcx130, by Sonics (1 mentions)
500 mhz instrument, by Bruker (1 mentions)
Ltq orbitrap xl, by Thermo Fisher Scientific (1 mentions)
X pert pro powder x ray diffractometer, by Malvern Panalytical (1 mentions)
3flex analyzer, by Micromeritics (1 mentions)
100 mm nacl, by Merck Group (1 mentions)
12 protocols using uv vis nir spectrophotometer
1
Dispersing Carbon Nanotubes with Oligonucleotides
2
Characterization of Organic Compounds
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1H and 13C NMR spectra were recorded on a Bruker 500 MHz instrument (Billerica, MA, USA) with tetramethylsilane (TMS) as an internal standard. MS data were obtained using either a JEOL-700 MStation (EI, Akishima, Tokyo, Japan) or a Thermo Scientific LTQ Orbitrap XL (Waltham, MA, USA) with negative ion mode nanoelectrospray ionization (nESI−). Infrared (IR) spectra were recorded on a Perkin-Elmer FTIR (Waltham, MA, USA). UV-visible spectra were obtained using a JASCO UV/Vis/NIR spectrophotometer (Tokyo, Japan). Optical bandgaps (Eg) were determined using the edge of the longest wavelength absorption (λ) using Eg = 1240/λ. A three-electrode system consisting of a Pt working electrode, a Pt wire counter electrode, and an Ag wire pseudoreference electrode was used for cyclic voltammetry measurements using a CHI 440A electrochemical analyzer (Austin, TX, USA). The redox potential of the dyes was measured in DMF containing 0.1 M tetrabutylammonium hexafluorophosphate, and the potential was calibrated against the ferrocene/ferrocenium (Fc/Fc+). Before recording the electrochemical data, the solutions were carefully purged with nitrogen gas.
3
UV-Vis Diffuse Reflectance Spectroscopy
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UV–visible diffuse reflectance spectroscopy (DRS) assessments were conducted using a Jasco V570 UV–vis NIR spectrophotometer equipped with an integration sphere diffuse reflectance accessory, originating from the USA.
4
DNA-Stabilized Carbon Nanotube Dispersion
5
Characterization of ZnL Interaction with HSA
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Infrared (IR) spectra were recorded by transmission mode, using KBr pellets for ZnL only and by reflection mode as cast films for HSA and HSA+ZnL using a stainless plate on a JASCO (Tokyo, Japan) FT-IR 4200 plus spectrophotometer in the range 4000–400 cm−1 at 298 K. Electronic (UV-Vis) spectra were obtained on a JASCO (Tokyo, Japan) V-570 UV-vis-NIR spectrophotometer in the range 1500–200 nm at 298 K. Fluorescence spectra were measured on a JASCO (Tokyo, Japan) FP-6200 spectrophotometer in the range of 720–220 nm. Circular dichroism (CD) spectra were obtained on a JASCO (Tokyo, Japan) J-820 spectropolarimeter in the range 900–250 nm at 298 K.
6
DNA-Wrapped Single-Walled Carbon Nanotube Preparation
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SWCNTs produced by the HiPco process (Nanointegris) were used throughout the study. SWCNTs were dispersed with single-stranded DNA oligonucleotides in 1 mL of 100 mM NaCl (Sigma-Aldrich), by adding 1 mg of raw nanotubes to 2 mg of desalted (GT)n (n = 3, 6, 9, 12, 15, or 30), (CT)15, or C30 oligonucleotide (Integrated DNA Technologies) in a microcentrifuge tube. The mixtures were ultrasonicated using a 1/8” tapered microtip (Sonics Vibracell; Sonics & Materials) for 30 minutes at 40% amplitude, with an average power output of 8 W, in a 0 °C temperature-controlled microcentrifuge tube holder. After sonication, the dispersion was ultracentrifuged (Sorvall Discovery M120 SE) for 30 minutes at 250,000 g, and the top 80% of the supernatant was extracted. The concentration was determined with a UV/vis/NIR spectrophotometer (Jasco, Tokyo, Japan), using the extinction coefficient of A910 = 0.0255455 (link) L.mg−1.cm−1. The free DNA was removed using 100 kDa Amicon centrifuge filters (Millipore). For each sample, filtration was repeated 4 times, and the DNA-SWCNTs pellet was resuspended in DI water in each step including the final step.
7
Dispersing SWCNTs with DNA Oligonucleotides
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The chemical reagents were purchased from Sigma-Aldrich
(St. Louis,
MO, USA) and Fisher Scientific (Pittsburgh, PA, USA). Single-walled
carbon nanotubes produced by the HiPco process were used throughout
the study (Unidym, Sunnyvale, CA, USA). The carbon nanotubes were
dispersed with DNA oligonucleotides via probe-tip
ultrasonication (Sonics & Materials, Inc.) of 2 mg of the specified
oligonucleotide (IDT DNA, Coralville, IA, USA) with 1 mg of raw SWCNT
in 1 mL of 0.1 M NaCl for 30 min at 40% of the maximum amplitude of
the ultrasonicator (SONICS Vibra Cell). Following ultrasonication,
the dispersions were ultracentrifuged (Sorvall Discovery 90SE) for
30 min at 280 000g. The top three-fourths
of the resultant supernatant was collected, and its concentration
was determined with a UV/vis/NIR spectrophotometer (Jasco, Tokyo,
Japan) using the extinction coefficient Abs910 = 0.02554
L mg–1cm–1.19 (link) To remove free DNA, 100 kDa Amicon centrifuge filters (Millipore)
were used to concentrate and resuspend the DNA–nanotube complexes.
(St. Louis,
MO, USA) and Fisher Scientific (Pittsburgh, PA, USA). Single-walled
carbon nanotubes produced by the HiPco process were used throughout
the study (Unidym, Sunnyvale, CA, USA). The carbon nanotubes were
dispersed with DNA oligonucleotides via probe-tip
ultrasonication (Sonics & Materials, Inc.) of 2 mg of the specified
oligonucleotide (IDT DNA, Coralville, IA, USA) with 1 mg of raw SWCNT
in 1 mL of 0.1 M NaCl for 30 min at 40% of the maximum amplitude of
the ultrasonicator (SONICS Vibra Cell). Following ultrasonication,
the dispersions were ultracentrifuged (Sorvall Discovery 90SE) for
30 min at 280 000g. The top three-fourths
of the resultant supernatant was collected, and its concentration
was determined with a UV/vis/NIR spectrophotometer (Jasco, Tokyo,
Japan) using the extinction coefficient Abs910 = 0.02554
L mg–1cm–1.19 (link) To remove free DNA, 100 kDa Amicon centrifuge filters (Millipore)
were used to concentrate and resuspend the DNA–nanotube complexes.
8
Dispersing HiPco SWCNTs with DNA
9
Spectroscopic Characterization of Zn-HSA Complexes
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Infrared (IR) spectra were recorded by transmission mode using KBr pellets for Zn complex only and by reflection mode as cast films for HSA and HSA+Zn complexes using a stainless plate on a JASCO (Tokyo, Japan) FT-IR 4200 plus spectrophotometer in the range 4000–400 cm−1 at 298 K. Electronic (UV-vis) spectra were obtained on a JASCO (Tokyo, Japan) V-570 UV-vis-NIR spectrophotometer in the range 1500–200 nm at 298 K. Fluorescence spectra were measured on a JASCO (Tokyo, Japan) FP-6200 spectrophotometer in the range of 720–220 nm. Circular dichroism (CD) spectra were obtained on a JASCO (Tokyo, Japan) J-820 spectropolarimeter in the range 900–250 nm at 298 K.
10
Analytical Characterization of DPC-Cx Materials
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Scanning transmission electron microscopy (STEM) analysis was carried out on an FEI-TITAN operated at an accelerating voltage of 300 kV. For sample preparation, powders of the DPC-Cx were dispersed in ethanol with the assistance of sonication for 10 s, and a drop of solution was dropped onto a holey carbon-coated 200 mesh TEM grid. Excess liquid was immediately wicked away with blotting paper and then air dried before exposing the sample to an electron beam and plasma cleaning for 5 s. X-ray diffraction patterns were recorded using a Panalytical X'Pert Pro powder X-ray diffractometer using Cu-Kα radiation. UV-DRS measurements were carried out using a JASCO UV/vis/NIR spectrophotometer. The surface area was obtained using the Brunauer–Emmett–Teller (BET) theory from N2 physisorption data recorded using a Micromeritics 3Flex analyzer. Approximately 100 mg of each sample was degassed at 120 °C for 12 h prior to N2 sorption analysis.
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