Male and female mice, 8–16 weeks old, weighing 20–30 g from the following strains were used: C57BL/6 (Envigo), Gad2-IRES-Cre, SST-IRES-Cre, B6 PV-Cre and VIP-IRES-Cre (The Jackson Laboratory stock #010802, #013044, #008069, and #010908, respectively). The mice were kept in a temperature-controlled facility (22–24 °C), on a 12/12 h light/dark cycle (light phase 7 A.M. to 7 P.M.) at the University of Haifa, with water and food provided ad libitum. Experimentation was approved by the University of Haifa Animal Care and Use Committee (license numbers: 437/16, 487/17, 488/17, and 631/19). Before experimentation, mice were allowed 7 d of acclimatization. Animals were handled in accordance with University of Haifa practices and standards, in compliance with the National Institutes of Health guidelines for the ethical treatment of animals.
B6 pv cre
Manufactured by Jackson ImmunoResearch
Sourced in United States, Montenegro
B6 PV-Cre is a laboratory mouse strain that carries a Cre recombinase gene under the control of the parvalbumin (PV) promoter. The Cre recombinase enzyme is expressed in a specific subset of neurons that express the parvalbumin protein.
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Lab products found in correlation
Chat ires cre, by Jackson ImmunoResearch (2 mentions)
Gad2 ires cre, by Jackson ImmunoResearch (1 mentions)
Vip ires cre, by Jackson ImmunoResearch (1 mentions)
Sst ires cre, by Jackson ImmunoResearch (1 mentions)
C57bl 6, by Jackson ImmunoResearch (1 mentions)
C57bl 6j, by Charles River Laboratories (1 mentions)
Cck ires cre, by Jackson ImmunoResearch (1 mentions)
B6 cg gt rosa, by Jackson ImmunoResearch (1 mentions)
C57bl 6j, by Jackson ImmunoResearch (1 mentions)
Trpv1 cre, by Jackson ImmunoResearch (1 mentions)
Npy2r ires cre, by Jackson ImmunoResearch (1 mentions)
C fos creert2 mouse line, by Shanghai Model Organisms (1 mentions)
Gad2 cre mice, by Jackson ImmunoResearch (1 mentions)
Pv cre mice, by Jackson ImmunoResearch (1 mentions)
6 protocols using b6 pv cre
1
Mouse Strain Characterization for Behavioral Studies
2
Optogenetic Manipulation of Neuronal Populations
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All experiments were performed using male mice. C57BL/6J (Charles River) mice were used as the background strain. The generation of the M3 receptor KO mice (CHRM3 KO) has been described53 (link). The M3 KO mice used for this study had been backcrossed for ten times onto the C57BL/6NTac background. Cre reporter allele mice (The Jackson Laboratory) were used to tag specific neuronal populations: Cholinergic neurons (Chat-IRES-Cre; Stock No. 006410), parvalbumin interneurons (B6 PVCRE; Stock No. 017320) and cholecystokinin interneurons (CCK-IRES-Cre; Stock No. 012706). Homozygous cre reporter mice were crossed with homozygous Ai32 mice (B6.Cg-Gt(ROSA)26Sortm32(CAG-COP4*H134R/EYFP)Hze/J; Stock No. 024109) to generate litters of heterozygous offspring expressing ChR2.
3
Transgenic Mouse Lines for Optogenetics
4
Genetic Labeling of Neuronal Subpopulations
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All experimental procedures were approved by the Universitat Autònoma de Barcelona Animal Experimentation Ethical Committee and followed the European Communities Council Directive 2010/63/EU and the Spanish National law (RD 53/2013). Mice were generated by breeding homozygous Ai9(RCL-tdT) mice (JAX stock #007909) (Madisen et al., 2010 (link)) with different homozygous Cre-driver lines from The Jackson Laboratory (Bar Harbor, ME, USA): ChAT-IRES-Cre (choline acetyltransferase, JAX stock #006410) (Rossi et al., 2011 (link)), B6 PVcre (parvalbumin, JAX stock #017320), Npy2r-IRES-Cre (neuropeptide Y receptor Y2, JAX stock #029285) (Barrozo et al., 2016 (link)), and TRPV1-Cre (transient receptor potential vanilloid 1, JAX stock #017769) (Cavanaugh et al., 2011 (link)). We obtained four mice lines that expressed the red fluorescent protein TdTomato under the control of a specific neuronal promoter: ChAT-Cre/Ai9, PV-Cre/Ai9, Npy2r-Cre/Ai9, and TRPV1-Cre/Ai9, respectively. The same Cre-driver lines were bred to homozygous Ribotag mice (Sanz et al., 2009 (link)). We obtained mice that expressed HA-tagged ribosomes in specific cell populations: ChAT-Cre/Ribotag, PV-Cre/Ribotag, Npy2r-Cre/Ribotag, and TRPV1-Cre/Ribotag, respectively. Mice were housed in a controlled environment (12 hr light-dark cycle, 22 ± 2°C), in open cages with water and food ad libitum.
5
Transgenic Mouse Lines for Neuroscience Research
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C57BL/6J wild-type male mice were obtained from Beijing Vital River Laboratory. GAD2-Cre mice (stock no. 028867, The Jackson Laboratory) and PV-Cre mice (stock no. 017320, The Jackson Laboratory) consisted of the GAD2-IRES-Cre and B6 PV-Cre knock-in lines, respectively. GAD67-GFP mice were a gift from N. Tamamaki (Kumamoto University) (61 (link)). The c-fos-CreERT2 mouse line (23 (link)) was obtained from Shanghai Model Organisms (stock no. NM-KI-200110) for TRAP experiments.
Mice were housed in a room maintained at a constant temperature on a 12-hour light/dark cycle (light from 08:00 to 20:00). All transgenic and knock-in mouse lines were maintained as hemizygotes. Mice had access to food and water ad libitum and were socially housed in numbers of two to five littermates randomly until surgery. Following surgery, mice were housed undisturbed. All mice were male and 3 to 5 months old for behavioral experiments. All procedures were approved by the Institutional Animal Care and Use Committee of the Fourth Military Medical University following Animal Research: Reporting of In Vivo Experiments (ARRIVE) guidelines (FMMULL-220926) and conformed to the Guide for the Care and Use of Laboratory Animals published by the National Institutes of Health. Animals were randomized to different treatments according to a group of pregenerated random numbers by Excel.
Mice were housed in a room maintained at a constant temperature on a 12-hour light/dark cycle (light from 08:00 to 20:00). All transgenic and knock-in mouse lines were maintained as hemizygotes. Mice had access to food and water ad libitum and were socially housed in numbers of two to five littermates randomly until surgery. Following surgery, mice were housed undisturbed. All mice were male and 3 to 5 months old for behavioral experiments. All procedures were approved by the Institutional Animal Care and Use Committee of the Fourth Military Medical University following Animal Research: Reporting of In Vivo Experiments (ARRIVE) guidelines (FMMULL-220926) and conformed to the Guide for the Care and Use of Laboratory Animals published by the National Institutes of Health. Animals were randomized to different treatments according to a group of pregenerated random numbers by Excel.
6
Generating Transgenic Mice Expressing ChR2
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Mice expressing the channel rhodopsin-2 (ChR2) variant ChR2 H134R fused to tdTomato in PV-INs were generated by crossing female heterozygous B6.129P2-Pvalb tm1(cre)Arbr /J (B6 PV cre , Jackson Laboratory, Bar Harbor, ME; stock number 017320) and male heterozygous B6.Cg-Gt(ROSA)26Sor tm27.1(CAG-COP4*H134R/tdTomato)Hze /J (Ai27D, Jackson Laboratory; stock number 012567) mice. After weaning, the offspring (hereafter referred to as B6 PV cre -Ai27D mice) were ear-tagged and tail snips were collected under (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission.
The copyright holder for this preprint this version posted November 11, 2020. ; https://doi.org/10.1101/2020.06. 16.155077 doi: bioRxiv preprint 10 isoflurane anesthesia. Genotyping was performed by Transnetyx (Cordova, TN). Male and female mice aged between P40 and P60 were used for all subsequent experiments (Figure 1b).
The copyright holder for this preprint this version posted November 11, 2020. ; https://doi.org/10.1101/2020.06. 16.155077 doi: bioRxiv preprint 10 isoflurane anesthesia. Genotyping was performed by Transnetyx (Cordova, TN). Male and female mice aged between P40 and P60 were used for all subsequent experiments (Figure 1b).
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