Mycoplasma detection assay

Manufactured by Lonza

The Mycoplasma detection assay is a laboratory equipment product designed to detect the presence of Mycoplasma species in samples. It is a tool used in research and quality control applications.

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Lab products found in correlation

Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) Rpmi 1640 medium, by Nacalai Tesque (1 mentions) Fetal bovine serum (fbs), by Biowest (1 mentions) Hek293t, by American Type Culture Collection (1 mentions)

Spelling variants of this product

MycoAlert Mycoplasma Detection Assay (32 citations) MycoAlert mycoplasma detection assay kit (3 citations) MycoAlert® Plus Mycoplasma Detection Assay (2 citations)

2 protocols using mycoplasma detection assay

Cell Line Verification and Culture

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HGC-27 (RRID:CVCL_1279), LMSU (RRID:CVCL_4849), and HEK293T (RRID:CVCL_0063) were purchased from CellBank Australia, JCRB cell bank, and ATCC, respectively. They were cultured in RPMI1640 Medium (Nacalai tesque), supplemented with 10% FBS (Biowest) and 1% Penicillin-Streptomycin (Gibco by Life Technologies). Cell lines were authenticated by DNA profiling (Promega). All cell lines were tested regularly to be Mycoplasma-free by Mycoplasma detection assay (Lonza).

Suda K., Okabe A., Matsuo J., Chuang L.S., Li Y., Jangphattananont N., Mon N.N., Myint K.N., Yamamura A., So J.B., Voon D.C., Yang H., Yeoh K.G., Kaneda A, & Ito Y. (2024). Aberrant Upregulation of RUNX3 Activates Developmental Genes to Drive Metastasis in Gastric Cancer. Cancer Research Communications, 4(2), 279-292.

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Establishment and Characterization of ATC Cell Lines

Cited 1 time

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The 8505C BRAF-mutated ATC cell line was obtained from DSMZ; HeLa
cervical cancer cells and HEK 293T human embryonic kidney cells were obtained
from ATCC. The JV cell line was derived previously by our group from fresh
tissue culture of a surgical ATC tumor specimen and was used to establish
patient-derived xenograft model (21 (link)). FRO
and KHM-5M cell lines were generously provided by Dr. Juillard’s
laboratory at UCLA and Dr. Fagin’s laboratory at MSKCC, respectively. All
epithelial cell lines were cultured in RPMI media supplemented with 10% fetal
bovine serum (FBS) at 37°C with 5% CO₂. Cell lines were stably
transduced with a lentiviral vector encoding fLuc-F2A-GFP lentivirus (Biosettia)
and were used in effector to target (E:T) experiments and in
vivo tracking within xenograft models. Mycoplasma detection assay
(Lonza) was routinely conducted to rule out contaminated cell lines.

Gray K.D., McCloskey J.E., Vedvyas Y., Kalloo O., Eshaky S.E., Yang Y., Shevlin E., Zaman M., Ullmann T., Liang H., Stefanova D., Christos P.J., Scognamiglio T., Tassler A., Zarnegar R., Fahey TJ I.I.I., Jin M.M, & Min I.M. (2020). PD1 blockade enhances ICAM1-directed CAR T therapeutic efficacy in advanced thyroid cancer. Clinical cancer research : an official journal of the American Association for Cancer Research, 26(22), 6003-6016.

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