Sabc immunohistochemistry staining kit

All the tooth samples and the kidneys containing tooth germs were performed for hematoxylin & eosin staining and AZON staining, respectively. The sABC immunohistochemistry staining kit (Boster, Wuhan, China) was used for the immunohistochemical staining or immunocytochemical staining. Antibodies included mouse monoclonal IgG anti-DSP (kindly provided by Dr. Rong Zhang from Fourth Military Medical University), mouse monoclonal anti-vimentin antibody, mouse monoclonal anti-pan cytokeratin antibody (Abcam, UK).

Ang II was obtained from MedChemExpress (#HY-B0202; Princeton, NJ, USA). The primary antibodies against KDM1A (BM4356), ALCAM (A01788-1), MBNL1 (A02309-1), and TSN (A02777) were purchased from Boster Biological Technology (Wuhan, Hubei, China). Anti-TMEM41B (29270-1-AP), -PRTN3 (25278-1-AP), -GIGYF2 (24790-1-AP), -NHP2 (15128-1-AP), -RDH10 (14644-1-AP), and -TIMD4 (12008-1-AP) antibodies were purchased from Proteintech (Chicago, IL, USA). Anti-CtBP1 (ab129181), -PKN1 (ab231038), and -CCN1 (ab228592) antibodies were purchased from Abcam (Cambridge, MA, USA). Peroxidase-conjugated AffiniPure goat anti-rabbit IgG (H + L) was from Proteintech (SA00001–2; Chicago, IL, USA). An SABC immunohistochemistry staining kit was sourced from BosterBio (SA1028; Wuhan, Hubei, China). All other chemicals used in this study were analytical grade and were made in China.