Agilent 5973 inert mass selective detector

Manufactured by Agilent Technologies

Sourced in United States

The Agilent 5973 Inert Mass Selective Detector is a high-performance mass spectrometer designed for analytical laboratories. It provides accurate and reliable mass analysis of chemical compounds. The detector features an inert ion source that minimizes analyte adsorption, ensuring precise and reproducible results.

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Lab products found in correlation

6890n network gas chromatograph, by Agilent Technologies (2 mentions) Agilent 6890n network gc system, by Agilent Technologies (1 mentions) Lc 50, by J&K Scientific (1 mentions) Db 5 gc column, by Agilent Technologies (1 mentions) Varian 9012 solvent delivery system, by Agilent Technologies (1 mentions) Db 5ms, by Agilent Technologies (1 mentions)

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5973 Mass Selective Detector (53 citations) Agilent 5973 (43 citations) Agilent 5973 Mass Selective Detector (13 citations) 5973 inert mass selective detector (7 citations) 5973 inert (6 citations)

4 protocols using agilent 5973 inert mass selective detector

GC-MS Quantification of Urinary Allantoin

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Allantoin was measured in the urine by the adaptation of the method developed by Gruber [15 (link), 13 ]. In summary, 25 µL of urine was spiked with 400 µL of 10 µM internal standard (DL-allantoin- 5-¹³C;1-¹⁵N). The spiked samples were simultaneously deproteinized and extracted with 100 µL acetonitrile, vortexed and centrifuged at 20 000 g, 4^oC, for 5 minutes. The supernatant was dried using the speed vacuum drier and derivatized with 50 µL of MTBSTFA (i.e. N-methyl-N-tert-butyl-dimethyl-silyltrifluoroacetamide) in pyridine (1:1 vol/vol). The derivatization process was facilitated by incubation at 50^oC for 2 hours. Analysis was performed on Agilent 6890N Network GC System connected to an Agilent 5973 Inert Mass Selective Detector (both Agilent Technologies, Inc, Santa Clara, California). Separation was performed using an Agilent 122-5532G capillary column (25.7 m length, 0.25 mm internal diameter). Allantoin was quantified using selected ion monitoring mode with the 398.00 m/z ion being monitored for allantoin and the 400.00 m/z for DL-allantoin- 5-¹³C;1-¹⁵N. The ion abundance ratios (398.00/ 400.00) were converted to micromolar concentrations by use of a standard curve.

Esiaba I., Angeles D.M., Holden M.S., Tan J.B., Asmerom Y., Gollin G, & Boskovic D.S. (2015). Urinary allantoin is elevated in severe intraventricular hemorrhage in the preterm newborn. Translational stroke research, 7(2), 97-102.

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GC/MS Analysis of Polycyclic Aromatic Hydrocarbons

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GC/MS analyses were performed on an Agilent 6890N Network Gas Chromatograph coupled to an Agilent 5973 Inert Mass Selective Detector (Agilent, Santa Clara, CA, USA) using the following GC columns: (i) a Restek Rxi-PAH, later described as 50 % diphenyl – 50 % dimethylpolysiloxane - like phase (Restek, Bellefonte, PA), 60 m length, 0.25 mm id, 0.10 μm film thickness, and maximum programmable temperature 360 °C; (ii) an Agilent DB-5 with 5 % diphenyl – 95 % dimethylpolysiloxane (Agilent, Santa Clara, CA, USA), 60 m length, 0.25 mm id, 0.25 μm film thickness, and maximum programmable temperature 350 °C; (iii) a SE-52 with 5 % diphenyl – 95 % dimethylpolysiloxane of 12 m length, 0.29 mm id, and 0.34 μm film thickness whose operating parameters are provided in reference 19 ; (iv) a J&K Scientific LC-50 column (50 % liquid crystal) with dimethylpolysiloxane stationary phase (J&K Scientific, Edwardsville, Nova Scotia, Canada), 20 m length, 0.25 mm id, 0.10 μm film thickness, and 270 °C maximum temperature; and (v), a J&K Scientific LC-50 of 15 m length whose column dimensions and operating parameters are provided in reference 17 (link).

Oña-Ruales J.O., Wilson W.B., Nalin F., Sander L.C., Schubert-Ullrich P, & Wise S.A. (2016). The Influence of the Aromatic Character in the Gas Chromatography Elution Order: The Case of Polycyclic Aromatic Hydrocarbons. Molecular physics, 114(23), 3533-3545.

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NPLC/UV-Vis and GC/MS Analyses

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NPLC/UV-Vis was performed using a Varian 9012 Solvent Delivery System (Agilent, Santa Clara, CA) coupled to Jasco UV-1570 Intelligent UV/Vis Detector (Jasco, Easton, MD) using a Waters Spherisorb 5 μm NH₂ 10 × 250 mm semi-prep LC column (Waters, Milford, MA). GC/MS analyses were performed on an Agilent 6890N Network Gas Chromatograph coupled to Agilent 5973 Inert Mass Selective Detector (Agilent, Santa Clara, CA) using a Restek Rxi-PAH GC Column (Restek, Bellefonte, PA), 60 m length, 0.25 mm id, 0.10 μm film thickness, maximum programmable temperature 360 °C, and minimum bleed at 350 °C, and using an Agilent DB-5 GC Column (Agilent, Santa Clara, CA), 5% phenylmethylpolysiloxane stationary phase, 60 m length, 0.25 mm id, 0.25 μm film thickness, maximum programmable temperature 350 °C, and minimum bleed at 325 °C.

Oña-Ruales J.O., Sharma A.K, & Wise S.A. (2015). Identification and Quantification of Six-Ring C26H16 Cata-Condensed Polycyclic Aromatic Hydrocarbons in a Complex Mixture of Polycyclic Aromatic Hydrocarbons from Coal Tar. Analytical and bioanalytical chemistry, 407(30), 9165-9176.

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Quantification of Polycyclic Aromatic Compounds by GC-MS

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Sixteen PAHs (US EPA PAHs), 12 O-PACs, 5 N-PACs and 4 S-PACs were quantified by Gas Chromatography coupled to a Mass Spectrometer (GC-MS) using internal deuterated standards (naphthalene D8, acenaphthene D10, phenanthrene D10, pyrene D10, chrysene D12, perylene D12, benzo(ghi)perylene, quinoline D7, 9H fluorenone D8). Two calibration curves were drawn for low (0, 0.06, 0.12, 0.18, 0.3 and 0.6 µg/mL), and high concentrations (0.18, 0.3, 0.6, 1.2, 3, 6 and 9.6 µg/mL).
The instrument used was a GC Agilent Technology 6890N equipped with a column DB-5MS (60 m × 0,25 mm d.i. × 0,25 µm film thickness -Agilent Tech.) coupled to an Agilent 5973 inert Mass Selective
Detector. One microliter of sample was injected at 300 °C in splitless mode. The GC oven temperature was programmed from 70 °C (held 2 min) to 130 °C at 15 °C/min, then from 130 to 315 °C (held 30 min) at 4 °C/min. The carrier gas was helium at 1.6 ml/min at constant flow.
Three analyses were carried out on each EOM sample: (i) quantitative analysis in Single Ion Monitoring (SIM) mode (Online Resources Table ESM_1), to lower the detection limit for all targeted molecules, (ii) qualitative analysis in Scan mode, and (iii) qualitative analysis (Scan mode) of the EOM previously derivatized (BSTFA-TMCS (99:1 Sylon BFT, Supelco)/sample (50/50 v/v) heated at 60 °C for 30 min (Wenclawiak et al., 1993) ) to detect acids and alcohols.

Johansson C., Bataillard P., Biache C., Lorgeoux C., Colombano S., Joubert A., Pigot T, & Faure P. (2020). Ferrate^VI oxidation of polycyclic aromatic compounds (PAHs and polar PACs) on DNAPL-spiked sand: degradation efficiency and oxygenated by-product formation compared to conventional oxidants. Environmental science and pollution research international, 27(1).

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