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Theninfrared conjugated secondaries

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The infrared-conjugated secondaries are laboratory reagents designed for use in various immunoassay techniques. They function as detection probes, binding to and visualizing target proteins or molecules of interest. These secondary antibodies are conjugated with near-infrared fluorescent dyes, enabling sensitive and quantitative detection using specialized imaging equipment.

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Lab products found in correlation

Mini tgx gels, by Bio-Rad (2 mentions) Re blot plus strong solution, by Merck Group (2 mentions) Trans blot turbo, by Bio-Rad (2 mentions) Odyssey blocking buffer, by LI COR (2 mentions) Nupage 3 8 tris acetate gel, by Thermo Fisher Scientific (2 mentions) Protease phosphatase inhibitor, by Cell Signaling Technology (2 mentions) Laemmli sample buffer, by Bio-Rad (2 mentions) Nupage 4 12 bis tris gel, by Thermo Fisher Scientific (2 mentions)

2 protocols using theninfrared conjugated secondaries

1

Protein Extraction and Western Blot Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were lysed in RIPA buffer with protease/phosphatase inhibitor
(cat# 5872, Cell Signaling Technology, Danvers, MA) or Laemmli Sample Buffer
(cat# 1610747, Biorad, Hercules, CA) by sonication. PAGE was carried out
with manufacturer-recommended buffers on 4-20% or 7.5% Mini TGX Gels
(Biorad), NuPAGE 4-12% BisTris gels, or NuPAGE 3-8% Tris-Acetate gels
(Thermo). Semi-dry transfers were carried out for Biorad gels or NuPAGE
BisTris gels at 2.5A for 5-15 minutes using the Trans-Blot-Turbo (Biorad).
Wet transfers were carried out for Tris-Acetate gels at 30V overnight. All
blocking was performed with Odyssey Blocking Buffer (Licor). Primary
antibodies were incubated with membranes overnight at 4C, then
infrared-conjugated secondaries (Licor) were added 1:10,000 and imaged on a
Licor Odyssey Scanner. Quantifications were carried out using Image Studio
v4.0. Blots were stripped with Reblot Plus Strong Solution (Millipore
Sigma). A list of antibodies used can be found in the Supplementary Methods Key Reagents
table.
Ardeljan D., Steranka J.P., Liu C., Li Z., Taylor M.S., Payer L.M., Gorbounov M., Sarnecki J.S., Deshpande V., Hruban R.H., Boeke J.D., Fenyö D., Wu P.H., Smogorzewska A., Holland A.J, & Burns K.H. (2020). Cell fitness screens reveal a conflict between LINE-1 retrotransposition and DNA replication. Nature structural & molecular biology, 27(2), 168-178.
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2

Protein Extraction and Western Blot Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were lysed in RIPA buffer with protease/phosphatase inhibitor
(cat# 5872, Cell Signaling Technology, Danvers, MA) or Laemmli Sample Buffer
(cat# 1610747, Biorad, Hercules, CA) by sonication. PAGE was carried out
with manufacturer-recommended buffers on 4-20% or 7.5% Mini TGX Gels
(Biorad), NuPAGE 4-12% BisTris gels, or NuPAGE 3-8% Tris-Acetate gels
(Thermo). Semi-dry transfers were carried out for Biorad gels or NuPAGE
BisTris gels at 2.5A for 5-15 minutes using the Trans-Blot-Turbo (Biorad).
Wet transfers were carried out for Tris-Acetate gels at 30V overnight. All
blocking was performed with Odyssey Blocking Buffer (Licor). Primary
antibodies were incubated with membranes overnight at 4C, then
infrared-conjugated secondaries (Licor) were added 1:10,000 and imaged on a
Licor Odyssey Scanner. Quantifications were carried out using Image Studio
v4.0. Blots were stripped with Reblot Plus Strong Solution (Millipore
Sigma). A list of antibodies used can be found in the Supplementary Methods Key Reagents
table.
Ardeljan D., Steranka J.P., Liu C., Li Z., Taylor M.S., Payer L.M., Gorbounov M., Sarnecki J.S., Deshpande V., Hruban R.H., Boeke J.D., Fenyö D., Wu P.H., Smogorzewska A., Holland A.J, & Burns K.H. (2020). Cell fitness screens reveal a conflict between LINE-1 retrotransposition and DNA replication. Nature structural & molecular biology, 27(2), 168-178.
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