Log In Sign up
The largest database of trusted experimental protocols

Human il 6 elisa kit

Manufactured by R&D Systems
Visit Supplier
Sourced in United States

The Human IL-6 ELISA Kit is a quantitative sandwich enzyme immunoassay designed for the measurement of human interleukin-6 (IL-6) levels in cell culture supernatants, serum, and plasma samples. The kit utilizes a specific antibody coated on a microplate to capture IL-6, which is then detected using a conjugated detection antibody.

Automatically generated - may contain errors

Lab products found in correlation

Human il 8 elisa kit, by R&D Systems (3 mentions) Human il 1β elisa kit, by R&D Systems (2 mentions) Human bmp 6 elisa kit, by Thermo Fisher Scientific (2 mentions) Gdf 15, by R&D Systems (2 mentions) Human mcp 1 elisa kit, by R&D Systems (1 mentions) Mouse mcp1 elisa kit, by Abcam (1 mentions) Mouse il 6 elisa kit, by Novus Biologicals (1 mentions) Mouse tnf α elisa kit, by Novus Biologicals (1 mentions) Nheks, by Thermo Fisher Scientific (1 mentions) Nheks, by R&D Systems (1 mentions) Anti pdcd4 antibody, by Cell Signaling Technology (1 mentions) Laemmli sample buffer, by Bio-Rad (1 mentions) Beyoecl plus, by Beyotime (1 mentions) Synergy h1 hybrid plate reader, by Agilent Technologies (1 mentions) Recombinant human il 6, by Thermo Fisher Scientific (1 mentions) Ldn193189, by Selleck Chemicals (1 mentions) Lipopolysaccharides (lps), by Merck Group (1 mentions) Limulus amebocyte lysate assay, by Charles River Laboratories (1 mentions) Cobas h 232 point of care system, by Roche (1 mentions) Opteia elisa kit, by BD (1 mentions)

Spelling variants of this product

ELISAs (144 citations) Human IL-6 (49 citations) Human IL-6 Quantikine HS ELISA Kit (29 citations) Human IL-6 and IL-8 ELISA kits (6 citations) ELISA kits for human IL-6 (4 citations) Human IL-6 Quantikine enzyme-linked immunosorbent assay (ELISA) Kit (3 citations) IL-6 kits (2 citations) Human kits (2 citations) Quantikine high sensitivity human IL-6 ELISA kits (2 citations) Human IL-6 Quantikine HS ELISA Kit HS600B (2 citations)

44 protocols using human il 6 elisa kit

1

Quantifying IL-6 in SEV Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols
The amount of IL-6 in the culture medium of THP-1 cells treated with SEVs was determined using a human IL-6 ELISA kit (R&D Systems, Inc, Minneapolis, MN, USA) IL-6 protein levels were also analyzed in MM1.S- and SW480-derived SEV protein lysates. The ELISA assay was performed according to the manufacturer’s instructions.
Pucci M., Raimondo S., Urzì O., Moschetti M., Di Bella M.A., Conigliaro A., Caccamo N., La Manna M.P., Fontana S, & Alessandro R. (2021). Tumor-Derived Small Extracellular Vesicles Induce Pro-Inflammatory Cytokine Expression and PD-L1 Regulation in M0 Macrophages via IL-6/STAT3 and TLR4 Signaling Pathways. International Journal of Molecular Sciences, 22(22), 12118.
+ Open protocol
+ Expand
2

Mechanosensitive Cytokine Secretion

Check if the same lab product or an alternative is used in the 5 most similar protocols
HAECs were exposed to OSS (± 5 dyn/cm2) in the presence or absence of 5 and 10 μM G1 for 24 h. Cell culture media was collected to analyze the secretions of IL-6, IL-1β, and MCP-1. Concentrations of these proteins were assayed by ELISA using commercial kits from R&D Systems in accordance with the manufacturer’s instructions: human IL-6 ELISA kit (#D6050), human IL-1β ELISA kit (#DLB50), and human MCP-1 ELISA kit (#DCP00), human VCAM-1 ELISA kit (#DY809), human E-selectin ELISA kit (#DY724).
Chen C., Chen J., Tao X., Fu M., Cheng B, & Chen X. (2021). Activation of GPR30 with G1 inhibits oscillatory shear stress-induced adhesion of THP-1 monocytes to HAECs by increasing KLF2. Aging (Albany NY), 13(8), 11942-11953.
+ Open protocol
+ Expand
3

Cytokine Expression Analysis in Mouse and Human Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
The mouse ATDC5 cells and human C28I2 cells were inoculated in 24‐well culture plates. After treated with LPS and OI, the supernatant was centrifuged for 10 min at 1000 g and 4°C. The samples were diluted twice and analysed according to the specifications of ELISA Kits, including Mouse Il‐6 ELISA Kit (VAL604, Novus), Mouse Mcp‐1 ELISA Kit (ab100721, Abcam), Mouse Il‐10 ELISA Kit (VAL605, Novus), Mouse Tnf‐α ELISA Kit (VAL609, Novus), Human IL‐6 ELISA Kit (QK206 R&D Systems) and Human TNF‐α ELISA Kit (NBP1‐91170, Novus).
Zhang Q., Bai X., Wang R., Zhao H., Wang L., Liu J., Li M., Chen Z., Wang Z., Li L, & Wang D. (2022). 4‐octyl Itaconate inhibits lipopolysaccharide (LPS)‐induced osteoarthritis via activating Nrf2 signalling pathway. Journal of Cellular and Molecular Medicine, 26(5), 1515-1529.
+ Open protocol
+ Expand
4

Supernatant ELISA for IL-6 Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cell supernatant was centrifuged at 3000 rpm for 5 min, and then, the supernatant was collected and stored at −80℃ until analyses. IL‐6 protein level was evaluated with human IL‐6 ELISA kit (R & D Systems, USA) according to the manufacturer's instructions.
Di T., Yang Y., Fu C., Zhang Z., Qin C., Sai X., Liu J., Hu C., Zheng M., Wu Y, & Bian T. (2020). Let‐7 mediated airway remodelling in chronic obstructive pulmonary disease via the regulation of IL‐6. European Journal of Clinical Investigation, 51(4), e13425.
+ Open protocol
+ Expand
5

Polycationic Agents Modulate IL-6 in Keratinocytes

Check if the same lab product or an alternative is used in the 5 most similar protocols
Primary neonatal human epidermal keratinocytes (NHEKs) were purchased from Invitrogen. NHEKs were grown in serum free EpiLife medium supplemented with 0.06 mM CaCl2, EpiLife Defined growth supplements (EDGS) (Invitrogen) and antibiotics, and passage 4–6 cells were used for experiment. Cells at 60%–80% confluence were starved overnight without EDGS prior to treatment. NHEKs were treated with polycationic agent/peptide (10 ug/mL) or vehicle control mixed with poly(I:C) (0.3 ug/mL) for 6 hrs (RTqPCR) and 18 hrs (ELISA). IL-6 protein volume using supernatant for NHEKs after indicated stimulation were measured by human IL-6 ELISA kit (R&D systems, Minneapolis, MN) per manufacturer’s protocol. All stimulation experiments were done in triplicate. IL-6 release for each polycation-dsRNA complex was normalized relative to control stimulation and reported in Table S1.
Lee E.Y., Takahashi T., Curk T., Dobnikar J., Gallo R.L, & Wong G.C. (2017). Crystallinity of Double-Stranded RNA-Antimicrobial Peptide Complexes Modulates Toll-Like Receptor 3-Mediated Inflammation. ACS nano, 11(12), 12145-12155.
+ Open protocol
+ Expand
6

Western Blot Analysis of PDCD4 in Multiple Myeloma

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were lysed in radioimmunoprecipitation assay (RIPA) buffer in the presence of proteinase inhibitor (Biocolor BioScience & Technology Company, Shanghai, China). Cell lysates (30 μg) were denatured in Laemmli sample buffer (Bio-Rad) for 5 min at 95.1°C, separated by 10% SDS-PAGE and transferred to nitrocellulose membranes. Membranes were blocked with 5% (w/v) fat-free milk in phosphate-buffered saline (PBS) and 0.5% (v/v) Tween-20 for 1 h, and then incubated with anti-PDCD4 antibody (Cell Signaling Technology) at room temperature for 2 h. After washing, membranes were incubated with horseradish peroxidase-conjugated secondary antibody. Signals were visualized with enhanced chemiluminescence (ECL) (BeyoECL Plus, Beyotime), and analyzed using a BI-2000 system (Beyotime, Haimen, Jiangsu province, China),.
RPMI-8266 and U226 cells were treated with 75 μM and 120 μM BB, respectively.
IL6 protein levels in supernatants were determined using a human IL6 ELISA kit (R&D Systems).
Luo X., Gu J., Zhu R., Feng M., Zhu X., Li Y, & Fei J. (2014). Integrative analysis of differential miRNA and functional study of miR-21 by seed-targeting inhibition in multiple myeloma cells in response to berberine. BMC Systems Biology, 8, 82.
+ Open protocol
+ Expand
7

Quantifying IL-6 Secretion in Cultured Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
IL-6 concentration in the culture supernatants was determined using a human IL-6 ELISA kit according to manufacturer’s instructions (R & D Systems, Cat# D6050). Plates were read using a Synergy H1 Hybrid Plate Reader (Biotek Instruments, VT). The sensitivity of the assay was 0.7 pg IL-6/ml.
Ansari M.Y., Khan N.M., Ahmad N., Green J., Novak K, & Haqqi T.M. (2019). Genetic inactivation of ZCCHC6 suppresses IL-6 expression and reduces the severity of experimental osteoarthritis in mice. Arthritis & rheumatology (Hoboken, N.J.), 71(4), 583-593.
+ Open protocol
+ Expand
8

Measuring Inflammatory Markers in Acute Pancreatitis

Check if the same lab product or an alternative is used in the 5 most similar protocols
The levels of CRP in the serum of patients with AP and HCs were measured using a Human C-Reactive Protein ELISA kit (cat. no. CYT298; Merck KGaA). The levels of TNF-α and IL-6 in the serum of patients with AP were measured using a Human TNF-α Quantikine ELISA kit (cat. no. DTA00C; R&D Systems, Inc.) and Human IL-6 ELISA kit (cat. no. LS-F29154; LifeSpan BioSciences, Inc.). All processes were performed according to the manufacturers' protocols.
Yang J., Li X., Yang X., Wei H., Deng L, & Fu N. (2022). Aberrant blood cell division cycle 42 expression and its correlation with disease severity, inflammation and mortality risk in patients with acute pancreatitis. Experimental and Therapeutic Medicine, 24(1), 458.
+ Open protocol
+ Expand
9

Quantifying FDP-lysine Adducts in Plasma

Check if the same lab product or an alternative is used in the 5 most similar protocols
PC-Acro [Nε-(3-formyl-3,4-dehydropiperidino)-lysine (FDP-lysine) in protein] was determined by the method of Uchida et al. [22] (link) using ACR-LYSINE ADDUCT ELISA SYSTEM (NOF Corporation) and 0.01 ml plasma.IL-6 and CRP were measured using human IL-6 ELISA kit (R & D Systems) and N-assay LA CRP-S kit (Nittobo), respectively, according to the manufacturer's protocol.
Yoshida M., Kato N., Uemura T., Mizoi M., Nakamura M., Saiki R., Hatano K., Sato K., Kakizaki S., Nakamura A., Ishii T., Terao T., Murayama Y., Kashiwagi K, & Igarashi K. (2017). Time dependent transition of the levels of protein-conjugated acrolein (PC-Acro), IL-6 and CRP in plasma during stroke. eNeurologicalSci, 7, 18-24.
+ Open protocol
+ Expand
10

Quantifying Interleukin-6 in Culture

Check if the same lab product or an alternative is used in the 5 most similar protocols
Concentrations of IL-6 in the culture medium were determined using a human IL-6 ELISA kit (R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s protocol. Concentrations of IL-6 were determined by comparison of the optical density results with the standard curve.
Jougasaki M., Takenoshita Y., Umebashi K., Yamamoto M., Sudou K., Nakashima H., Sonoda M, & Kinjo T. (2024). Autocrine Regulation of Interleukin-6 via the Activation of STAT3 and Akt in Cardiac Myxoma Cells. International Journal of Molecular Sciences, 25(4), 2232.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!