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8 protocols using sodium nitrate

1

Synthesis and Characterization of Graphene

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Aniline (C6H2NH2, MW = 93.13, 99.5%), N,N-dimethylformamide (DMF) (linear formula HCON(CH3)2, >99.8%, molecular weight (MW): 73.09 g mol−1, d = ∼0.944 g ml−1 (lit.)), polystyrene (linear formula [CH2CH(C6H5)] n, average MW: ∼280.000 g mol−1, Tg = 100 °C, d = 1.047 g ml−1 at 25 °C), and graphite (empirical formula (Hill Notation) C, MW: 12.01 g mol−1, powder, <20 μm, synthetic, mp = 3652–3697 °C (lit.)) are purchased from Sigma Aldrich Co., Ltd, USA. Hydrochloric acid 37% (HCL, MW: 36.46, d = 1.18 g cm−3 (20 °C)), ammonium peroxodisulphate (APS) ((NH4) 2S2O8, MW: 228.2 g mol−1, d = 1.98 g cm−3 (20 °C)), sodium nitrate (NaNO3, MW: 84.99 g mol−1, d = 2.26 g cm−3 (20 °C)), and sulphuric acid 95% (H2SO4, MW: 98.08 g mol−1, d = 1.84 g cm−3 (20 °C)) are purchased VWR CHEMICALS Analwr Normapur. Potassium permanganate (KMnO4, >99.0%, d = 158.04 g mol−1) and hydrogen peroxide 30% (H2O2, 29.0% specific gravity) are purchased from BDH Laboratory Supplies Pools, BH15 TD, England. All the chemicals are used without further treatment.
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2

Comprehensive LC-MS Analytical Methodology

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LC-MS-grade acetonitrile and ammonium acetate, as well as NH3, were obtained from VWR (Vienna, Austria). Analytical-grade acarbose, arabinose, erythritol, fructose, galactose, glucose, inositol, lactitol, lactose, maltitol, raffinose, rhamnose, ribose, sucrose, xylitol, potassium chloride, ammonium iodide and potassium bromide were purchased from Sigma Aldrich (Schnelldorf, Germany). Erythrose, isomaltulose, lyxose, maltose, maltotriose, mannitol, mannose, sorbitol, sorbose, xylose, sodium nitrate and sodium sulfate were purchased from VWR (Vienna, Austria). LC-MS-grade water (< 0.055 µS cm−1) from an ultrapure water purification system (Sartorius, Göttingen, Germany) was used for both elution and sample preparation. Food samples produced by various companies were obtained from local supermarkets. Sample matrices have been selected over a wide range of beverages and food to investigate the impact on sample preparation and robustness of the analytical method. Food and beverages were stored at the recommended temperature until analysis.
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3

Optimization of Fungal Growth Media

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In the study, investigating different levels of carbohydrate and nitrogen source, the liquid media contained 50 g/L,100 g/L and 150 g/L of sucrose (MP biomedicals, Eschwege, Germany), and 4.6 g/L, 6.9 g/L and 9.2 g/L of ammonium tartrate (Acros organics, NJ, USA) or 3 g/L, 4.5 g/L and 6 g/l of sodium nitrate (VWR, Herlev, Denmark), and the rest of the media were composed as the base recipe. In all media, 250 mL were prepared using MQ H2O and autoclaved for 15 min at 121 °C after mixing. A capacity of 50 mL was then decanted into four Erlenmeyer flasks inoculated with 10,000 spores of OE::fsr6 and incubated at 25 °C at 100 rpm for seven days, constituting the quadruplicates of all experiments. The pH was adjusted to 5.6 ± 0.2 for all media.
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4

Characterization of Organic Acids and Compounds

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The following chemicals, including their sources, purity, and CAS numbers, were used: citric acid monohydrate, Fisher, ACS grade, 5949-29-1; ᴅʟ-malic acid, Alfa Aesar, 98%, 6915-15-7; ᴅʟ-isocitric acid, trisodium salt hydrate, Acros Organics, 95%, 1637-73-6; ᴅʟ-lactic acid, TCI, >85%, 50-21-5; ᴅʟ-glyceric acid, TCI, 20% in water (ca. 2 mol/L), 473-81-4; ᴅʟ-tartaric acid, TCI, >99%, 133-37-9; glutaric acid, Acros Organics, 99% 110-94-1; tricarballylic acid, Alfa Aesar, 98%, 99-14-9; adipic acid, Sigma, 99%, 124-04-9; acetic acid, Sigma, ACS grade, 64-19-7; pimelic acid, Alfa Aesar; >98%, 111-16-0; succinic acid, TCI, >99%, 110-15-6; tartronic acid, Sigma, >97%, 80-69-3; sodium mesoxalate monohydrate, Chemodex, >98%, 31635-99-1; ᴅʟ-2-hydroxybutyric acid sodium salt, Alfa Aesar, >97%, 5094-24-6; ᴅʟ-3-hydroxybutyric acid sodium salt, Chem Impex Int’l Inc., 100.3%, 150-83-4/306-31-0; ascorbic acid, TCI, >99%, 50-81-7; ammonium nitrate, Fisher, ACS grade, 6484-52-2; sodium nitrate, VWR, ACS grade, 7631-99-4; sodium hydroxide, VWR, ACS grade, 1310-73-2; nitric acid, Sigma, ACS grade, 7697-37-2; and sodium azide, Sigma, 99.8%, 26628-22-8. Lacey carbon, 300 mesh, copper grids (product #01895) from Ted Pella, Inc. were used for electron microscopy.
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5

Zirconium-based MOF for Arsenate Removal

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Unless otherwise stated, all the chemicals were used as received without further purification. The reagents including zirconium(IV) chloride (ZrCl4, 99.5%), 1,4-benzenedicarboxylic acid (BDC, 98%), and sodium arsenate dibasic heptahydrate (Na2HAsO4•7H2O, 98%) were purchased from Sigma-Aldrich. Moreover, ethanol (99.9%), dimethylformamide (DMF, 99.9%), sodium nitrate (99%), sodium chloride (99%), sodium sulfate, anhydrous (99%), sodium carbonate anhydrous (99.8%), nitric acid (68%), and sodium hydroxide (99%) were purchased from VWR. The stock solution of 1000 mg/L arsenate was obtained by dissolving Na2HAsO4•7H2O in 1 L deionized (DI) water (Analytic lab, ACEX, Imperial College London). The solutions of required concentrations used in this study were prepared by diluting the arsenate stock solution with DI water. pH adjustment was conducted using nitric acid or sodium hydroxide.
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6

Synthesis and Characterization of Polyisobutylene Derivatives

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Chemicals 2-Propanol (HPLC grade), methanol (HPLC grade), ethanol (HPLC grade), acetone (HPLC grade). and sodium nitrate were purchased from VWR International (Leuven, Belgium). Ammonium chloride and ammonium nitrate from Reanal (Budapest, Hungary) were used. Sodium chloride (ACS reagent), 2-butanol (ReagentPlus), n-hexanol (reagent grade), and n-octanol (ACS reagent) were received from Sigma-Aldrich (Taufkirchen, Germany). Dichloromethane (a.r. grade) was purchased from Molar Chemicals (Budapest, Hungary) and distillated before use.
The structures, the number-average molecular weights (M n ), and the polydispersities (M w /M n ) of the polyisobutylene derivatives determined by gel permeation chromatography (GPC) and mass spectrometry (MS) are compiled in Table 1.
All of the polyisobutylenes were synthesized by living carbocationic polymerization. The synthetic procedures for the PIB samples can be found in the Supporting Information.
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7

Evaluation of Antidiabetic and Antioxidant Potential

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STZ (Streptozotocin) and 3,5-Dinitrosalicylic acid (DNSA) (Sisco Research Laboratories Pvt. Ltd, India), α-amylase (Blulux Laboratories Pvt. Ltd., Faridaban, India), Acarbose (Bayer, Germany), Aluminum chloride, sodium carbonate, sodium nitrate, starch, sodium chloride, sodium hydroxide, potassium sodium tartrate tetrahydrate, disodium hydrogen phosphate and sodium dihydrogen phosphate (BDH Laboratory Supplies Ltd, England), 2,2-diphenyl-1-picrylhydrazine (DPPH), Galic acid, qurcetin and Folin-Ciocalteu's reagent (Sigm Chemicals Co, St. Louis, MO, USA), glibenclamide (Sanof–Aventis, USA) and glucose (Munchen, Germany).
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8

Zircon Raffinate Solution Processing

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The Egyptian Nuclear Materials Authority provides zircon raffinate solution. HCl acid, NaOH, HNO3 acid, and H2SO4 acid were purchased from Merck, Germany. Acrylic acid (AA) with 99% purity was obtained from Elf Chem. Co, France. Loba Chemie India provided 99% pure malic acid (MA). Methylene bis-acrylamide (DAM) and extra-pure graphite powders were provided by Merck, Germany. BDH Chemicals, Ltd., England, produced potassium permanganate (KMnO4) and sodium nitrate (NaNO3). Alpha Co., India, also delivered hydrogen peroxide, while ascorbic acid was obtained from Bratachem Co., Egypt.
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