The tumor tissues were fixed with 4% paraformaldehyde, dehydrated in a series of graded ethanol solutions, and embedded in paraffin for slicing into 5 μm slides. Afterwards, the slides were used for subsequent hematoxylin-eosin (H&E) or immunohistochemical staining. The antibodies used for immunohistochemistry were mouse anti-Cyclin D1 (1:100 dilution, Proteintech), rabbit anti-Cleaved Caspase 3 (1:100 dilution, Proteintech), rabbit anti-E-cadherin (1:100 dilution, Proteintech), rabbit anti-Vimentin (1:100 dilution, Proteintech), rabbit anti-Nrf2 (1:100 dilution, Proteintech), rabbit anti-p62 (1:100 dilution, Proteintech), rabbit anti-LC3 (1:100 dilution, Proteintech), rabbit anti-VEGFR2 (1:100 dilution, Affinity), rabbit anti-p-STAT3 (1:100 dilution, Proteintech), rabbit anti-c-Myc (1:100 dilution, Proteintech), and rabbit anti-PD-L1 (1:100 dilution, Cell Signaling Technology). Eventually, the images were obtained under an optical microscope (Nikon).
Mouse anti cyclin d1
Manufactured by Proteintech
Sourced in China
Mouse anti-Cyclin D1 is an antibody that specifically recognizes the Cyclin D1 protein. Cyclin D1 is a cell cycle regulatory protein that plays a critical role in the G1 to S phase transition. This antibody can be used for the detection and quantification of Cyclin D1 in various applications.
Automatically generated - may contain errors
Lab products found in correlation
Rabbit anti cleaved caspase3, by Proteintech (1 mentions)
Rabbit anti e cadherin, by Proteintech (1 mentions)
Rabbit anti vimentin, by Proteintech (1 mentions)
Rabbit anti nrf2, by Proteintech (1 mentions)
Rabbit anti p62, by Proteintech (1 mentions)
Rabbit anti lc3, by Proteintech (1 mentions)
Rabbit anti vegfr2, by Affinity Biosciences (1 mentions)
Rabbit anti c myc, by Proteintech (1 mentions)
Rabbit anti pd l1, by Cell Signaling Technology (1 mentions)
Ab131215, by Abcam (1 mentions)
Ab109520, by Abcam (1 mentions)
Rabbit anti p16 ink4a, by Proteintech (1 mentions)
Rabbit anti cyclin e1, by Proteintech (1 mentions)
Mouse anti vimentin, by Proteintech (1 mentions)
E1e9v, by Cell Signaling Technology (1 mentions)
Rabbit anti e cadherin 24e10, by Cell Signaling Technology (1 mentions)
Mouse anti ubiquitin, by Cell Signaling Technology (1 mentions)
Rabbit anti cyclinb1, by Proteintech (1 mentions)
Rabbit anti vinculin, by Cell Signaling Technology (1 mentions)
Rabbit anti flag antibody, by Cell Signaling Technology (1 mentions)
6 protocols using mouse anti cyclin d1
1
Immunohistochemical Profiling of Tumor Tissue
2
Antibody Characterization for Cell Signaling
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Rabbit anti-ACAT2 (1:1000) (ab131215) and rabbit anti-P21 (1:1000) (ab109520) were purchased from Abcam. Rabbit anti-SETD7 antibody(1:1000) (24840-1-AP), rabbit anti-P16-INK4A(1:1000) (10883-1-AP), rabbit anti-P27 (1:1000)(25614-1-AP), rabbit anti-Cyclin B1 (1:1000)(55004-1-AP), rabbit anti-Cyclin E1(1:1000)(11554-1-AP), mouse anti-Cyclin D1(1:1000)(60186-1-Ig), rabbit anti-MCM2(1:1000)(10513-1-AP), rabbit anti-Cortactin (1:400)(11381-1-AP) and rabbit anti-SMA (1:1000)(14395-1-AP), mouse anti-Vimentin(1:4000)(60330-1-Ig) were purchased from Proteintech. Rabbit anti-Snail2 (1:1000) (121235) was purchased from Brickell Biotech, Inc. Antibodies against YAP1(1:1000) (A1002), TAZ (1:1000) (A23034), TEAD1(1:1000) (A5218) were purchased from AB clonal. Rabbit anti-vinculin (1:1000) (E1E9V), rabbit anti-flag antibody (1:1000) (2272S), rabbit anti-myc antibody (1:1000) (14793S), rabbit anti-p53 (7F5) (1:1000)(2527S), rabbit anti-E-Cadherin (24E10) (1:1000)(3195S), rabbit anti-N-Cadherin (D4R1H) XP®(1:1000)(13116S) and mouse anti-ubiquitin (1:1000) (#3936) were purchased from Cell Signalling Technology.
MG132 (HY-13259), Cycloheximide (CHX) (HY-12320) and Cell Counting Kit-8 (CCK-8, HY-K0301) were purchased from MedChemExpress (Shanghai, NJ, USA).
MG132 (HY-13259), Cycloheximide (CHX) (HY-12320) and Cell Counting Kit-8 (CCK-8, HY-K0301) were purchased from MedChemExpress (Shanghai, NJ, USA).
3
Investigating Macrophage Polarization Pathways
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Recombinant human IL-17A was purchased from PeproTech (USA). Rat PE-conjugated
anti-mouse CD86 and rat FITC-conjugated anti-mouse CD206 were purchased from
BioLegend (USA). Rabbit anti-GSK-3β, Arg1, β-catenin, active-β-catenin (ABC),
phospho-STAT1 (signal transducers and activators of transcription 1), and
phospho-STAT3 antibodies were products of Cell Signaling Technology (USA).
Rabbit anti-STAT6 and phosphor-STAT6 antibodies were purchased from Affinity
Biosciences (USA). Rabbit anti-iNOS antibody was purchased from Abcam (USA),
rabbit anti-p21 was a product of Santa Cruz Biotech (USA). Rabbit anti-STAT3,
SOCS3, BCL-XL, c-Myc, TCF-4, β-actin, and mouse anti-Cyclin D1 antibodies were
purchased from Proteintech (China). The Wnt signaling inhibitor XAV939 was
purchased from Santa Cruz Biotech.
anti-mouse CD86 and rat FITC-conjugated anti-mouse CD206 were purchased from
BioLegend (USA). Rabbit anti-GSK-3β, Arg1, β-catenin, active-β-catenin (ABC),
phospho-STAT1 (signal transducers and activators of transcription 1), and
phospho-STAT3 antibodies were products of Cell Signaling Technology (USA).
Rabbit anti-STAT6 and phosphor-STAT6 antibodies were purchased from Affinity
Biosciences (USA). Rabbit anti-iNOS antibody was purchased from Abcam (USA),
rabbit anti-p21 was a product of Santa Cruz Biotech (USA). Rabbit anti-STAT3,
SOCS3, BCL-XL, c-Myc, TCF-4, β-actin, and mouse anti-Cyclin D1 antibodies were
purchased from Proteintech (China). The Wnt signaling inhibitor XAV939 was
purchased from Santa Cruz Biotech.
4
Western Blot Analysis of Cell Signaling
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Cells were lysed with RIPA lysis buffer. All samples were resolved by SDS‐PAGE, transferred to nitrocellulose membranes (Whatman), and probed with the appropriate Abs. The Abs used in this study were rabbit anti‐CEACAM1 (ab108397; Abcam), rabbit anti‐N‐cadherin (22018‐1‐AP; Proteintech), mouse anti‐MMP2 (66366‐1‐IG; Proteintech), mouse anti‐E‐cadherin (60335‐1‐IG; Proteintech), mouse anti‐Cyclin D1 (60186‐1‐IG; Proteintech).
5
Alendronate-loaded Doxorubicin Nanoparticles
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Alendronate (ALN), hydroxyapatite (HA), N-hydroxysuccinimide (NHS), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), 1-ethyl-(3-dimethylaminopropyl)-carbodiimide (EDCI) and trifluoroacetic acid (TFA) were the product of Sigma-Aldrich company (St. Louis, MO). α,ω-dicarboxyl poly(ethylene glycol) (HOOC-PEG-COOH, average molecular weight was 5000 Da) was obtained from Shanghai Yare Biotech Inc.(Shanghai, China). Doxorubicin·HCl (DOX·HCl) was the product of Hisun Pharmaceutical Co. (Zhejiang province, China). 4′,6-diamidino-2-phenylindole (DAPI) was the product of Invitrogen Technologies Company (Carlsbad, USA). All other analytical grade chemicals were purchased from J&K company (Beijing, China). A549 cell was purchased from the Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Science. Primary antibodies rabbit anti-cyclin A and mouse anti-cyclin D1, and the secondary antibodies goat anti-mouse IgG and goat anti-rabbit IgG were purchased from Proteintech (USA).
Female athymic nude mice were supplied by the Experimental Animal Center, Fourth Military Medical University. All animal experimental procedures were executed in accordance with protocols that were authorized by the Animal Care and Use Committee, Fourth Military Medical University (approval date: 18/10/2014, number: 15317).
Female athymic nude mice were supplied by the Experimental Animal Center, Fourth Military Medical University. All animal experimental procedures were executed in accordance with protocols that were authorized by the Animal Care and Use Committee, Fourth Military Medical University (approval date: 18/10/2014, number: 15317).
6
Comprehensive Immunoblotting Protocol
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Mouse anti-cyclinD1 (1:1,000), and rabbit anti-p21 (1:1,000) were purchased from Protein Tech (Suzhou, China). Rabbit anti-CDK2 (1:1,000), rabbit anti-p16 (1:1,000), rabbit anti-p27 (1:1,000), and mouse anti-PCNA (1:1,000) were purchased from BOSTER (China). Rabbit anti-caspase-3 (1:1,000), rabbit anti-AKT (1:1,000), and rabbit anti-p-AKT (1:1,000) were bought from Abcam (Cambridge, UK). Rabbit anti-p53 and mouse anti-β-actin were bought from Santa Cruz Biotechnology (Heidelberg, Germany). TransIT®-2020 transfection reagent was purchased from Mirus (USA). A Cell-Light™ EdU Apollo®567 In Vitro Imaging Kit was purchased from Riobo (Guangdong, China). Cationic liposomes were produced by Sichuan University, State Key Laboratory of Biotherapy. The recombinant plasmid Biot2-shRNA was bought from Jingsai (Wuhan, China). The plasmid was prepared using the Endofree Plasmid Giga kit (Qiagen, Chatsworth, CA, USA).
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