Quercitrin and gallic acid (3,4,5-trihydroxy benzoic acid, 99%) and Folin–Ciocalteu phenol reagent, Na2CO3, formic acid (HPLC grade), 1-diphenyl-2-picryl-hydrazyl (DPPH), and Streptomycin was procured from Sigma Aldrich (Steinheim, Germany). Ethanol (reagent grade) was purchased from Across Organics (Leicestershire, England). Methanol (HPLC grade) and Acetonitrile (HPLC grade) were obtained from Daejung Company, Ltd. (Busan, South-Korea).
1 diphenyl 2 picryl hydrazyl dpph
Manufactured by Merck Group
Sourced in India
1-diphenyl-2-picryl-hydrazyl (DPPH) is a stable free radical used as a reagent in biochemical and analytical applications. Its core function is to measure the antioxidant activity of various compounds.
Automatically generated - may contain errors
Lab products found in correlation
Ascorbic acid, by Merck Group (3 mentions)
Folin ciocalteu phenol reagent, by Merck Group (1 mentions)
Hplc grade formic acid, by Merck Group (1 mentions)
Na2co3, by Merck Group (1 mentions)
Streptomycin, by Merck Group (1 mentions)
2 thiobarbituric acid tba, by Merck Group (1 mentions)
L 1 agar, by Merck Group (1 mentions)
L 1 peptone, by Merck Group (1 mentions)
Gallic acid, by Merck Group (1 mentions)
Folin ciocalteu reagent, by Merck Group (1 mentions)
Aquadest, by Merck Group (1 mentions)
2 2 azino bis, by Merck Group (1 mentions)
Ethanol, by Merck Group (1 mentions)
N hexane, by Merck Group (1 mentions)
6 protocols using 1 diphenyl 2 picryl hydrazyl dpph
1
Antioxidant Assay Protocol
2
Antioxidant and Anti-inflammatory Assays
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All the chemicals were of analytical grade and milliQ water (Milli-Q® Integral 3 Water Purification System) was used wherever required. 1, 1- diphenyl-2-picryl hydrazyl (DPPH) (Sigma–Aldrich), 2-Thiobarbituric acid (TBA) (Sigma–Aldrich), carrageenan (Himedia, Irish Moss; RM 1576), ascorbic acid (vitamin C) and α-tocopherol (vitamin E) (Sigma–Aldrich) as antioxidant standards, and aspirin (Disprin, Reckitt Benckiser India) as a standard anti-inflammatory drug were used in the experiments. Phosphate buffer saline (PBS) was prepared in milliQ water.
3
Microbiological and Antioxidant Evaluation of Polyamide Fabrics
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Nutrient broth (NB) consisting of 3 g L-1 meat extract, 5 g L-1 peptone and salts was used as a medium for bacterial cultures. Nutrient agar petri-dishes were prepared with composition 3 g L-1 meat extract, 5 g L-1 peptone, and 15 g L-1 agar. Sabouraud medium was used for maintenance of Candida spp. and it contained 40 g L-1 glucose, 10 g L-1 peptone, 20 g L-1 agar, pH 5.6.
1,1-Diphenyl-2-picryl-hydrazyl (DPPH) was purchased from Sigma (St. Louis, MO, United States). Methanol used was of HPLC grade. Polyamide knitted fabric with density 23 columns cm-1 and 15 rows cm-1, weight 12 g m-2, 154.7 Denier and average thickness 0.309 mm was supplied by Colora S.A. (Thessaloniki, Greece). The PA fabric was washed with detergent Felosan NFG before use for the removal of impurities.
1,1-Diphenyl-2-picryl-hydrazyl (DPPH) was purchased from Sigma (St. Louis, MO, United States). Methanol used was of HPLC grade. Polyamide knitted fabric with density 23 columns cm-1 and 15 rows cm-1, weight 12 g m-2, 154.7 Denier and average thickness 0.309 mm was supplied by Colora S.A. (Thessaloniki, Greece). The PA fabric was washed with detergent Felosan NFG before use for the removal of impurities.
4
Antioxidant Capacity Determination
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1, 1-Diphenyl-2-picrylhydrazyl (DPPH), gallic acid, Folin-Ciocalteu reagent, ascorbic acid, and SRB were purchased from Sigma-Aldrich. All other chemicals were of analytical grade and obtained from Himedia Company.
5
Antioxidant Evaluation of Phytochemicals
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Methanol, ethanol, acetone, gallic acid, HCl, Lascorbic acid, petroleum ether (PET), folinciocalteau reagent, quercetin, sodium hydroxide, boric acids were purchased from SRL Pvt Ltd (Mumbai, Maharashtra, India). 1-Diphenyl-2Picrylhydrazyl (DPPH) purchased from Sigma-Aldrich, India.
All chemicals used in the experiment were analytical grade.
All chemicals used in the experiment were analytical grade.
6
Antioxidant Evaluation of Chromolaena odorata Leaves
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The tools used are maceration tools, test kits for chemical content, glass tools, UV-Vis spectrophotometer (Agilent ® ), ELISA reader (Biotek ® ), and analytical scales. The materials used were the leaves of Botto-Botto (Chromolaena odorata L.), ethanol (Merck), N-hexane (Merck), Aquadest (Merck), ascorbic acid (Merck), 1-diphenyl-2-picrylhydrazyl (DPPH) (Sigma-Aldrich), 2, 2'-azinobis (3-ethylbenzothiazoline-6-sulphonic acid diammonium salt) (ABTS) (Sigma-Aldrich).
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