Adi eks 700b

Manufactured by Enzo Life Sciences

Sourced in United States, Denmark

The ADI-EKS-700B is a laboratory instrument designed for the measurement of electrical conductivity. It features a digital display for accurate readings and can be used to test the conductivity of various solutions.

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Lab products found in correlation

Atp detection kit, by Beyotime (1 mentions) Hmgb1 elisa kit, by Arigo Biolaboratories (1 mentions) Adi eks 715, by Enzo Life Sciences (1 mentions)

5 protocols using adi eks 700b

HSF1 and HSP70 ELISA Assay

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HSF1 (ADI-900-199 from Enzo) and HSP70 (ADI-EKS-700B from Enzo) ELISAs were performed on 200 μg protein according to manufacturer’s instructions.

Kirkegaard T., Gray J., Priestman D.A., Wallom K.L., Atkins J., Olsen O.D., Klein A., Drndarski S., Petersen N.H., Ingemann L., Smith D.A., Morris L., Bornæs C., Jørgensen S.H., Williams I., Hinsby A., Arenz C., Begley D., Jäättelä M, & Platt F.M. (2016). Heat Shock Protein-based therapy as a potential candidate for treating sphingolipidoses. Science translational medicine, 8(355), 355ra118.

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Quantification of CRT, ATP, HMGB1, and HSP70/90

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CRT: Cells were collected with 0.25% trypsin without EDTA, washed twice with cold PBS, and incubated with Alexa Fluor 488 fluorescent anti-calreticulin antibodies in 100 μl of FACS buffer at a 1:100 dilution at 4 °C in darkness for 1 h. The expression of calreticulin (CRT) was analyzed by flow cytometry. ATP: The working fluid was configured according to the instructions of the ATP detection kit (#S0026, Biyuntian). HMGB1: According to the instructions of the high mobility group box-1 protein (HMGB1) ELISA kit (#ARG81351, Arigo), the supernatant of the cells was collected and assessed. HSP70/90: According to the instructions of the HSP70/90 ELISA kit (ADI-EKS-700B, ENZO), the supernatant of the cells to be tested was collected and assessed.

Yang X., Yang J., Gu X., Tao Y., Ji H., Miao X., Shen S, & Zang H. (2022). (−)-Guaiol triggers immunogenic cell death and inhibits tumor growth in non-small cell lung cancer. Molecular and Cellular Biochemistry, 478(7), 1611-1620.

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ELISA Protocol for Enzyme Assay

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Sample preparation and ELISA reaction were carried out according to the manufacturer's instructions (ADI-EKS-700B; Enzo Life Sciences).

Carnemolla A., Labbadia J.P., Lazell H., Neueder A., Moussaoui S, & Bates G.P. (2014). Contesting the dogma of an age-related heat shock response impairment: implications for cardiac-specific age-related disorders. Human Molecular Genetics, 23(14), 3641-3656.

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Quantifying HSP70 Levels in Red Blood Cells

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Among this group of proteins, we quantified levels of HSP70 because it is the most conserved protein in evolution and is very affected by multiple stressors (Daugaard et al., 2007; (link)Kregel, 2002) (link). Analyses of HSP70 were carried out at the Ecophysiology Laboratory at Estación Biológica de Doñana (Seville, Spain). HSP70 levels in red blood cells were quantified with a commercial ELISA kit (ADI-EKS-700B, ENZO Biochem Inc., Farmengdale, New York) following manufacturer instructions. Total proteins were measured using the Bradford method (Kruger, 1994) (link) and HSP70 values were corrected according to total protein concentration in the samples.

Ibáñez-Álamo J.D., Jimeno B., Gil D., Thomson R.L., Aguirre J.I., Díez-Fernández A., Faivre B., Tieleman B.I, & Figuerola J. (2020). Physiological stress does not increase with urbanization in European blackbirds: Evidence from hormonal, immunological and cellular indicators. The Science of the total environment, 721.

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Quantifying Tissue Distribution of rHSP70

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The concentration of rHSP70 in plasma and tissues was measured by ELISA (ADI-EKS-715 or ADI-EKS-700B, Enzo Life Sciences) and autoradiography using a gamma counter (Cobra II) for studies of I125-rHSP70.
The studies were performed in 54 outbred male mice from Taconic Europe A/S, Ejby, Denmark. All animal studies were approved by Dyreforsøgstilsynet (Denmark) and carried out in accordance with the National Institutes of Health guide for the Care and Use of Laboratory Animals guidelines. At start of the acclimatization period, the mice were 5 to 6 weeks old. An acclimatization period of at least 5 days was allowed in order to reject animals in poor condition or at the extremes of the weight ranges. The animals were dosed either I.V. or I.P. with 1-50 mg/kg [¹²⁵I]-rHSP70 as stated in the figure text. Blood sampling for toxicokinetic calculations was performed at the following time points: 5, 15, 30, 60, 120 and 360 min post-treatment. For distribution analysis, tissue samples were collected and saline perfused. Samples were measured using a gamma counter (Cobra II) as well as bioanalysis (ELISA) according to the manufacturers instructions.

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