Cells were trypsinized at passage 2. Subsequently, 1 × 105 cells per sample were washed with PBS supplemented with 2% FCS. The flow cytometry analysis utilized the following monoclonal antibodies and their respective isotype controls: PE-CD29 (Clone TS2/16, Mouse IgG, 1:50, Biolegend, San Diego, CA, USA), FITC-CD31 (Clone CO.3E1D4, IgG2a, 1:50, Biorad, Hercules, CA, USA), FITC-CD44 (Clone 25.32, IgG, 1:50, Biorad), Purified CD90 (Clone DH24A, IgM, 1:50, Monoclonal Antibody Center), FITC-PanB cells (Clone CVS36, IgG1, 1:50, Biorad). A total of 1 × 104 events were measured per sample.
Fitc panb cells
Manufactured by Bio-Rad
Sourced in United States
FITC-PanB cells are a type of cell that has been labeled with a fluorescent dye called Fluorescein isothiocyanate (FITC). These cells are commonly used in flow cytometry experiments to identify and quantify B lymphocytes, a type of white blood cell that plays a crucial role in the adaptive immune response.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using fitc panb cells
1
Phenotypic Characterization of Cells
2
Immunophenotyping of Passage 2 Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For the flow cytometry, cells were trypsinised at passage 2, and 1 × 105 cells per sample were washed with PBS +/+ supplemented with 2% FSC (Sigma Aldrich, F7524). The following monoclonal antibodies and respective isotype controls were used for the flow cytometry: PE-CD29 (Clone TS2/16, Mouse IgG, 1:50, Biolegend, San Diego, CA, USA), FITC-CD31 (Clone CO.3E1D4, IgG2a, 1:50, Biorad, Hercules, CA, USA), FITC-CD44 (Clone 25.32, IgG, 1:50, Biorad), Purified CD90 (Clone DH24A, IgM, 1:50, Monoclonal Antibody Center), FITC-PanB cells (Clone CVS36, IgG1, 1:50, Biorad). A total of 1 × 104 events were measured per sample.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!