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Apc conjugated anti annexin 5 apoptosis detection kit 1

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The APC-conjugated anti–Annexin V Apoptosis Detection kit is a laboratory reagent used for the detection and quantification of apoptosis in cell samples. It contains Annexin V, a protein that binds to phosphatidylserine, a biomarker for apoptosis, which is conjugated to the fluorescent dye allophycocyanin (APC).

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Annexin V-APC Apoptosis Detection Kit I APC Annexin V Apoptosis Detection Kit Annexin V Apoptosis Detection Kit I Annexin V-APC Apoptosis Kit Annexin V Apoptosis Detection Kit APC-conjugated Annexin V (Annexin V-APC) kit APC-conjugated Annexin V Annexin V Apoptosis Kit APC Annexin V kit Anti-Annexin V-APC

2 protocols using apc conjugated anti annexin 5 apoptosis detection kit 1

1

Quantifying Apoptosis in APC Tumor Models

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Apoptotic cell death was measured by APC-conjugated anti–Annexin V Apoptosis Detection kit I (BD). Apoptotic cell death in tumor tissue lysates of Apcmin/+, Apcmin/+Pld1Tg, Apcmin/+Pld1−/−, and Apcmin/+Pld1−/−Pld1Tg mice (18 wk; n = 5–8 for each group) was measured by use of a colorimetric assay (Caspase-3 kit; BioVision) according to the manufacturer’s instructions.
Kang D.W., Choi C.Y., Cho Y.H., Tian H., Di Paolo G., Choi K.Y, & Min D.S. (2015). Targeting phospholipase D1 attenuates intestinal tumorigenesis by controlling β-catenin signaling in cancer-initiating cells. The Journal of Experimental Medicine, 212(8), 1219-1237.
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2

Quantifying Apoptotic Cell Death

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Apoptotic cell death was measured by APC‐conjugated anti‐Annexin V Apoptosis Detection Kit I (550474; BD Bioscience). The terminal deoxynucleotidyl transferase dUTP nick‐end labeling (TUNEL) assay was performed using In Situ Cell Death Detection Kit, POD (Roche), according to the manufacturer's protocol.
Kang D.W., Hwang W.C., Noh Y.N., Kang Y., Jang Y., Kim J, & Min D.S. (2020). Phospholipase D1 is upregulated by vorinostat and confers resistance to vorinostat in glioblastoma. Journal of Cellular Physiology, 236(1), 549-560.
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