The GC-MS analysis was performed with an Agilent 7890B gas chromatograph coupled to an Agilent 5977 mass-selective detector (Agilent Technologies, Palo Alto, CA) using the following analytical conditions: an HP-5MS fused-silica capillary column (30 m × 0.25 mm i.d.) coated with a film of 5%-phenyl-95%-methyl silicone (thickness 0.25 μm); Helium gas as carrier at a flow rate of 1.4 mL/min, sample injection volume was 1.0 μL in splitless mode and the injector port temperature was 280 °C. The oven temperature program was from 30 °C (2 min) to 300 °C (3 min) at 5 °C/min. Mass spectra were recorded in SCAN mode from 50 to 550 amu, the electron impact energy was set at 70 eV. The total run time was 59 min. The linear retention index (LRI) was calculated by injection of a mixture of n-alkanes, C7 to C30, under the same analytical conditions (Van Den Dool & Kratz 1963 (link)). Identification of the detected peaks were performed by careful comparison of the obtained MS spectra and LRI vs. data from computer library (NIST/EPA/NIH Mass spectral Library 2.0) and the literature (Adams 2007 ).
Agilent 5977
Manufactured by Agilent Technologies
Sourced in United States
The Agilent 5977 is a high-performance gas chromatograph-mass spectrometer (GC-MS) system. It is designed for a wide range of analytical applications, providing accurate and reliable data. The Agilent 5977 combines a high-efficiency gas chromatograph with a sensitive and selective mass spectrometer, enabling the separation, identification, and quantification of complex mixtures.
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Lab products found in correlation
Agilent 7890b, by Agilent Technologies (1 mentions)
Agilent 6890, by Agilent Technologies (1 mentions)
Agilent 6890b, by Agilent Technologies (1 mentions)
Hp 5ms ui capillary column, by Agilent Technologies (1 mentions)
Quant my way, by Agilent Technologies (1 mentions)
Agilent 7820, by Agilent Technologies (1 mentions)
Agilent 7890b gc system, by Agilent Technologies (1 mentions)
Hp 5ms column, by Agilent Technologies (1 mentions)
7 protocols using agilent 5977
1
GC-MS Analysis of Organic Compounds
2
Fatty Acid Determination by GC-MS
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The fatty acid determination was determined according to the method of Ge et al. [24 (link)], a volume of 100 mg of frozen sample was placed in an EP tube, and 4 mL of chloroform was added. The solution was vortexed for 30 s and mixed well. It was centrifuged at 3500 rpm for 15 min at room temperature, removed, and left standing. The lower phase was removed with an automatic pipette, transferred to another tube, and 2 mL of dichloromethane was added. The mixture was vortexed for 30 s and centrifuged for 15 min. The lower phases were combined and dried with a stream of nitrogen. After derivatization, the sample was placed in the vial to be measured and analyzed using gas chromatography coupled with mass spectrometry (GC-MS).
Chromatographic conditions: gas chromatography (Agilent 6890; Agilent Technologies, Santa Clara, CA, USA) CP-Sil 88 (100 m × 0.25 mm × 0.25 µm) column, ramp-up procedure: held at 100 °C for 5 min, ramped up to 240 °C at 4 °C/min and held for 15 min; carrier gas (He) at a flow rate of 1.0 mL/min; injection volume of 1 μL; and a splitting ratio of 10:1.
Mass spectrometry conditions: quadrupole mass spectrometry detection system (Agilent 5977; Agilent Technologies), inlet temperature 260 °C, quadrupole temperature 150 °C; scanning mode was full scan mode (SCAN), mass scan range m/z 30~550.
Chromatographic conditions: gas chromatography (Agilent 6890; Agilent Technologies, Santa Clara, CA, USA) CP-Sil 88 (100 m × 0.25 mm × 0.25 µm) column, ramp-up procedure: held at 100 °C for 5 min, ramped up to 240 °C at 4 °C/min and held for 15 min; carrier gas (He) at a flow rate of 1.0 mL/min; injection volume of 1 μL; and a splitting ratio of 10:1.
Mass spectrometry conditions: quadrupole mass spectrometry detection system (Agilent 5977; Agilent Technologies), inlet temperature 260 °C, quadrupole temperature 150 °C; scanning mode was full scan mode (SCAN), mass scan range m/z 30~550.
3
GC-FID and GC-MS Analysis of Essential Oils
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The qualitative and quantitative analysis of the EOs was carried out on HP-5MS capillary column (30 m × 0.25 mm; film thickness 0.25 μm) on Agilent 6890B gas chromatograph coupled with flame ionization detector (GC-FID) instrument coupled to Agilent 5977 mass spectrometry detector (MSD) (Agilent Technologies Inc, Santa Clara, CA, USA, USA). The samples were injected in split mode 1:20, at an inlet temperature of 220 °C. The oven temperature was set at 60 °C and increased at a rate of 3 °C/min up to 246 °C. Helium was the carrier gas (1 mL/min), while the temperature of the MSD transfer line was set to 230 °C.
Mass spectral data were collected in scan mode (m/z = 50–550), while the identification of compounds was performed using NIST (v14, National Institute of Standards and Technology, Gaithersburg, MD, USA) mass spectral database and comparison of relative retention indices (RT), as well as literature data [73 ].
Mass spectral data were collected in scan mode (m/z = 50–550), while the identification of compounds was performed using NIST (v14, National Institute of Standards and Technology, Gaithersburg, MD, USA) mass spectral database and comparison of relative retention indices (RT), as well as literature data [73 ].
4
GC-MS Analysis of Compounds
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GC–MS analyses were conducted with an Agilent 7820A gas chromatograph coupled with an Agilent 5977 mass selective detector, quadrupole type (Agilent Technologies, Santa Clara, CA). Compounds were separated on a fused-silica HP-5MS UI capillary column (30 m length, 0.25 mm inner diameter, 0.25 μm coating thickness) from Agilent, and 1 μL of sample was injected in the pulsed splitless mode. The following working conditions were employed: carrier gas, helium (1 mL/min at constant flow); injector, 250 °C; transfer line to mass selective detector, 280 °C; electron ionization (EI), 70 eV; ion source temperature, 230 °C; and mass range, 28–550 amu. Two different oven temperature programs were employed depending on the compounds to be determined, as indicated above. Oven temperature program no. 1 was from 40 °C (3 min) to 200 °C at 20 °C/min and then held at 200 °C for 1 min. Oven temperature program no. 2 was from 80 °C (1 min) to 150 °C at 10 °C/min, then to 300 °C at 20 °C/min, and finally held at 300 °C for 1 min.
5
Fatty Acid Profiling of Broiler Samples
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In total, 0.1 g of the samples was extracted in chloroform and dichloromethane mixture. Fatty acid profiles were analyzed using the Agilent gas chromatography system (Agilent 7820; Agilent Technologies, USA) with the CP-Sil 88 gas chromatographic column (100 m × 0.25 mm × 0.25 μm, Agilent, USA) selected based on the properties of different compounds, 1 μL injection volume, 10:1 split ratio, and high-purity helium as the carrier gas at a flow rate of 1.0 mL/min. Initially, the temperature of the column was maintained at 100 °C for 5.0 min. Then, it was gradually increased to 240 °C at 4 °C/min for 15 min. Subsequently, a mass spectrometry system (Agilent 5977; Agilent Technologies, USA) was used to identify and quantify the fatty acids. Data were acquired using MassHunter GC/MS Acquisition (Agilent Technologies) and analyzed using Quant-My-Way (Agilent Technologies). The detection of fatty acid composition in broilers was completed by Sanshu Biotechnology Co., Ltd.
6
Extraction and GC-MS Analysis of Organic Acids
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To extract organic acids (β-ketoadipic acid, adipic acid and levulinic acid) for detection, 500 μL of 1 M HCl, 300 μL of ethyl acetate and 100 μL of an internal standard (1,14-tetradecanedioic acid) were added to 1 ml of a cell culture sample. Subsequently, cells were disrupted by bead beating (FastPrep-24TM 5G and acid-washed beads (≤ 106 μm) running at 6.5 m/s and 1 min interval 4 times) and centrifuged for 10 min at 20,000 x g at 4°C to separate the organic phase. The ethyl acetate extracts were incubated with a derivatization agent [BSTFA with 1% trimethylchlorosilane (TCMS)] overnight prior to analysis by gas-liquid chromatography (GC) using an Agilent 7890B GC system equipped with an HP-5MS column (Agilent) coupled to a mass spectrometer (Agilent 5977).
7
GC-MS Analysis of Complex Samples
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The equipment employed for GC-MS analyses was an Agilent 7820A gas chromatograph coupled with an Agilent 5977 mass selective detector, quadrupole type (Agilent Technologies, Santa Clara, CA). The pulsed splitless mode was used to inject one microliter of sample, which was fractionated on a fused-silica HP-5MS UI capillary column (30 m length, 0.25 mm inner diameter, 0.25 m coating thickness). The conditions employed were: carrier gas, helium (1 mL/min at constant flow); injector, 250 ºC; transfer line to mass selective detector, 280 ºC; electron ionization (EI), 70 eV; ion source temperature, 230 ºC; and mass range, 28-550 amu. The oven was programmed from 40 ºC (3 min) to 200 ºC at 20 ºC/min, and then held at 200 ºC for 1 min.
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