LGR5+ HFSCs were purified as previously described from anagen-synchronized HFs of Ring1a/b+/+ mice and were extracted following the protocol previously described. A total of 2.5 million cells were used for ChIP-seq as previously described (9 (link)). Sonicated chromatin was incubated with 10 μg of rabbit anti-RING1B antibody (homemade), anti-H2AK119Ub1 (8240, Cell Signaling Technology), anti-RYBP (AB3637, Millipore), and anti-CBX8 (39 (link)) overnight, and the immunocomplexes recovered using protein A–conjugated magnetic beads (Dynabeads, Life Technologies). Purified chromatin was decross-linked overnight in 0.1 M NaHCO3 and 1% SDS. Decross-linked DNA was purified. ISC-specific H3K4me3 ChIP-seq profile was obtained as previously described (9 (link)) using 5 μg of anti-H3K4Me3 antibody (catalog no. 39159, Active Motif).
Anti h2ak119ub1
Manufactured by Cell Signaling Technology
Anti-H2AK119Ub1 is a primary antibody that recognizes histone H2A monoubiquitinated at lysine 119. This modification is associated with transcriptional repression and plays a role in X-chromosome inactivation and polycomb-mediated gene silencing.
Automatically generated - may contain errors
Lab products found in correlation
Dynabead, by Thermo Fisher Scientific (1 mentions)
Anti rybp, by Merck Group (1 mentions)
Anti h3k4me3 antibody, by Active Motif (1 mentions)
Anti flag, by Merck Group (1 mentions)
Ab9049, by Abcam (1 mentions)
Anti h3k27me3, by Cell Signaling Technology (1 mentions)
Anti suz12 d39f6, by Cell Signaling Technology (1 mentions)
Sc 32921, by Santa Cruz Biotechnology (1 mentions)
Anti jarid2, by Cell Signaling Technology (1 mentions)
Anti aebp2, by Cell Signaling Technology (1 mentions)
Ab201510, by Abcam (1 mentions)
2 protocols using anti h2ak119ub1
1
ChIP-seq of LGR5+ Hair Follicle Stem Cells
2
Comprehensive Epigenetic Regulation Analysis
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Western blot and ChIP analyses were performed with: anti-BAP1 (D7W70; Cell Signaling), anti-Suz12 (D39F6; Cell Signaling Technology), anti-FLAG (F3165; Sigma-Aldrich), anti-H3K27me3 (9733; Cell Signaling Technology), anti-H2AK119ub1 (8240; Cell Signaling Technology), anti-H3 (ab1791; Abcam), anti-H2A (07-146; Sigma-Aldrich), anti-RING1B (homemade (Chiacchiera et al., 2016 (link))), anti-PCGF1, anti-PCGF2, and anti-PCGF6 (Pasini’s lab, homemade (Scelfo et al., 2019 (link))), anti-JARID2 (13594, Cell Signaling Technology), anti-AEBP2 (14129; Cell signaling Technology), anti-HA (C29F4, Cell Signaling Technology), anti-ASXL2 (A302-037A, Bethyl), anti-ASXL1 (D1B6V, Cell Signaling Technology) anti-KDM1B/LSD2 (E1R60, Cell Signaling Technology), anti-OGT (SC-32921, Santa-Cruz), anti-HCFC1 (A301-400A, Bethyl), anti-FOXK2 (A301-730A, Bethyl), anti-MBD6 (SAB1305225-40TST, Sigma-Aldrich), anti-H3K27ac (AB4729, Abcam) anti-PCGF3/5 (AB201510, Abcam), anti-UTX (Helin lab, homemade (Agger et al., 2007 (link))), anti-MTF2 (Proteintech 16208-1-AP), anti-CBX7 (abcam, ab21873), anti-RYBP/DEDAF (Sigma-Aldrich, PRS2227), anti-H3K36me2 (abcam, ab9049), anti-RING1B-pS168 (abcam, ab234421).
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