Cell light edu apollo567 in vitro kit 100t

Cell-Light EdU Apollo567 In Vitro Kit (100T) (C10310-1) was provided by Guangzhou RiboBio Co., Ltd. (Guangzhou, Guangdong, China). This kit was performed to detect cell proliferation. Firstly, HUVECs (4x10³ cells per well) were seeded onto 96-well plates. After that, the cells were cultured with 50 μM EdU for 2 h followed by washed using PBS. Then, cells were incubated with 100 μL PBS containing 4% paraformaldehyde for 30 min. After that, cells were incubated with 100 μL 1 mg/mL DAPI for 10 min at room temperature in the darkness. Finally, a fluorescence microscope was used to observe the results of staining.

The biocompatibility of PEP@GO and the impact of the above experimental treatments on HUVEC cell proliferation were assessed using EDU uptake assays. HUVECs were placed in 24-well plates and treated with appropriate sEVs or miRNA formulations for 24 h. Following that, a Cell-Light EDU Apollo567 In vitro Kit (100 T) (RiboBio Co., Ltd., China) was utilized according to the instructions.

Near-confluent C2C12 cells (80–90%) were transfected with a vector overexpressing 2310043L19Rik (lnc-231), a control vector (pcDNA3.1), silnc-231, or NC silnc-231 (double strand). And the co-transfection group, transfected with pcDNA3.1+ NC mimic, miR-125a-5p, lnc-231+ miR-125a-5p, and lnc-231, respectively. After 12 h of transfection, C2C12 cells were cultured in fresh GM that contained 5-ethynyl-29-deoxyuridine (EdU; final concentration, 10 mM) for 4 h. An EdU assay with the Cell-Light EdU Apollo567 In Vitro Kit 100T (RIBOBIO, Guangzhou, China) was performed to test the proliferation of C2C12 cells and analyzed by confocal microscopy (LSM 700 META; Zeiss, Jena, Germany) to detect the EdU stained cells.