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Stat3 inhibitor s3i 201

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STAT3 inhibitor S3I-201 is a small molecule that targets the STAT3 (Signal Transducer and Activator of Transcription 3) protein. STAT3 is a transcription factor involved in various cellular processes. S3I-201 functions as an inhibitor of STAT3 activity.

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Lab products found in correlation

Alexa fluor 647 anti rabbit igg h l, by Jackson ImmunoResearch (2 mentions) Alexa fluor 647 anti phospho stat3 tyr705 clone 4 p stat3, by BD (2 mentions) Alexa fluor 647 anti phospho stat5 tyr694 clone 47 stat5, by BD (2 mentions) Alexa fluor 647 anti phospho p44 42 mapk erk1 2 thr202 tyr204 clone 20a, by BD (2 mentions) Anti phospho akt thr308 clone d25e6, by Cell Signaling Technology (2 mentions) Pimozide, by Cayman Chemical (2 mentions) Recombinant human ccl2, by BioLegend (1 mentions)

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STAT3 inhibitor (5 citations)

3 protocols using stat3 inhibitor s3i 201

1

Phosphorylation Profiling of CAR-T Cells

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Phosphorylated proteins in the CAR-T cells were analyzed by intracellular flow cytometry following coculture with NALM-6 or K562 at an E:T ratio of 1:1. The cells were fixed with 1.6 % formaldehyde, followed by permeabilization by ice-cold methanol. The following antibodies were used: Alexa Fluor 647-anti-phospho-STAT3 (Tyr705) (clone 4/P-Stat3, BD Biosciences), Alexa Fluor 647-anti-phospho-STAT5 (Tyr694) (clone 47/Stat5, BD Biosciences), Alexa Fluor 647-anti-phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (clone 20A, BD Biosciences), and anti-phospho-Akt (Thr308) (clone D25E6, Cell Signaling Technology). Alexa Fluor 647-anti-rabbit IgG (H+L) (Jackson ImmunoResearch) was used as the secondary antibody following the staining with the anti–phospho-Akt antibody. The STAT3 inhibitor S3I-201 (Selleck Chemicals) and the STAT5 inhibitor pimozide (Cayman Chemical) were used at concentrations of 25 μM and 5 μM, respectively.
Kagoya Y., Tanaka S., Guo T., Anczurowski M., Wang C.H., Saso K., Butler M.O., Minden M.D, & Hirano N. (2018). A novel chimeric antigen receptor containing a JAK-STAT signaling domain mediates superior antitumor effects. Nature medicine, 24(3), 352-359.
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2

Inhibiting Monocyte Random Migration

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Isolated human CD14+CD16− monocytes were incubated with various inhibitors upon seeding. Several inhibitors were used to interfere with the random migration of monocytes: 20 μM RhoA Kinase ROCK inhibitor Y-27632 (Selleckchem), 100 μM Arp inhibitor CK666 (Selleckchem), 100 μM STAT3 inhibitor S3I-201 (Selleckchem), 10 μM Blebbistatin for non-muscle myosin II inhibition (Millipore Sigma), and 10 μM EIPA (Millipore Sigma) to block the sodium-hydrogen exchanger (NHE). Monocytes were incubated with 2μg/mL Pertussis toxin (EMD Millipore Crop), 20 μM CID-1067700 (MedChemExpress), and 2 μg/mL recombinant human CCL2 (Biolegend).
Wirtz D., Du W., Zhu J., Wu Y., Kiemen A., Wan Z., Hanna E, & Sun S. (2023). Mechano-induced homotypic patterned domain formation by monocytes. Research Square.
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3

Phosphorylation Profiling of CAR-T Cells

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Phosphorylated proteins in the CAR-T cells were analyzed by intracellular flow cytometry following coculture with NALM-6 or K562 at an E:T ratio of 1:1. The cells were fixed with 1.6 % formaldehyde, followed by permeabilization by ice-cold methanol. The following antibodies were used: Alexa Fluor 647-anti-phospho-STAT3 (Tyr705) (clone 4/P-Stat3, BD Biosciences), Alexa Fluor 647-anti-phospho-STAT5 (Tyr694) (clone 47/Stat5, BD Biosciences), Alexa Fluor 647-anti-phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (clone 20A, BD Biosciences), and anti-phospho-Akt (Thr308) (clone D25E6, Cell Signaling Technology). Alexa Fluor 647-anti-rabbit IgG (H+L) (Jackson ImmunoResearch) was used as the secondary antibody following the staining with the anti–phospho-Akt antibody. The STAT3 inhibitor S3I-201 (Selleck Chemicals) and the STAT5 inhibitor pimozide (Cayman Chemical) were used at concentrations of 25 μM and 5 μM, respectively.
Kagoya Y., Tanaka S., Guo T., Anczurowski M., Wang C.H., Saso K., Butler M.O., Minden M.D, & Hirano N. (2018). A novel chimeric antigen receptor containing a JAK-STAT signaling domain mediates superior antitumor effects. Nature medicine, 24(3), 352-359.
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