Female Sprague Dawley rats (Janvier, Saint-Berthevin, France) weighing 250–300 g were used for in situ hybridization, immunohistofluorescence, immunoprecipitation, Western blotting of tissue explants, electron microscopy, and intracerebral infusion experiments. Vaginal smears were examined daily, and only rats that exhibited at least two consecutive 4-d estrous cycles were used for experiments. Diestrus 1 and 2 were defined by the predominance of leukocytes in the vaginal lavage, the day of proestrus was characterized by the predominance of round nucleated epithelial cells, and estrus was distinguished by a large number of clustered cornified squamous epithelial cells.
Female sprague dawley rats
Manufactured by Janvier Labs
Sourced in France
Female Sprague-Dawley rats are laboratory animals commonly used in research and testing. They are a breed of albino rats that are known for their docile temperament and reliable breeding characteristics. These rats are primarily utilized for studying various biological and medical processes.
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8 protocols using female sprague dawley rats
1
Sprague Dawley Rat Estrous Cycle
2
Female Rat Pharmacokinetic Study
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All animal experiments were performed in accordance with the Animal Welfare Act and the guidelines of the North Rhine-Westphalia State Environment Agency (Landesamt für Natur, Umwelt und Verbraucherschutz). Female Sprague–Dawley rats (Janvier Labs) weighing between 220 and 250 g were used throughout the study. A total of 194 rats were used in this study including those used for the PK study. The animals were housed three rats per cage with room temperature controlled at 21–22°C, and an artificial 12 h light/dark cycle. Rats were given food and water ad libitum throughout the experiment. Methods and results are written in accordance with the ARRIVE (Animal Research: Reporting of In Vivo Experiments) guidelines for publishing in vivo research.29
3
Sprague-Dawley Rat Maintenance and Handling
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Female Sprague-Dawley rats (8–9 weeks old, ca. 200 g, Janvier Labs, Saint-Berthevin, France) were used. The animals were maintained and handled in accordance with the Guidelines for Accommodation and Care of Animals (European Convention for the Protection of Vertebrate Animals Used for Experimental and Other Scientific Purposes) and internal guidelines. All experimental procedures were approved by the ethical committee of CIC biomaGUNE and local authorities before conducting experimental work. All animals were housed in ventilated cages and fed on a standard diet ad libitum.
4
Sprague-Dawley Rat Acclimation Study
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All the surgical and experimental procedures were designed, approved, and performed in accordance with the EU directive for the use of animals in research (2010/63/EU), approved by the local ethical committee and registered with the Swedish Department of Agriculture (Jordbruksverket). Female Sprague-Dawley rats (Janvier–Germany) were kept under a 12:12 hours light/dark cycle, constant temperature of 21° C, 50% humidity and ad libitum access to water and food. The rats were acclimatized to the new environment for 5 days before any experimental procedure.
5
Spinal Cord Transection in Sprague-Dawley Rats
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Female Sprague-Dawley rats (Janvier Laboratories, Le Genest Saint Isle, France), 9 weeks of age and weighting 280 g were housed for 6–7 days in their definitive animal facility, in a controlled environment with free access to food and water before experiments. The experiments were carried out in strict accordance with the European Communities Council Directives 2010/63/UE on the use of laboratory animal and care regulation in force in France (Ministry of Agriculture, Authorization Agreement No. A78-322-3, December 2013 and B78-423-1, July 2017) on a total of nine control (CT) rats and nine spinalized rats, 21 days after they had undergone a T8–9 spinal cord transection.
6
Sprague-Dawley Rat Neuroscience Experiments
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All the animal procedures were approved by the Danish Animal Experiments Inspectorate under the Danish Ministry of Environment and Food, license 2015-15-0201-00535, apart from the behavioral experiments, which were approved by the County Administrative Board of Southern Finland, license ESAVI/9697/04.10.07/2017. Female Sprague-Dawley rats (10-13 weeks, 175–250 g) acquired from Janvier were used in the experiments. Rats were group-housed in 12:12h light cycle with access to food and water ad libitum. All efforts were made to minimize pain or discomfort of the animals used. The well-being of the animals was monitored daily, indicated by grooming behavior and body posture. After implantation of a guide cannula in the experiments that involved striatal infusions, the rats were moved to single housing overnight to prevent detachment of the cannula. All experiments were performed at Zeitgeber time 2-10.
7
Sprague-Dawley Rat Tissue Transplant
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Female Sprague-Dawley rats (Janvier Labs, Le Genest-Saint-Isle, France) were housed at 12-h light–dark cycle with food and water ad libitum. For the preparation of the transplants, pregnant Sprague-Dawley rats were purchased from Janvier Labs. All experiments were carried out in the light phase and in accordance with the guidelines of the Animal Research Ethics Committee of the Canton Berne, Switzerland, and the University of Bern Animal Care and Use Committee, Switzerland.
8
Sprague Dawley Rat Housing Protocol
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Female Sprague Dawley rats (Janvier, France) were purchased at 10 weeks old and used at body weight of approx. 300 g. The animals were housed in individually ventilated cages and provided with clean food (RM1(E), SDS, UK) and sterile water ad libitum. The housing conditions were free of specific pathogens according to recommendations from the Federation of European Laboratory Animal Science Association, with controlled temperatures between 19 and 22 °C, relative humidity between 50 and 60% and 12 h light and dark cycles. All animals were acclimatised for one week before starting the experiment. All experimental procedures involving laboratory animals were performed in compliance with protocols approved by the Norwegian Food Safety Authorities, according to EU directive 2010/63/EU on the protection of animals used for scientific purposes, and in accordance with the 3 R's principle.
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