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Nis elements c software

Manufactured by Nikon
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Sourced in Japan, United States

NIS-Elements C is a software suite designed for image acquisition, analysis, and processing. It provides a comprehensive platform for managing and manipulating digital microscopy data. The software supports a wide range of microscopy techniques and enables users to capture, process, and analyze high-quality images.

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Lab products found in correlation

A1r laser scanning confocal microscope, by Nikon (4 mentions) Inubg2h tizb, by Tokai Hit (2 mentions) Rhodamine conjugated phalloidin, by Thermo Fisher Scientific (2 mentions) A1si spectral detector confocal system, by Nikon (2 mentions) A1si confocal microscope, by Nikon (2 mentions) A1r hd25, by Nikon (1 mentions) Image pro 10, by Media Cybernetics (1 mentions) Neon transfection system, by Thermo Fisher Scientific (1 mentions) Calcofluor white m2r, by Merck Group (1 mentions) A1 confocal laser microscope series, by Nikon (1 mentions) Prolong gold antifade mounting medium, by Thermo Fisher Scientific (1 mentions) Hoechst 33258, by Thermo Fisher Scientific (1 mentions) A1 laser scanning confocal microscope, by Nikon (1 mentions) Plan apo λ 20 objective, by Nikon (1 mentions) Dmem f12 1 1, by Thermo Fisher Scientific (1 mentions) Imarisviewer software, by Oxford Instruments (1 mentions) Prolong diamond, by Thermo Fisher Scientific (1 mentions) Af501, by R&D Systems (1 mentions) A1 confocal microscope, by Nikon (1 mentions) A1r confocal system, by Nikon (1 mentions)

29 protocols using nis elements c software

1

Intracellular Organelle Imaging in HPAECs

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PH-PLCD1-GFP, PH-PLCD1-iRFP, and TfR-GFP plasmids were purchased from Addgene (plasmids 51407, 66841, and 45060, respectively) and were transfected into cultured HPAECs using the Neon Transfection System (Thermo Fisher) 2 d before imaging under optimized conditions (1,270 V, 20-ms pulse length, with two pulses). All of the images were captured using a confocal laser-scanning microscope (A1R HD25; Nikon) equipped with ×60 (ApoTIRF) and ×100 (SR ApoTIRF) objectives at the following excitation/emission wavelengths: EGFP-D4, PH-PLCD1-GFP, and TfR-GFP (488, 525/50 nm); mCherry-D4 (561, 595/50 nm); and PH-PLCD1-iRFP (640, 700/75 nm) using NIS-Elements C software (Nikon). The obtained images were merged using Adobe Photoshop software. Signals of D4-based sensors were quantitatively analyzed by Image Pro-10 software (Media Cybernetics).
Yamamoto K., Nogimori Y., Imamura H, & Ando J. (2020). Shear stress activates mitochondrial oxidative phosphorylation by reducing plasma membrane cholesterol in vascular endothelial cells. Proceedings of the National Academy of Sciences of the United States of America, 117(52), 33660-33667.
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2

3D Imaging of Alexa546-agLDL Uptake

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BMMs incubated with Alexa546–agLDL for 1 h were fixed with 3% PFA for 20 min and washed with PBS. Cells were next stained with Alexa488–phalloidin (0.02 U/ml) in 0.5% saponin (w/v) in PBS for 1 h, washed 3 times with PBS and then imaged. Imaging was performed on a Nikon A1R Laser Scanning Confocal Microscope (Nikon Corporation, Melville, NY) using a 60× Oil 1.4 NA objective. Z-stacks were obtained using a step size of 1 μm (total z-size of 29 μm). A 3D reconstruction from these z-stacks, and a movie rotating the 3D reconstruction along the x axis was then created using NIS-Elements C software (Nikon Corporation).
Singh R.K., Barbosa-Lorenzi V.C., Lund F.W., Grosheva I., Maxfield F.R, & Haka A.S. (2016). Degradation of aggregated LDL occurs in complex extracellular sub-compartments of the lysosomal synapse. Journal of Cell Science, 129(5), 1072-1082.
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3

Visualizing Isoeugenol's Effect on Aspergillus Biofilm

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The biofilm of A. fumigatus was cultured in a 12-well polystyrene plate at calculated IC50 of isoeugenol to analyse its effect (Manavathu et al. 2014 (link)). To check biofilm eradication property of isoeugenol, biofilm was fully grown on 12-well polystyrene plate for 48 h and then, treated with IC50 of isoeugenol for another 24 h (Hoda et al. 2019 (link); Sav et al. 2018 (link)). To visualise the effect of isoeugenol, scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM) were performed and samples were processed as described by González-Ramírez et al (2016 (link)), with minor modifications. The A. fumigatus biofilm topology of isoeugenol treated and control (untreated) were viewed under SEM (Zeiss SEM, MA EVO -18 Special Edition). For CLSM, samples were stained with calcofluor white M2R (Sigma, St. Louis, MO, USA) and viewed under Nikon Instruments A1 Confocal Laser Microscope Series with NIS elements C software, Japan.
Gupta L., Sen P., Bhattacharya A.K, & Vijayaraghavan P. (2022). Isoeugenol affects expression pattern of conidial hydrophobin gene RodA and transcriptional regulators MedA and SomA responsible for adherence and biofilm formation in Aspergillus fumigatus. Archives of Microbiology, 204(4), 214.
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4

Visualizing Osteoclast Morc3 Protein

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Osteoclasts cultured on cover slips were fixed with 4% paraformaldehyde and permeabilised using in 0.1% Triton X-100. The osteoclasts were then incubated with primary anti-mouse Morc3 antibody (MBL International. Japan). F-actin was stained with rhodamine-conjugated phalloidin (Molecular Probes, USA). The nuclei were stained with Hoechst 33258 (Molecular Probes, USA). The samples were then incubated with a FITC-conjugated secondary anti-mouse IgG antibody (Sigma-Aldrich, USA) and mounted onto glass slides with Prolong Gold antifade mounting medium (Invitrogen). Morc3 protein and F-actin stain were visualized and imaged using the Nikon Ti-E inverted motorized microscope with Nikon A1Si spectral detector confocal system (Nikon) running on the NIS-Elements C software (Nikon).
Jadhav G., Teguh D., Kenny J., Tickner J, & Xu J. (2016). Morc3 mutant mice exhibit reduced cortical area and thickness, accompanied by altered haematopoietic stem cells niche and bone cell differentiation. Scientific Reports, 6, 25964.
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5

Measuring Mitochondrial Membrane Potential

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Activated T cells were plated onto polyethyleneimine-coated glass coverslips and incubated for 30 min at 37°C with 5 nM potentiometric probe tetramethylrhodamine methyl ester (TMRM; Invitrogen), a lipophilic cationic dye that accumulates in the mitochondrial matrix in the amount proportional to the magnitude of mitochondrial inner membrane potential (ΔΨm) [44 (link)], as previously described [45 (link)–47 (link)]. Coverslips with adherent cells were mounted into a recording chamber and placed on the stage of a Nikon A1 laser scanning confocal microscope (Nikon Instruments Inc.). The recording chamber was continuously perfused with solutions containing 5 nM TMRM. During time-lapse recording, TMRM was excited with a 543-nm laser line, and emission was detected using a 565–605 nm band-path filter. At the end of each experiment, 10 μM carbonyl cyanide phenyl hydrazone (FCCP) was added to the bath solution to dissipate ΔΨm. Experiments were performed at room temperature in air. Image analysis was performed using Nikon NIS-Elements C software. In each experiment, the TMRM fluorescence intensity values were normalized such that the TMRM fluorescence intensity level recorded 30 min after FCCP application was set to 0, whereas the TMRM fluorescence intensity level before the first solution exchange was set to 1.
Yang L., Dedkova E.N., Allen P.D., Jafri M.S, & Fomina A.F. (2020). T lymphocytes from malignant hyperthermia-susceptible mice display aberrations in intracellular calcium signaling and mitochondrial function.. Cell calcium, 93, 102325.
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6

Visualizing Candida biofilms using CLSM

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Candida spp. biofilms were stained using the Yeast Live-or-Dye Fixable Live/Dead staining kit, according to the manufacturer’s instructions (Biotium, Inc., Fremont, CA, USA). Briefly, samples were incubated with diluted dyes for 30 min at room temperature, then biofilms were air-dried for 15 min. Biofilms were visualized using a Nikon A1+ point confocal laser scanning microscope (CSLM) integrated with NIS-Elements C Software (Nikon Instruments), equipped with a Plan Apo λ 20× objective directly on the plastic support of the 96-well plate (λ exc = 489.3 nm and 561.4 nm).
Parolin C., Croatti V., Giordani B, & Vitali B. (2022). Vaginal Lactobacillus Impair Candida Dimorphic Switching and Biofilm Formation. Microorganisms, 10(10), 2091.
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7

Live Cell Imaging with Confocal Microscopy

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Live cell images were captured using an inverted microscope (Ti-E; Nikon, Tokyo, Japan) and a confocal laser microscope system (A1R; Nikon) equipped with an oil-immersion objective lens (Plan Apo VC 60×/1.40 NA Oil; Nikon). Cells were maintained in DMEM/F12 (1:1) (Life Technologies) supplemented with 10% FBS and warmed in a chamber set to 37°C (INUBG2H-TIZB; Tokai Hit) during observation. Z-stack images were oversampled by taking z-steps of 230 nm between acquired images. Images were captured and analyzed using NIS-Elements C software (Nikon).
Kuragano M., Uyeda T.Q., Kamijo K., Murakami Y, & Takahashi M. (2018). Different contributions of nonmuscle myosin IIA and IIB to the organization of stress fiber subtypes in fibroblasts. Molecular Biology of the Cell, 29(8), 911-922.
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8

Quantifying Expansion Factor Imaging

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To quantify the expansion factors (Figures 1A–C), the Nikon confocal AX setup was used. This modality allows the capture of images with a maximum field of view of 8,192 x 8,192 pixels to find corresponding regions before and after the expansion process. A Plan APO 60x/1.24 oil immersion objective was used to image the non-expanded sample and a Plan APO 60x/1.27 water immersion objective was used for the expanded samples. The microscope is equipped with an AX R Galvano scan head and a 2K resonant scanner. A DUX-VB detector unit with four GaAsP PMTs was used for confocal imaging. The AF488 fluorophore was excited using 12% of the 488 nm DPSS laser. The built-in dye preset of Nikon's NIS-Elements C software was used to select the appropriate excitation wavelength and filter settings for the specific dye.
Sachs S., Reinhard S., Eilts J., Sauer M, & Werner C. (2024). Visualizing the trans-synaptic arrangement of synaptic proteins by expansion microscopy. Frontiers in Cellular Neuroscience, 18, 1328726.
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9

Laser-Induced DNA Damage Kinetics

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Single strand breaks or DSBs of DNA were induced using a Nikon A1 laser microdissection system (Nikon). U2OS cells in four-well plates were transfected with indicated GFP-tagged expression vector for 48 h and then were incubated with 10 μM of 5-brome-2′-deoxyuridine for 24 h before laser-induced DSBs. Ten cells per well were subjected to laser-induced DSBs during 10 s using the × 60 oil objective. Fixed wavelength of ultraviolet A laser (405 nm) was used for laser microdissection in the temperature-controlled chamber with CO2 supplier. After laser treatment, cells were incubated at 37 °C for the indicated times. The intensity of each laser stripe in each time point was determined using confocal microscope. The kinetic analyses were performed using the NIS elements C software (Nikon). Each data series was normalized with respect to baseline values. Reconstitution assay has been carried out in U2OS cells transfected with TRAIP siRNA together with siRNA-resistant WT TRAIP, TRAIP-D2 or -D6 expression vector.
Soo Lee N., Jin Chung H., Kim H.J., Yun Lee S., Ji J.H., Seo Y., Hun Han S., Choi M., Yun M., Lee S.G., Myung K., Kim Y., Chul Kang H, & Kim H. (2016). TRAIP/RNF206 is required for recruitment of RAP80 to sites of DNA damage. Nature Communications, 7, 10463.
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10

Visualizing Extracellular Vesicles with Hoechst

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EV from cells infected with vF13-HA were purified from clarified supernatants as described above except the ethanol fixation step was omitted. Unfixed, purified EV were washed twice with 10mM Tris (pH 9.0) and resuspended 10mM Tris (pH 9.0) containing 10μg/ml Hoechst 34580 (AdipoGen). After 1h, EV were pelleted at 16,000 × g for 10 min, washed thrice with 10mM Tris (pH 9.0) and resuspended in 100 μl of 10mM Tris (pH 9.0). 30 μl of resuspended EV was spotted onto a #1.5 coverslip and incubated at room temperature for 1h. After which, coverslips were washed twice with 10mM Tris (pH 9.0) and mounted with ProLong Diamond (ThermoFisher). Mounted coverslips were imaged using a Nikon A1R HD laser scanning confocal microscope as a z-series. Collected z-stacks were deconvoluted using the NIS-Elements C software (Nikon) and maximum intensity projections were created using ImarisViewer software (Oxford Instruments).
Monticelli S.R., Bryk P., Brewer M.G., Aguilar H.C., Norbury C.C, & Ward B.M. (2021). An increase in glycoprotein concentration on extracellular virions dramatically alters vaccinia virus infectivity and pathogenesis without impacting immunogenicity. PLoS Pathogens, 17(12), e1010177.
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