Laminin poly l ornithine coated 96 well plates

Manufactured by Corning

Laminin-poly-l-ornithine coated 96-well plates are a type of lab equipment designed for cell culture applications. The plates feature a surface coating that promotes cell adhesion and growth. The core function of these plates is to provide a suitable substrate for culturing cells in a 96-well format.

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Lab products found in correlation

Alamar blue, by Thermo Fisher Scientific (2 mentions) Spectramax spectrophotometer, by Thermo Fisher Scientific (2 mentions) Rgfp966, by Selleck Chemicals (1 mentions)

Spelling variants of this product

96-well plates (3030 citations) Laminin (77 citations) 96 wells (10 citations) Laminin-coated 96-well plates (3 citations) Poly-L-Ornithine (3 citations) Laminin-coated plates (2 citations)

2 protocols using laminin poly l ornithine coated 96 well plates

Compound Cytotoxicity Screening in Cells

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Cells were plated at 4000 cells/well in NS media into laminin-poly-l-ornithine coated 96-well plates (Corning). The compounds MI-3, RGFP-966, and AZD6102 (Selleckchem) were tested at different concentrations (range: 2 μM to 8 nM in serial dilutions) with six technical replicates per dose, and a DMSO control. Cell viability was assessed on day 7. Alamar blue (Thermo Fisher Scientific, Cat# DAL1025) was added and cells were incubated at 37 °C in the dark for 4 h. Fluorescence was measured on the Spectramax spectrophotometer and was normalized to the DMSO control.

Nikolic A., Maule F., Bobyn A., Ellestad K., Paik S., Marhon S.A., Mehdipour P., Lun X., Chen H.M., Mallard C., Hay A.J., Johnston M.J., Gafuik C.J., Zemp F.J., Shen Y., Ninkovic N., Osz K., Labit E., Berger N.D., Brownsey D.K., Kelly J.J., Biernaskie J., Dirks P.B., Derksen D.J., Jones S.J., Senger D.L., Chan J.A., Mahoney D.J., De Carvalho D.D, & Gallo M. (2023). macroH2A2 antagonizes epigenetic programs of stemness in glioblastoma. Nature Communications, 14, 3062.

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Cytotoxicity Assessment of ADC m276-PBD

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Cells were plated at 3000 cells/well in NS media into laminin–poly-L-ornithine-coated 96-well-plates (Corning). The antibody-drug conjugate, m276-PBD, was tested at different concentrations (40 nM–1.5 nM range) (see Supplemental Fig. S6B) in PBS. Each concentration was applied to six wells for each culture; PBS-only was used as a control. Cell viability was assessed on day 4. alamarBlue (Thermo Fisher Scientific, Cat# DAL1025) was added and incubated at 37°C in the dark for 4 h. Fluorescence was measured on the Spectramax spectrophotometer and normalized to the PBS control.

Johnston M.J., Nikolic A., Ninkovic N., Guilhamon P., Cavalli F.M., Seaman S., Zemp F.J., Lee J., Abdelkareem A., Ellestad K., Murison A., Kushida M.M., Coutinho F.J., Ma Y., Mungall A.J., Moore R., Marra M.A., Taylor M.D., Dirks P.B., Pugh T.J., Morrissy S., St Croix B., Mahoney D.J., Lupien M, & Gallo M. (2019). High-resolution structural genomics reveals new therapeutic vulnerabilities in glioblastoma. Genome Research, 29(8), 1211-1222.

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