Pab10348

Manufactured by Abnova

PAB10348 is a laboratory equipment product. It is designed for specific functions within a laboratory setting. No further details about its core function or intended use are provided, as this would require interpretation or extrapolation beyond the factual and unbiased information available.

Automatically generated - may contain errors

Lab products found in correlation

Hsp70, by Santa Cruz Biotechnology (1 mentions) C myc, by Santa Cruz Biotechnology (1 mentions) Pvdf membrane, by Merck Group (1 mentions)

2 protocols using pab10348

Western Blot Analysis of Protein Interactions

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Cells were lysed in RIPA lysis buffer (50 mM Tris–HCl, 150 mM NaCl, 5 mM EDTA, 0.5% Nonidet P-40 and a protease and phosphatase inhibitor cocktail (Calbiochem)). Proteins were separated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to 0.45 μM PVDF membranes (Millipore). The immunoblots were processed according to standard procedures using primary antibodies directed to p300 (BD, 554215), CBP (CST, 4772), GAPDH (CST, 2118), HA (CST, 3724), Flag (CST, 14793), Hsp70 (Santa Cruz, sc-24), p53 (Santa Cruz, sc-6243), c-Myc (Santa Cruz, sc-40), hSSB1 (Bethyl, A301–938A), NBS1 (Bethyl, A300–187), tubulin (Bioworld, BS1482M) and ac-K (abnova, PAB10348).

Wu Y., Chen H., Lu J., Zhang M., Zhang R., Duan T., Wang X., Huang J, & Kang T. (2015). Acetylation-dependent function of human single-stranded DNA binding protein 1. Nucleic Acids Research, 43(16), 7878-7887.

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Antibody Characterization for Protein Modifications

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Antibodies used in this study were as follows: Flag (D6W5B) Rabbit mAb (CST, 8146S), HA-Tag (C29F4) Rabbit mAb (CST, 3724), anti-Acetylated-Lysine Antibody (CST, 9441s), Acetylated lysine polyclonal antibody (PAB10348, abnova), RhoA (67B9) Rabbit mAb (CST, #2117S), β-Tubulin (9F3) Rabbit mAb (CST,#2128), GAPDH (D16H11) Rabbit mAb (CST, #5174), SirT1 (D1D7) Rabbit mAb (CST, #9475) ,HDAC6(D2E5) Rabbit mAb (CST, #7558), BD Pharmingen™ Purified Mouse Anti-Human p300 (BD, 554215), CBP (D6C5) Rabbit mAb (CST, #7389). mAb RAD5-8 and hRAD5-8-v1-R5 antibody were generated as previously described 8 .
The specific Anti-K7ac-Rab22a-NeoF1 antibody is designed and generated by ptm-biolab according to the following peptides table 1:

Liang X., Wang X., He Y., Wu Y., Zhong L., Liu W., Liao D, & Kang T. (2020). Acetylation dependent functions of Rab22a-NeoF1 Fusion Protein in Osteosarcoma. Theranostics, 10(17), 7747-7757.

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