Hnrnpg rbmx nm 002139 human tagged orf clone

Leukemia cells were cultured in RPMI media (Cellgro) supplemented with 10% FBS, glutamine, and 1% penicillin/streptomycin at 37°C and 5% CO₂. MOLM13, KCL-22, Kasumi-1, K562, and THP-1 cells were transduced with lentivirus expressing either control scramble shRNA (Addgene #1864) or shRNAs targeting RBMX and RBMXL1 (shRNA1 and shRNA2). 1- or 2-days after transduction, depending on the cell line, cells were treated with 3 μg/ml puromycin for 2 days after puromycin selection. For proliferation assays, cells were then plated at 250,000 cells/ml and counted every 24 hours at the indicated timepoints using a hemocytometer. MOLM13 cells were transduced with lentivirus expressing either control scramble shRNA (Addgene #1864) or shRNAs targeting CBX5 (shRNA368 and shRNA704). 2-days after transduction, cells were treated with 3 μg/ml puromycin for 2 days and then plated at 250,000 cells/ml. For overexpression experiments, KCL-22 cells were transduced with MSCV-IRES-GFP (Addgene #20672) retrovirus expressing either empty vector as control or Flag-RBMX. RBMX was cloned into MSCV-IRES-GFP vector from Origene hnRNPG (RBMX) (NM_002139) Human Tagged ORF Clone.