Log In Sign up
The largest database of trusted experimental protocols

Gibco branded cell culture products

Manufactured by Thermo Fisher Scientific
Visit Supplier

Gibco-branded cell culture products are a comprehensive range of reagents, media, and supplements designed to support the growth and maintenance of various cell lines in laboratory environments. These products are formulated to provide optimal conditions for cell culture, enabling researchers to maintain and propagate cells effectively. The core function of Gibco-branded cell culture products is to support the in vitro cultivation of diverse cell types, facilitating research and development in fields such as cell biology, biotechnology, and drug discovery.

Automatically generated - may contain errors

Lab products found in correlation

Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (4 mentions) Succinylacetone, by Merck Group (2 mentions) Mannitol, by Merck Group (2 mentions) Isoproterenol, by Merck Group (2 mentions) 8 br camp, by Merck Group (2 mentions) Protoporphyrin 9, by Merck Group (2 mentions) Cl 316 243, by Cayman Chemical (2 mentions) Forskolin, by Chem-Impex (2 mentions) Insulin novolin, by Novo Nordisk (2 mentions) On target sirna smartpools, by Horizon Discovery (2 mentions) Hemin, by Merck Group (2 mentions) Hek293t cell, by American Type Culture Collection (2 mentions) Oligomycin a, by Merck Group (2 mentions) Antimycin a, by Merck Group (2 mentions) Norepinephrine, by Merck Group (2 mentions) Complete edta free protease inhibitor cocktail, by Roche (2 mentions)

Spelling variants of this product

Cell culture products (31 citations)

2 protocols using gibco branded cell culture products

1

Heme Regulation of Cellular Metabolism

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Hemin, protoporphyrin IX, oligomycin A, carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone (FCCP), rotenone, antimycin A, 3-isobutyl-1-methylxanthine (IBMX), BSA, mannitol, norepinephrine, isoproterenol, 8-Br-cAMP, and succinylacetone were purchased from Sigma-Aldrich. CL 316,243 was obtained from Cayman Chemical. Forskolin was obtained from Chem Impex International. Insulin (Novolin) was purchased from Novo-Nordisk. Complete EDTA-free protease inhibitor cocktail was obtained from Roche. DMEM and other Gibco-branded cell culture products were purchased from Thermo Fisher. Heme-depleted FBS was prepared by treating FBS with 20 mM ascorbic acid for 16 hr, followed by 24 hr dialysis against PBS. Heme depletion was verified by measuring optical absorbance at 405 nm. CPAG-1 was synthesized as previously reported5 (link). ON-TARGET siRNA SMARTpools against human PGRMC2 (L-010639-00-0005), PGRMC1 (L-010642-00-0005), and mouse Nr1d1 (L-051721-00-0005), and BACH1 (L-042956-01-0005), as well as a Non-targeting Pool (D-001810-10-05) were purchased from Dharmacon. HEK293T cells were obtained from ATCC (CRL-3216) having undergone short-tandem repeat verification. Cells were routinely tested for mycoplasma and were never positive.
Galmozzi A., Kok B.P., Kim A.S., Montenegro-Burke J.R., Lee J.Y., Spreafico R., Mosure S., Albert V., Cintron-Colon R., Godio C., Webb W.R., Conti B., Solt L.A., Kojetin D., Parker C.G., Peluso J.J., Pru J.K., Siuzdak G., Cravatt B.F, & Saez E. (2019). PGRMC2 is an Intracellular Heme Chaperone Critical for Adipocyte Function. Nature, 576(7785), 138-142.
+ Open protocol
+ Expand
2

Heme Regulation of Cellular Metabolism

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Hemin, protoporphyrin IX, oligomycin A, carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone (FCCP), rotenone, antimycin A, 3-isobutyl-1-methylxanthine (IBMX), BSA, mannitol, norepinephrine, isoproterenol, 8-Br-cAMP, and succinylacetone were purchased from Sigma-Aldrich. CL 316,243 was obtained from Cayman Chemical. Forskolin was obtained from Chem Impex International. Insulin (Novolin) was purchased from Novo-Nordisk. Complete EDTA-free protease inhibitor cocktail was obtained from Roche. DMEM and other Gibco-branded cell culture products were purchased from Thermo Fisher. Heme-depleted FBS was prepared by treating FBS with 20 mM ascorbic acid for 16 hr, followed by 24 hr dialysis against PBS. Heme depletion was verified by measuring optical absorbance at 405 nm. CPAG-1 was synthesized as previously reported5 (link). ON-TARGET siRNA SMARTpools against human PGRMC2 (L-010639-00-0005), PGRMC1 (L-010642-00-0005), and mouse Nr1d1 (L-051721-00-0005), and BACH1 (L-042956-01-0005), as well as a Non-targeting Pool (D-001810-10-05) were purchased from Dharmacon. HEK293T cells were obtained from ATCC (CRL-3216) having undergone short-tandem repeat verification. Cells were routinely tested for mycoplasma and were never positive.
Galmozzi A., Kok B.P., Kim A.S., Montenegro-Burke J.R., Lee J.Y., Spreafico R., Mosure S., Albert V., Cintron-Colon R., Godio C., Webb W.R., Conti B., Solt L.A., Kojetin D., Parker C.G., Peluso J.J., Pru J.K., Siuzdak G., Cravatt B.F, & Saez E. (2019). PGRMC2 is an Intracellular Heme Chaperone Critical for Adipocyte Function. Nature, 576(7785), 138-142.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!