The samples were prepared according to the modified method of Kim et al. (2016) [15 (link)]. The samples were weighed (5 g each) into a 50 mL conical tubes, rinsed with 15 mL of n-hexane and then transferred to a separatory funnel containing 5 mL of n-hexane, followed by extraction with 150 mL portions of n-hexane-saturated acetonitrile. For preparing the processed meat products and the dressing samples, the organic solvent layer was transferred to another separatory funnel to remove the residues which interfered with the separations. The acetonitrile phase was collected in a concentrate flask, and then evaporated to a volume of 3 to 4 mL using a water bath (≤40 °C, EYELA, SB-1200, Tokyo, Japan) with a vacuum rotary evaporator (EYELA, N-1200A, Tokyo, Japan). The flask was rinsed with small portions of solvent (acetonitrile:iso-propanol, 1:1, v/v) which were then transferred to a 10 mL volumetric flask. The rinsing step was repeated until exactly 10 mL was collected in volumetric flask. The samples were filtered through a 0.45 μm syringe filter (Millex-HV, Millipore, Bedford, MA, USA).
Sb 1200
Manufactured by Eyela
Sourced in Japan, China
The SB-1200 is a laboratory shaker designed for gentle mixing and agitation of samples. It features a compact and robust construction, with a maximum shaking speed of 200 RPM and a maximum load capacity of 12 kg. The device is suitable for a variety of applications, including cell culture, microbiological studies, and biochemical assays.
Automatically generated - may contain errors
Lab products found in correlation
Whatman, by Cytiva (2 mentions)
N 1110v w, by Eyela (1 mentions)
Xl 80 ultracentrifuge, by Beckman Coulter (1 mentions)
Multiskan go, by Thermo Fisher Scientific (1 mentions)
Freexeone 4, by Labconco (1 mentions)
Scanvac, by Labogene (1 mentions)
Kinetic chromogenic limulus amebocyte lysate assay, by Lonza (1 mentions)
Econo column, by Bio-Rad (1 mentions)
Fraction collector, by Gilson (1 mentions)
15 protocols using sb 1200
1
Extraction and Purification of Analytes
2
Niosomal Formulations for MEO Delivery
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The formulations were prepared using film hydration method as previously described (20 ). A precise amount of 1200 μmol of the nonionic surfactants (Span and Tween) and Chol in different molar ratios, 50:50, 60:40, and 70:30 were dissolved in chloroform in a round-bottom flask. Afterwards, 2.5 mL of MEO solution 2% in chloroform was added to the lipid phase. After removing the organic solvents at 50 °C under vacuum in a rotary evaporator (EYELA SB-1200, Japan), the film was hydrated with 10 mL deionized water with a gentle rotation at 50 °C for 60 min to produce an aqueous niosomal suspension containing 0.5% MEO.
3
Thin Film Hydration Method for Proliposome Preparation
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The thin film hydration method19 (link),20 (link) was adopted as the conventional method used to prepare proliposomes for comparison with proliposomes containing CsA that were prepared by a novel SAS process. Phospholipids, cholesterol, and CsA were dissolved in organic solvents, followed by sonication (Ultrasonic Cleaner UC-20; Jeio Tech Co., Ltd., Seoul, Republic of Korea) until a clear and homogeneous solution was obtained. Anhydrous lactose was then transferred to a round-bottomed flask into which the drug–lipid solution was slowly poured. The flask was then connected to a rotary evaporator (N-1110V-W; EYELA, Shanghai, People’s Republic of China) and water bath (SB-1200; EYELA), with the temperature maintained at 45°C for Film-EPCS and 60°C for Film-DSPG proliposomes with mixing. The organic solvent was then removed by reduced pressure and temperature to obtain a film on the wall of the vessel. Proliposomes were then collected and stored at 4°C.
4
Methanol Extraction of Plant Material
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The same plant material as PLs (50 g) was extracted with MeOH (500 mL × 2) under reflux for 2 h. The solvent was removed using a rotary vacuum evaporator (EYELA SB-1200) at 40 °C to yield a MeOH extract (PLr, 7.9 g).
5
Soft Cheese Production with Lactic Acid Bacteria
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Fresh soft cheese samples were prepared with or without LAB according to a modified general cheese manufacturing method (Chandan et al., 2015) . Pasteurized whole milk was purchased from a local market and heated to 37°C in a water bath (SB-1200, Eyela, Tokyo, Japan). We added LcM or LbC to milk as a starter culture at 10 5 to 10 6 cfu/mL, mixed for 1 min, and let ferment at 40°C for 1 h. We added 0.02% of rennet (Maysa, Istanbul, Turkey) and 0.2% of citric acid (Weifang Ensign Industry Co. Ltd., Weifang, China) to coagulate the milk. After coagulation, samples were cut to separate the curd and whey. Cooled fresh soft cheese was then pressurized. Figure 1 shows the flowchart of fresh soft cheese manufacturing with processing treatments.
6
Liposomal Amphotericin B Preparation
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The liposomal AmB was also prepared by the conventional thin film hydration method for comparison with the liposomes that were prepared by the SCF-CO2 method. AmB was dissolved in an organic solvent and mixed with a solution of phospholipid dissolved in organic solvents in a manner that was similar to that in the SCF-CO2 method. The mixture was then transferred to a round-bottom flask and connected to an EYELA rotary evaporator (N-1110V-W; EYELA, Shanghai, China) and water bath (SB-1200; EYELA), with the temperature being maintained at 45 °C with proper mixing. The organic solvents were then removed under reduced pressure to obtain a thin film on the wall of the vessel; the resulting film was hydrated with 9% lactose aqueous solution at a specific temperature of 65 °C. After hydration, multilamellar liposomal AmB was obtained, and the resulting liposomes were sonicated (Beckman XL-80 ultracentrifuge, Branson, MO, USA) for 30 min. for vesicle size reduction.
7
Extraction and Characterization of Yacon Leaves
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The Yacon (Smallanthus sonchifolius ((Poepp. & Endl.) H. Rob.) leaves were collected from Pindaya Township, Shan State, in the eastern part of Myanmar. The sample was identified at the Department of Botany, University of Yangon. The leaves were cleaned, carefully dried in shadow, and powdered. About 2 g of ground powder was soaked in 20 mL methanol and shaken for 8 h at 180 rpm and 37 °C. The soaked substance was filtered throughout 110 mm filter paper (Hyundai, Seoul, South Korea). The solvent was eliminated with a rotary evaporator (SB-1200, EYELA, Shanghai, China) at 60 °C. The residue was placed in a freeze drier (Operon, Korea) to dry. The crude extract was kept in a refrigerator at 4 °C and protected from light. The experiment was performed in triplicate. Four-hundred mg of the sample extract was dissolved in 1 mL of dimethyl sulfoxide (DMSO) and then serially diluted to 100, 60, 40, and 20 μg/mL for further biological experiments. All other chemical reagents were from Sigma-Aldrich Chemical Company (St. Louis, MO, USA) unless otherwise noted.
8
Antioxidant Activity of Albumen Extract
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After blending the albumen and 95% ethanol at a ratio of 1:10 (w/v), the
mixture was extracted at 60°C in a water bath (SB-1200, EYELA, Shanghai,
China) with continuous shaking at a speed of 170 r/min for 2 h. After
extraction, the mixture was centrifuged at 2,090×g for 10 min, and the
supernatant was used for DPPH radical scavenging activity analysis (Harlina et al., 2019 (link)). The DPPH radical
scavenging activity was analyzed by slight modification of the method reported
by Blois (1958) (link). One hundred microliters
of the sample was combined with 100 μL of 0.2 mM DPPH reagent and kept in
the dark for 30 min. The absorbance of the reactant was then measured at 517 nm
with a spectrophotometer (Multiskan GO, Thermo Fisher Scientific, MA, USA).
Radical scavenging activity was expressed as percentage according to the
following equation:
Where, A1 is the absorbance of samples, and
A0 is the absorbance of control (distilled
water).
mixture was extracted at 60°C in a water bath (SB-1200, EYELA, Shanghai,
China) with continuous shaking at a speed of 170 r/min for 2 h. After
extraction, the mixture was centrifuged at 2,090×g for 10 min, and the
supernatant was used for DPPH radical scavenging activity analysis (Harlina et al., 2019 (link)). The DPPH radical
scavenging activity was analyzed by slight modification of the method reported
by Blois (1958) (link). One hundred microliters
of the sample was combined with 100 μL of 0.2 mM DPPH reagent and kept in
the dark for 30 min. The absorbance of the reactant was then measured at 517 nm
with a spectrophotometer (Multiskan GO, Thermo Fisher Scientific, MA, USA).
Radical scavenging activity was expressed as percentage according to the
following equation:
Where, A1 is the absorbance of samples, and
A0 is the absorbance of control (distilled
water).
9
Quantifying Total Flavonoid Content
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Total flavonoid content was measured as described who applied the Davis method with modifications26 . To 100 μL of sample solution, 1 mL of 90% diethylene glycol and 100 μL of 1 N NaOH were added, and the mixture was shaken and allowed to stand in a water bath (SB-1200, Eyela, Tokyo, Japan) at 37 °C for 1 h. The absorbance was measured at 420 nm using the spectrophotometer. Standard curve was plotted using quercetin as the reference, and total flavonoid content was expressed as mg quercetin equivalents per 100 g sample (mg QE/100 g).
10
Lipid Extraction from Coffee Grounds
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The equivalent of a cup of coffee SCGs, 10 g was gently stirred in 100 mL of n-hexane at room temperature for 1 day. The solvent was removed by reduced pressure evaporation at 80°C (EYELA, SB-1200). Approximately about 70 µl of lipids were extracted as a clear yellow brown oil in liquid form, and solid residues were filtered (Figure 1). The extraction rate of TAG was estimated by comparing integrated intensity ratio of the signal at 2.6~2.7 ppm in 1 H-MAS NMR spectra. (Figure 3 (b) and Figure 4 (b)) 1
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