Gill s hematoxylin

Manufactured by Agilent Technologies

Sourced in United States

Gill's hematoxylin is a laboratory stain used in histology and cytology. It is a regressive stain that selectively colors the nuclei of cells blue. Gill's hematoxylin is commonly used in the preparation and analysis of tissue samples for microscopic examination.

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Lab products found in correlation

Biotinylated secondary antibody, by Agilent Technologies (1 mentions) Peroxidase dab kit, by Agilent Technologies (1 mentions) Edta ph 9, by Agilent Technologies (1 mentions) Sa 5004, by Vector Laboratories (1 mentions) M o m immunodetection kit, by Vector Laboratories (1 mentions) Ba 1000, by Vector Laboratories (1 mentions) Bz x700 microscope, by Keyence (1 mentions) Glycergel, by Agilent Technologies (1 mentions) Horseradish peroxidase conjugated anti rabbit immunoglobulin polymer, by Immunologic (1 mentions)

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Hematoxylin (230 citations)

4 protocols using gill s hematoxylin

Immunocytochemical Analysis of GC Cell Binding

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Example 6

Immuocytochemistry (ICC) Analysis on Binding of MAb1738 with GC Cells

Fifty microliters of 1×10⁶GC cells was loaded into each cytospin chamber hole and were spun onto slides, followed by fixation with 4% paraformaldehyde/PBS solution, dehydration with 70% ethanol and then air drying. The slides were rehydrated in PBST in a flat position for 5 minutes and then incubated in 10% goat serum/PBS solution. The slides were incubated with MAb1738 or an irrelevant mAb (isotype control) for 1 hour at RT or overnight at 4° C., and then washed twice with PBST. The slides were then incubated with HRP-labeled goat anti-mouse IgG Fc-HRP (Jackson ImmunoResearch Laboratories) at 1:500 dilution for 30 minutes. Detection of mAb staining on cancer cells was performed with 0.125% aminoethylcarbazole chromogenic substrate for 5-10 minutes at RT, and the mAb stained cytospin slides were counterstained with Gill's hematoxylin (Dako, Carpinteria, Calif., USA).

FIG. 4 shows that MAb1738 mAb could bind to all three GC cell lines tested, including BGC823, MKN45 and GES-1. The target was exclusively located on the surface of the cells. In contrast, the irrelevant mAb did not stain the cells. This makes MAb1738 a good candidate in targeting various GC cells with increased expression levels of PODXL-v2 and/or PODXL-v2-Del.

US11267898B2. Anti-PODXL antibody MAI1738 and its use for cancer treatment (2022-03-08). MedAbome, Inc. [US]. Inventors: Mason Lu [US], Qinhong Ma [US], Mary Q. Xu [US], Jianyu Zhu [US], Yinghui Rong [US], Debashree Banerjee [US].

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Immunohistochemistry of Formalin-Fixed Tissues

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Immunohistochemistry was performed on 2 µm-thick sections obtained from formalin-fixed tissue embedded in paraffin. After dewaxing and rehydrating, heat-induced epitope retrieval was performed by boiling the slides with EDTA (pH 9) (Dako, Glostrup, Denmark). Endogenous peroxidase was blocked with 3% hydrogen peroxide followed by incubation with mouse-to-mouse blocking reagent to inhibit endogenous mouse immunoglobulin and then another blocking with blocked BSA 5%. Sections were incubated overnight at +4 °C with mouse monoclonal α-MF20 antibody (dilution 1:50; DSHB) or rabbit monoclonal α-SNAI2 (dilution 1:100). Detection of the primary antibody was performed by using the appropriate secondary biotinylated antibody (Dako, Carpinteria, USA) and the peroxidase DAB kit (Dako, Carpinteria, USA) with or without counterstaining with Gill’s hematoxylin (Dako, Carpinteria, USA). Negative controls were stained in parallel with either isotype non-specific IgG or only the primary antibody. The light microscopy imaging was performed on a Nikon E600 light microscope equipped with NIS Elements BR software, using 20x objective.

Pomella S., Sreenivas P., Gryder B.E., Wang L., Milewski D., Cassandri M., Baxi K., Hensch N.R., Carcarino E., Song Y., Chou H.C., Yohe M.E., Stanton B.Z., Amadio B., Caruana I., De Stefanis C., De Vito R., Locatelli F., Chen Y., Chen E.Y., Houghton P., Khan J., Rota R, & Ignatius M.S. (2021). Interaction between SNAI2 and MYOD enhances oncogenesis and suppresses differentiation in Fusion Negative Rhabdomyosarcoma. Nature Communications, 12, 192.

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Immunohistochemical Analysis of Tumor Samples

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Dissected tumors were fixed in 10% neutral-buffered formalin overnight, embedded in paraffin, and sectioned at 5-μm intervals. Hematoxylin counterstain was performed using standard procedures. All paraffin-embedded sections for immunohistochemistry were deparaffinized, heated in a microwave in 0.01 M sodium citrate buffer for antigen retrieval, treated with 3% H₂O₂ for 10 min, and rinsed in H₂O and PBS. For the anti-Ki67 antibody (1:1000; Cell Signaling Technology, #9027) immunohistochemistry, sections were blocked in 10% goat serum in PBS followed by primary antibody incubation overnight at 4°C. For mouse monoclonal MF20 (1:10; Developmental Studies Hybridoma Bank (DSHB), supernatant), the M.O.M Immunodetection Kit (Vector Laboratories, BMK-2202) was used followed by primary antibody incubation overnight at 4°C. Detection of the primary antibody was performed by using the appropriate secondary biotinylated antibody (Vector laboratories, BA-1000), streptavidin peroxidase (SA-5004), and 3,3′-diaminobenzidine substrate (Vector Laboratories, SK-4100), with or without counterstaining with Gill’s hematoxylin (Dako, Carpinteria, USA). Negative controls were stained in parallel with either isotype nonspecific immunoglobulin G or only the primary antibody. Slides were imaged using the Keyence BZ-X700 microscope, 10× or 20× objective.

Shah A.M., Guo L., Morales M.G., Jaichander P., Chen K., Huang H., Cano Hernandez K., Xu L., Bassel-Duby R., Olson E.N, & Liu N. (2023). TWIST2-mediated chromatin remodeling promotes fusion-negative rhabdomyosarcoma. Science Advances, 9(17), eade8184.

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Immunohistochemistry of DLC-1 in Atherosclerosis

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Immunohistochemistry for DLC-1 in Human Atherosclerotic Plaques Immunohistochemistry was performed on 7-mm-thick, formalin-fixed, paraffinembedded iliac artery sections from an organ donor (female, 41 years old, smoker). Sections were first de-paraffinized in xylene followed by rehydration. To perform antigen retrieval, the sections were boiled in citrate buffer, pH 6, for 10 min. Thereafter, the sections were washed in Tris-buffered saline (TBS) and incubated overnight at room temperature in a mix of two polyclonal rabbit antibodies against DLC-1 or against PECAM-1 in 0.1% BSA in TBS. Control sections were treated with 0.1% BSA in TBS. The following day, the slides were incubated for 1 hr with a horseradish peroxidase-conjugated anti-rabbit immunoglobulin polymer (Immunologic) diluted 1:1 with TBS followed by development with 3,3 0 -diaminobenzidine (DAB) (Immunologic). The sections were counterstained very briefly with Gill's hematoxylin and mounted with glycer gel (Dako). Samples were approved by the institutional review board of Academic Medical Center (AMC) (Amsterdam), and consent was given.

Schimmel L., van der Stoel M., Rianna C., van Stalborch A.M., de Ligt A., Hoogenboezem M., Tol S., van Rijssel J., Szulcek R., Bogaard H.J., Hofmann P., Boon R., Radmacher M., de Waard V., Huveneers S, & van Buul J.D. (2018). Stiffness-Induced Endothelial DLC-1 Expression Forces Leukocyte Spreading through Stabilization of the ICAM-1 Adhesome. Cell reports, 24(12).

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