Mice were fasted for 12 h. Blood samples were collected from mice before an i.v. injection by tail vein of 2 mg g−1 body weight of glucose (20% glucose; Glucosteril, Fresenius Kabi, Bad Homburg, Germany). Additional blood samples were collected 2, 5, 15, 30 and 60 min after the injection. Serum insulin levels were determined by enzyme-linked immunosorbent assay according to the manufacturer's instructions (mouse/rat Insulin ELISA, Crystal Chem Inc., Downers Grove, IL, USA).
Mouse rat insulin elisa
Manufactured by Crystal Chem
Sourced in United States
The Mouse/rat Insulin ELISA is a quantitative sandwich enzyme-linked immunosorbent assay designed for the measurement of insulin in mouse and rat samples. The assay employs antibodies specific for insulin and utilizes a colorimetric detection system. The core function of this product is to provide a reliable and sensitive method for the determination of insulin levels.
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3 protocols using mouse rat insulin elisa
1
Glucose Tolerance Test in Mice
2
Metabolic Biomarkers in Mice
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Blood glucose values were determined from whole venous blood samples using an automated
glucose monitor (FreeStyle mini, Abbott GmbH, Ludwigshafen, Germany). Insulin, estradiol, progesteron and adiponectin serum concentrations were measured by ELISA using mouse standards according to the manufacturer’s guidelines (Mouse/Rat Insulin ELISA; CrystalChem. Inc, Downers Grove, IL), (Mouse/Rat Estradiol ELISA; Calbiotech Inc, Spring Valley, CA), (Mouse/Rat Progesterone ELISA; BioVendor, Karasek, Czech Republic) and (Mouse Adiponectin ELISA; AdipoGen Inc, Incheon, Korea). Serum concentrations of triglycerides and total cholesterol were analyzed by an automatic chemical analyzer in our Institute of Laboratory Medicine and Clinical Chemistry.
glucose monitor (FreeStyle mini, Abbott GmbH, Ludwigshafen, Germany). Insulin, estradiol, progesteron and adiponectin serum concentrations were measured by ELISA using mouse standards according to the manufacturer’s guidelines (Mouse/Rat Insulin ELISA; CrystalChem. Inc, Downers Grove, IL), (Mouse/Rat Estradiol ELISA; Calbiotech Inc, Spring Valley, CA), (Mouse/Rat Progesterone ELISA; BioVendor, Karasek, Czech Republic) and (Mouse Adiponectin ELISA; AdipoGen Inc, Incheon, Korea). Serum concentrations of triglycerides and total cholesterol were analyzed by an automatic chemical analyzer in our Institute of Laboratory Medicine and Clinical Chemistry.
3
Metabolic Biomarker Measurement Protocol
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Plasma insulin levels were measured using mouse/rat insulin ELISA (Crystal Chem., Downers Grove, USA). Plasma FFAs were measured by NEFA-HR(2) Assay (WAKO Chemicals, Neuss, Germany). Liver and muscle DAGs were measured by liquid chromatography-mass spectrometry/mass spectrometry in the lab of Gerald I. Shulman (Yale University, USA) as described previously [77 (link)]. Hepatic TAG content was determined using a triglyceride assay (DiaSys, Holzheim, Germany) following the manufacturer's instructions. Inflammation markers in plasma samples were analyzed by electrochemiluminescence assays (Meso Scale Discovery, Rockville, USA).
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