hPDMS premix composition is as follows: 3.4 g of VDT-731 (Gelest, Inc., Cat#VDT-731), 18 μL of Pt catalyst (Platinum(0)-2,4,6,8-tetramethyl-2,4,6,8-tetravinylcyclotetrasiloxane complex solution) (Sigma-Aldrich, Cat#479543), 5 μL of cross-linking modulator 2,4,6,8-Tetramethyl-2,4,6,8-tetravinylcyclotetrasiloxane (Sigma-Aldrich, Cat#396281). The resulting mixture was mixed in a 50 mL conical bottom centrifuge tube on the vortex mixer for at least 30 sec, then 1 g of HMS-301 (Gelest, Inc., Cat#HMS-301) was added immediately before use and mixed for 30 sec on a vortex mixer 78 ,80 .
Hms 301
Manufactured by Gelest
Sourced in United States
The HMS-301 is a laboratory equipment designed for heating and stirring sample mixtures. It features a digital display for temperature and stirring speed control. The core function of the HMS-301 is to provide consistent and reliable heating and mixing of samples in a laboratory setting.
Automatically generated - may contain errors
Lab products found in correlation
Vdt 731, by Gelest (19 mentions)
Sip6831, by Gelest (8 mentions)
Sylgard 184a, by Dow (4 mentions)
Sylgard 184b, by Dow (4 mentions)
A collagen 1 rabbit pab, by Abcam (2 mentions)
Cross linking modulator, by Merck Group (2 mentions)
Dms v31, by Gelest (2 mentions)
2 4 6 8 tetramethyl 2 4 6 8 tetravinylcyclotetrasiloxane, by Merck Group (2 mentions)
Sit8174, by Gelest (2 mentions)
Sit7900, by Gelest (2 mentions)
Tween 60, by Merck Group (1 mentions)
Cab o sil ts 720, by Cabot (1 mentions)
Hfbma, by Apollo Scientific (1 mentions)
Silicon dioxide amorphous hexamethyldisilazane treated particles sis6962.0, by Fluorochem (1 mentions)
Potassium hydroxide, by Merck Group (1 mentions)
Dimethylformamide dmf, by Merck Group (1 mentions)
Dipentaerythritol penta hexa acrylate, by Merck Group (1 mentions)
1 hydroxycyclohexyl phenyl ketone, by Merck Group (1 mentions)
Sylgard 184 silicone elastomer kit, by Dow (1 mentions)
Toluene, by Merck Group (1 mentions)
25 protocols using hms 301
1
Silicone Elastomer Premix Composition
2
Preparation of hPDMS Polymer Mixture
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To prepare the hPDMS mixture we used 3.4 g of VDT-731 (Gelest, Inc., Cat# VDT-731), 18 μL of Pt catalyst (Platinum(0)-2,4,6,8-tetramethyl-2,4,6,8-tetravinylcyclotetrasiloxane complex solution) (Sigma-Aldrich, Cat# 479543), and one drop of cross-linking modulator 2,4,6,8-Tetramethyl-2,4,6,8 -tetravinylcyclotetrasiloxane (Sigma-Aldrich, Cat# 396281). All components were thoroughly mixed in a 50 mL conical bottom centrifuge tube on the vortex mixer for at least 30 sec. Before use, we added 1 g of HMS-301 (Gelest, Inc., Cat# HMS-301) to the mixture, promptly mixed it for 30 sec on a vortex mixer, degassed in a high-speed centrifuge (3 minutes, 3000 rpm), and immediately used it for the mold coating. The detailed protocol is described elsewhere (74 , 76 ).
3
Preparation of Crosslinked hPDMS Mixture
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Briefly, the hPDMS mixture was prepared
as follows: 3.4 g of VDT-731 (Gelest, Inc., Cat#VDT-731), 18 μL
of Pt catalyst (Platinum(0)-2,4,6,8-tetramethyl-2,4,6,8-tetravinylcyclotetrasiloxane
complex solution) (Sigma-Aldrich, Cat#479543), and one drop of cross-linking
modulator 2,4,6,8-tetramethyl-2,4,6,8-tetravinylcyclotetrasiloxane
(Sigma-Aldrich, Cat#396281) were thoroughly mixed in a 50 mL conical
bottom centrifuge tube on the vortex mixer for at least 30 s. Lastly,
immediately before use, we added to the mixture 1 g of HMS-301 (Gelest,
Inc., Cat#HMS-301), promptly mixed it for 30 s on a vortex mixer,
and immediately used it for the mold coating. A detailed hard PDMS
(hPDMS) formulation and related protocols are described elsewhere.107 (link),108 (link)
as follows: 3.4 g of VDT-731 (Gelest, Inc., Cat#VDT-731), 18 μL
of Pt catalyst (Platinum(0)-2,4,6,8-tetramethyl-2,4,6,8-tetravinylcyclotetrasiloxane
complex solution) (Sigma-Aldrich, Cat#479543), and one drop of cross-linking
modulator 2,4,6,8-tetramethyl-2,4,6,8-tetravinylcyclotetrasiloxane
(Sigma-Aldrich, Cat#396281) were thoroughly mixed in a 50 mL conical
bottom centrifuge tube on the vortex mixer for at least 30 s. Lastly,
immediately before use, we added to the mixture 1 g of HMS-301 (Gelest,
Inc., Cat#HMS-301), promptly mixed it for 30 s on a vortex mixer,
and immediately used it for the mold coating. A detailed hard PDMS
(hPDMS) formulation and related protocols are described elsewhere.107 (link),108 (link)
4
Preparation of Hard PDMS Mixture
5
Fabrication of Elastic Collagen Nano- and Micro-Patterns
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Fabrication of elastic collagen nano- and micro-patterns is a challenging task due to the susceptibility of type-I collagen to undergo rapid gelation, and van-der-waals and capillary interactions between the nano-stamp and the printed surface that provoke a collapse of the soft PDMS nano-stamps onto the glass surface. To address these issues and achieve high precision micro- and nano-patterns on elastic platforms we (i) substituted regular PDMS nano-stamps with composite stamps, veneered with a submillimeter-thick hard PDMS (hPDMS) for non-collapsing high-definition printing surfaces (Schmid and Michel, 2000 ; Tabdanov et al., 2015 ), and (ii) substituted collagen with a-collagen-1 rabbit pAb (AbCam, Cambridge, UK), conjugated with biotin and a fluorescent tag, to ensure cross-linking of the antibody to PAA gels and for fluorescence visibility, respectively. For hPDMS we mixed 3.4g of VDT-731 (Gelest, Inc.), 18mL of Pt catalyst (Platinum(0)-2,4,6,8-tetramethyl-2,4,6,8-tetravinylcyclotetrasiloxane complex solution) (Sigma-Aldrich) and one drop of cross-linking modulator (2,4,6,8-Tetramethyl-2,4,6,8-tetravinylcyclotetrasiloxane) (Sigma-Aldrich). Next, immediately before use, we added 1g of HMS-301 (Gelest, Inc.) and thoroughly mixed it for 30sec on vortex mixer (Odom et al., 2002 ).
6
Fabrication of Elastic Collagen Nano- and Micro-Patterns
7
Siloxane-based Ink Formulations
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The inks created in this study consist of a siloxane and fumed silica base similar to that of Brounstein et al. [19 (link)]. Siloxanes used in this study include vinyl-terminated (4–6% diphenylsiloxane)-dimethylsiloxane copolymer (Gelest PDV-541) and trimethylsiloxy-terminated methylhydrosiloxane-dimethylsiloxane copolymer (Gelest HMS-301) (Gelest, Inc., Morrisville, PA, USA). To prevent premature curing of the inks, we used 1-ethynyl-1-cyclohexanol (ETCH; 99%, Sigma Aldrich) (Millipore Sigma, St. Louis, MO, USA). A high-temperature platinum catalyst (Gelest SIP 6829.2; platinum carbonyl cyclovinylmethylsiloxane complex; 1.85–2.1% Pt in cyclomethyl vinyl siloxanes) and a mid-temperature platinum catalyst (Gelest SIP 6832.2; platinum-cyclovinylmethyl-siloxane complex; 2% Pt in cyclomethylvinylsiloxanes) were used to induce crosslinking. Fillers included an OH-functionalized fumed silica (Evonik Aerosil 300, Evonik Industries AG, Essen, Germany), a PDMS-functionalized silica (CAB-O-SIL TS-720; Cabot Corporation, Boston, MA, USA), powdered tungsten metal (99.9%, American Elements and 99.9%, Sigma Aldrich) (American Elements, Los Angeles, CA, USA), powdered tungsten oxide (99.9%, American Elements and ≥99%, Sigma Aldrich), powdered gadolinium oxide (99.9%; American Elements), and a surfactant (TWEEN 60, Sigma Aldrich).
8
Facile Fabrication of Hydrophobic Nanostructures
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Before replicating the nanostructures, the fabricated master mold was treated with an O2 plasma process to decontaminate it and improve hydrophilicity. The surface‐treated master mold formed a self‐assembled monolayer via a vaporized silane coupling agent (Trichloro‐1H, 1H, 2H, 2H‐Perfluorooctyl‐silane, Sigma–Aldrich) coating for 5 min at 130 °C, thereby exhibiting a hydrophobic surface, which allowed for facilitated demolding. After the surface treatment of the master mold, h‐PDMS was prepared by blending 1.7 g of vinylmethyl copolymer (VDT731, Gelest), 9 µL of platinum‐catalyst (SIP6831.2, Gelest), 0.05 g of the modulator (2,4,6,8 – tetramethyl‐ 2,4,6,8 – tetravinylcyclotetrasiloxane, Sigma–Aldrich), 1 g of toluene, and 0.5 g of siloxane‐based silane reducing agent (HMS‐301, Gelest). The h‐PDMS was spin coated on the master‐mold at 2,000 rpm for 60 s, followed by a baking process at 70 °C for 2 h to harden the h‐PDMS. Then, the PDMS mixture consisting of a 1:10 weight ratio of PDMS (Sylgard 184 A, Dow corning) and curing agent (Sylgard 184 B, Dow corning) was poured onto the h‐PDMS layer, followed by baking at 70 °C for 2 h to harden the h‐PDMS. After this process, the hardened soft mold was demolded from the master mold and used for replication.
9
Fabrication of PDMS-HFBMA Bicontin uious Samples
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First, we prepare pure PDMS matrices by mixing PDMS chains (DMS-V31, Gelest) with a crosslinker (HMS-301, Gelest) and a platinum-based catalyst (SIP6831.2, Gelest) (see full recipe in [47] ). The stiffness of the matrix depends on the mass ratio between the chains and crosslinker (from 3:1 to 9:1), while keeping the catalyst concentration constant (0.0019% in volume). Once the different parts are thoroughly mixed together, we pour the mixture into a petri dish, degassed it in vacuum, and finally cure it at 60 • C for approximately 6 days. After curing, the resulting PDMS elastomer is carefully removed from the petri dish and cut into rectangular pieces (∼1cm×2cm×0.5cm).
Next, PDMS pieces are transferred into a bath of heptafluorobutyl methacrylate (HFBMA, Apollo scientific) (∼1mL HFBMA/0.5g of PDMS), in a 25mL glass bottle. This bath is then typically incubated at T swell = 60 • C in a pre-heated oven during 2.5 days. After the elastomer is saturated with the liquid, the glass bottle is brought to room temperature, at which point phase separation occurs spontaneously. The resulting phase-separated bicontiunous samples are then prepared for characterization tests.
Note that experiments with un-crosslinked PDMS are performed by preparing mixtures of PDMS chains and HFBMA liquid in glass bottles, and following the same temperature steps described above.
Next, PDMS pieces are transferred into a bath of heptafluorobutyl methacrylate (HFBMA, Apollo scientific) (∼1mL HFBMA/0.5g of PDMS), in a 25mL glass bottle. This bath is then typically incubated at T swell = 60 • C in a pre-heated oven during 2.5 days. After the elastomer is saturated with the liquid, the glass bottle is brought to room temperature, at which point phase separation occurs spontaneously. The resulting phase-separated bicontiunous samples are then prepared for characterization tests.
Note that experiments with un-crosslinked PDMS are performed by preparing mixtures of PDMS chains and HFBMA liquid in glass bottles, and following the same temperature steps described above.
10
Hierarchical PDMS Replication from HSQ Molds
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Hard PDMS (hPDMS) templates were replicated from the patterned HSQ molds. Oxygen plasma-treated molds were functionalized with 1H,1H,2H,2H-perfluorodecyltriethoxysilane (MilliporeSigma) to prevent adhesion to the PDMS template. Prepolymer mixture of hPDMS, consisting of 3.4-g VDT-731 (vinylmethylsiloxane-dimethylsiloxane, Gelest), 18 μl of SIP6831.2 (platinum catalyst, Gelest), 5 μl of 2,4,6,8-tetramethyl-2,4,6,8-tetravinylcyclotetrasiloxane (MilliporeSigma), and 1.0-g HMS-301 (methylhydrosiloxane-dimethylsiloxane copolymer, Gelest) (40 (link)), was thoroughly mixed. The mixture was immediately spin-coated onto the fluorinated mold at 3000 rpm for 30 s. The mold was then brought into contact with a soft PDMS (Sylgard 184) film on a glass backing. The stack was cured at 75°C for 14 hours. Once cured, the PDMS was peeled from the mold exposing the topographical template.
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