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Irdye 680lt donkey anti goat igg

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IRDye® 680LT Donkey anti-Goat IgG is a fluorescently-labeled secondary antibody produced in donkeys and targeted against goat immunoglobulin G (IgG). It is designed for use in fluorescence-based detection and quantification applications.

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Lab products found in correlation

Odyssey infrared imager, by LI COR (4 mentions) Irdye 800cw donkey anti mouse igg, by LI COR (4 mentions) Anti c myc monoclonal antibody, by Roche (3 mentions) Arp7 polyclonal antibody, by Santa Cruz Biotechnology (3 mentions) Irdye 680lt streptavidin, by LI COR (1 mentions) Irdye 680lt donkey anti rabbit igg, by LI COR (1 mentions) Irdye 800cw donkey anti rabbit igg, by LI COR (1 mentions)

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IRDye 680LT (112 citations) Donkey anti-goat IgG (6 citations)

5 protocols using irdye 680lt donkey anti goat igg

1

Quantitative Angiogenesis Imaging Assay

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All recombinant growth factors and their respective receptors were obtained commercially from R&D Systems. Antibodies and other reagents were obtained commercially: anti-human VEGF (AF-293-NA), anti-human VEGF-C (AF752), anti-human PlGF (AF-264-PB), anti-human PDGF-BB (AF-220-NA), anti-human FGF-basic (AF-233-NA), anti-mouse/rat NRP-1 (AF566), and anti-mouse/rat NRP-2 (AF567) were from R&D Systems; anti-fd Bacteriophage-Biotin Conjugate (B2661) was from Sigma-Aldrich; secondary antibodies IRDye 680LT Donkey anti-goat IgG and IRDye 680LT Streptavidin were from LI-COR. The gas admixture (75% oxygen and 25% nitrogen) used for the OIR mouse model was obtained from Air Products.
Michaloski J.S., Redondo A.R., Magalhães L.S., Cambui C.C, & Giordano R.J. (2016). Discovery of pan-VEGF inhibitory peptides directed to the extracellular ligand-binding domains of the VEGF receptors. Science Advances, 2(10), e1600611.
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2

Whole Cell Extract Preparation and Western Blot Analysis

Cited 1 time
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Whole cell extracts were prepared using the TCA extraction method, essentially as described35 (link). Samples were analyzed by standard SDS-PAGE and Western blotting. Anti-c-myc monoclonal antibody (Roche; 11667149001) was diluted 1:1,000 to detect Rfc1-AID; Arp7 polyclonal antibody (Santa Cruz, SC-8961) was used at 1:5,000 dilution as a loading control. Secondary antibodies, used at 1:5,000 dilution, were IRDye® 800CW Donkey anti-Mouse IgG (LI-COR, 925–32212) and IRDye® 680LT Donkey anti-Goat IgG (LI-COR, 925–68024). Membranes were imaged with an Odyssey Infrared Imager (LI-COR). Quantification of protein bands was performed using Image Studio Lite 5.0.21 software.
Kulkarni D.S., Owens S.N., Honda M., Ito M., Yang Y., Corrigan M.W., Chen L., Quan A.L, & Hunter N. (2020). PCNA activates the MutLγ endonuclease to promote meiotic crossing over. Nature, 586(7830), 623-627.
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3

Protein Extraction and Western Blot Analysis

Cited 1 time
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TCA extractions were performed as described (Johnson and Blobel, 1999 (link)). Samples were analyzed by standard SDS-PAGE and Western analysis. Primary antibodies targeting the following tags or proteins were used: mouse anti-HA (12CA5) (1:2,000, Millipore Sigma), rabbit anti-Smt3 (1:500, a gift from Dr. Steve Brill), Arp7 (1:10,000, Santa Cruz, SC-8960), anti-Msh4 and anti-Msh5 (1:500, a gift from Dr. Akira Shinohara). Secondary antibodies (1:10,000) were IRDye® 800CW Donkey anti-Mouse IgG (LI-COR, 925–32212), IRDye® 680LT Donkey anti-Goat IgG (LI-COR, 925–68024), IRDye® 680LT Donkey anti-Rabbit IgG (LI-COR, 925–68023) and IRDye® 800CW Donkey anti-Rabbit IgG (LI-COR, 925–32213). Western blots were imaged on an Odyssey Infrared Imager (LI-COR) and quantification of protein bands was performed using Image Studio Lite Ver 4.0 software.
He W., Verhees G.F., Bhagwat N., Yang Y., Kulkarni D.S., Lombardo Z., Lahiri S., Roy P., Zhuo J., Dang B., Snyder A., Shastry S., Moezpoor M., Alocozy L., Lee K.G., Painter D., Mukerji I, & Hunter N. (2021). SUMO Fosters Assembly and Functionality of the MutSγ Complex to Facilitate Meiotic Crossing Over. Developmental cell, 56(14), 2073-2088.e3.
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4

Whole Cell Extract Preparation and Western Blot Analysis

Cited 1 time
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Whole cell extracts were prepared using the TCA extraction method, essentially as described35 (link). Samples were analyzed by standard SDS-PAGE and Western blotting. Anti-c-myc monoclonal antibody (Roche; 11667149001) was diluted 1:1,000 to detect Rfc1-AID; Arp7 polyclonal antibody (Santa Cruz, SC-8961) was used at 1:5,000 dilution as a loading control. Secondary antibodies, used at 1:5,000 dilution, were IRDye® 800CW Donkey anti-Mouse IgG (LI-COR, 925–32212) and IRDye® 680LT Donkey anti-Goat IgG (LI-COR, 925–68024). Membranes were imaged with an Odyssey Infrared Imager (LI-COR). Quantification of protein bands was performed using Image Studio Lite 5.0.21 software.
Kulkarni D.S., Owens S.N., Honda M., Ito M., Yang Y., Corrigan M.W., Chen L., Quan A.L, & Hunter N. (2020). PCNA activates the MutLγ endonuclease to promote meiotic crossing over. Nature, 586(7830), 623-627.
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5

Western Blot Analysis of Rfc1-AID and Arp7

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Whole cell extracts were prepared using the TCA extraction method, essentially as described 43 . Samples were analyzed by standard SDS-PAGE and Western blotting. Anti-c-myc monoclonal antibody (Roche; 11667149001) was diluted 1:1,000 to detect Rfc1-AID; Arp7 polyclonal antibody (Santa Cruz, SC-8961) was used at 1:5,000 dilution as a loading control.
Secondary antibodies, used at 1:5,000 dilution, were IRDye® 800CW Donkey anti-Mouse IgG (LI-COR, 925-32212) and IRDye® 680LT Donkey anti-Goat IgG (LI-COR, 925-68024).
Membranes were imaged with an Odyssey Infrared Imager (LI-COR). Quantification of protein bands was performed using Image Studio Lite 5.0.21 software.
Kulkarni D.S., Owens S., Honda M., Ito M., Yang Y., Corrigan M.W., Chen L., Quan A.L., & Hunter N. (2020). PCNA activates the MutLγ endonuclease to promote meiotic crossing over.
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