4e1rcat

Manufactured by Selleck Chemicals

Sourced in United States

The 4E1RCat is a laboratory reagent used for research purposes. It functions as a selective antagonist for the 5-HT2C receptor. The product is intended for use in scientific research only and its specific applications should be determined by the researcher.

Automatically generated - may contain errors

Lab products found in correlation

4egi 1, by Selleck Chemicals (1 mentions) Rpmi 1640, by Thermo Fisher Scientific (1 mentions) Matrigel, by Corning (1 mentions)

2 protocols using 4e1rcat

Comprehensive NSCLC Cell Line Analysis

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NSCLC cell lines (A549, H460, H1299) were kindly provided by Dr Hua Lu at Tulane University (purchased from ATCC, Manassas, VA, USA) and cultured in RPMI‐1640 medium supplemented with 10% foetal bovine serum and 1% penicillin and streptomycin. All experiments were performed with mycoplasma‐free cells. 4EGI‐1 and 4E1RCat were purchased from SelleckChem (Houston, TX, USA). NSCLC formalin‐fixed, paraffin‐embedded (FFPE) tissue arrays were purchased from US Biomax (Derwood, MD, USA), containing normal lung tissues (n = 10), tumour and paired adjacent tissues from the same patients (n = 45), including 19 cases of LUAD (lung adenocarcinomas) and 26 cases of LUSC (lung squamous cell carcinomas). Protein arrays and analyses were performed in RayBiotech, Inc (Guangzhou, China) by using RayBio Human Protein Array G (Glass–chip‐based protein arrays containing 499 targets).

Del Valle L., Dai L., Lin H., Lin Z., Chen J., Post S.R, & Qin Z. (2021). Role of EIF4G1 network in non‐small cell lung cancers (NSCLC) cell survival and disease progression. Journal of Cellular and Molecular Medicine, 25(6), 2795-2805.

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Xenograft Tumor Growth Inhibition

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1×10⁶ AR+ parental and AR- APIPC cells were resuspended 1:1 in Matrigel (Corning): RPMI-1640 (Gibco) and subcutaneously injected into the flanks of intact or castrate NOD-scid IL2Rgamma^null mice respectively. 1×1×1 mm³ of LuCaP 173.2 tumor chunks were implanted into the flank of castrate mice. Tumor volume was calculated using the formula (L(W²))/2, where L is the length of the tumor and W the width. When tumors reached 100 mm³, animals were randomized to receive intraperitoneal injections of 15 mg/kg 4E1RCat (Selleckchem) or vehicle (5.2% PEG400 and 5.2% TWEEN80 in ddH₂O), Monday-Friday.

Liu Y., Horn J.L., Banda K., Goodman A.Z., Lim Y., Jana S., Arora S., Germanos A.A., Wen L., Hardin W.R., Yang Y.C., Coleman I.M., Tharakan R.G., Cai E.Y., Uo T., Pillai S.P., Corey E., Morrissey C., Chen Y., Carver B.S., Plymate S.R., Beronja S., Nelson P.S, & Hsieh A.C. (2019). The androgen receptor regulates a druggable translational regulon in advanced prostate cancer. Science translational medicine, 11(503), eaaw4993.

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