Isopropyl alcohol

Nannochloropsis gaditana dry biomass was provided by Algaenergy S.A. (Alcobendas, Spain).
Chloroform and isopropyl alcohol were purchased from Scharlab S.L. (Sentmenat, Spain). Methanol was purchased from Lab Scan Analytical Sciences (Gliwice, Poland). Hexane and HPLC-grade solvents (2,2,4-trimethyl pentane, methyl tert-butyl ether (MTBE)) were purchased from Macron Fine Chemicals (Gliwice, Poland). Absolute ethanol (PRS grade), sodium hydrogen carbonate, and potassium hydroxide were purchased from Panreac Química S.A (Barcelona, Spain). The water used was Milli-Q grade (Millipore Sigma, Burlington, MA, USA). Viscozyme^® from Aspergillus aculeatus containing a wide range of carbohydrases, including arabinase, cellulase, beta-glucanase, hemicellulase, and xylanase, and Celluclast^® containing cellulase from Trichoderma reesei and Alcalase^® were kindly donated by Novozymes (Bagsvaerd, Denmark). Glyceryl trilinoleate, dioleoylglycerol (mixture of 1,3- and 1,2-isomers), 1-oleoyl-rac-glycerol, oleic acid, and ethyl linoleate used as HPLC standards was purchased from Sigma-Aldrich (St. Louis, MO, USA). All other reagents and solvents used were of analytical or HPLC grade.

Commercial dry powder biomass from microalga Isochrysis galbana was purchased from Neoalgae Micro Seaweed Products (Gijón, Spain). Chloroform and isopropyl alcohol were purchased from Scharlab S.L. (Sentmenat, Spain) Methanol was purchased from Lab Scan Analytical Sciences (Gliwice, Poland). Hexane and the solvents (2,2,4-trimethyl pentane, methyl tert-butyl ether (MTBE)) used for HPLC analyses were HPLC-grade and purchased from Macron Fine Chemicals (Gliwice, Poland). Absolute ethanol (PRS grade), sodium hydrogen carbonate and potassium hydroxide were purchased from Panreac Quimica S.A (Barcelona, Spain). The water used was Milli-Q grade (Millipore Sigma, Burlington, MA , USA). Viscozyme^®® from Aspergillus aculeatus containing a wide range of carbohydrases, including arabinase, cellulase, beta-glucanase, hemicellulase and xylanase, and Celluclast^®® containing cellulase from Trichoderma reesei were kindly donated by Novozymes (Bagsvaerd, Denmark). Fatty acid methyl esters standard (Supelco 37 FAME Mix) was from Supelco (Bellefonte, PA, USA). Glyceryl trilinoleate, dioleoylglycerol (mixture of 1,3- and 1,2-isomers), 1-oleoyl-rac-glycerol, oleic acid and ethyl linoleate used as HPLC standards was purchased from Sigma-Aldrich (St. Louis, MO, USA). All other reagents and solvents used were of analytical or HPLC grade.