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Cd34 human cord blood

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CD34+ human cord blood is a type of lab equipment that contains hematopoietic stem cells derived from human umbilical cord blood. These cells express the CD34 surface antigen, which is a marker for immature, undifferentiated cells. The core function of this product is to serve as a source of primary cells for research and development purposes.

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Lab products found in correlation

Stemspan sfem media, by STEMCELL (2 mentions) Human tpo, by ProSpec (2 mentions) Knockout serum replacement (ksr), by Thermo Fisher Scientific (2 mentions) Pen strep, by ProSpec (2 mentions)

2 protocols using cd34 human cord blood

1

Ex Vivo Expansion of Human HSCs

Check if the same lab product or an alternative is used in the 5 most similar protocols
CD34+ human cord blood (Lonza) was obtained according to an institutional review board-approved protocol. 13,600 CD34+ cells were co-cultured with murine rMSCs in StemSpan SFEM media (STEMCELL Technologies) supplemented with 10 % KnockOut Serum Replacement (Thermo Fisher Scientific), Pen/Strep, 10ng/ml of human TPO (PROSPEC). After co-culture for 6 days, the number of phenotypic Lin−CD34+CD38−CD90+CD49f+ human HSCs was assessed by FACS analysis.
Nakahara F., Borger D.K., Wei Q., Pinho S., Maryanovich M., Zahalka A.H., Suzuki M., Cruz C.D., Wang Z., Xu C., Boulais P.E., Ma'ayan A., Greally J.M, & Frenette P.S. (2019). Engineering a haematopoietic stem cell niche by revitalizing mesenchymal stromal cells. Nature cell biology, 21(5), 560-567.
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2

Ex Vivo Expansion of Human HSCs

Check if the same lab product or an alternative is used in the 5 most similar protocols
CD34+ human cord blood (Lonza) was obtained according to an institutional review board-approved protocol. 13,600 CD34+ cells were co-cultured with murine rMSCs in StemSpan SFEM media (STEMCELL Technologies) supplemented with 10 % KnockOut Serum Replacement (Thermo Fisher Scientific), Pen/Strep, 10ng/ml of human TPO (PROSPEC). After co-culture for 6 days, the number of phenotypic Lin−CD34+CD38−CD90+CD49f+ human HSCs was assessed by FACS analysis.
Nakahara F., Borger D.K., Wei Q., Pinho S., Maryanovich M., Zahalka A.H., Suzuki M., Cruz C.D., Wang Z., Xu C., Boulais P.E., Ma'ayan A., Greally J.M, & Frenette P.S. (2019). Engineering a haematopoietic stem cell niche by revitalizing mesenchymal stromal cells. Nature cell biology, 21(5), 560-567.
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