Ifit2

Samples were lysed in RIPA lysis buffer system (Santa Cruz) according to manufacturer’s instructions. 50 µg of protein in cell lysates were separated in 4–20% SDS polyacrylamide gels and transferred to nitrocellulose membranes. Membranes were incubated with the following primary antibodies against IFIT1 (Cell Signaling), IFIT2 (Abcam), IFIT3 (Abcam), cleaved PARP (Cell signaling), BCL2 (Cell signaling) and β-actin (Cell Signaling). Anti-rabbit and anti-mouse IgG antibodies tagged with horseradish peroxidase (Cell signaling) were used. Uncropped scans of the blots are available in Supplementary Figs. 7 and 8.

Protein lysates were collected and analyzed by western blot as previously described^{24 (link)}. In brief, primary antibodies against GAPDH (Cell Signaling, 5174), Lamin A/C (Cell Signaling, 4777), p-Akt1 Ser 473 (Cell Signaling, 4060), total Akt1 (Cell Signaling, 4691), IFIT2 (Abcam - ab113112), Viperin (Cell Signaling -13996 S), Actinin 2 (Thermo Fisher Scientific - PA5-27863), Alpha-Pix (Cell Signaling -4753 S), RVFV Nucleoprotein (BEI Resources, NR-43188), or HRP-conjugated actin (Abcam, ab49900) were diluted in 3% milk solution per the manufacturer’s recommended dilutions followed by the addition of the appropriate secondary antibody. Cropped, representative images are shown in main figures, but uncropped originals can be found in the Supplemental Figure 8.