Lead chromate pbcro4

DMEM and Ham’s F12 50:50 mixture and GlutaGRO (L-alanyl-L-glutamine solution) were purchaed from Mediatech Inc (Herndon, VA). Cosmic Calf Serum was purchased from Hyclone (Logan, UT). All plasticware was purchased from BD Biosciences (Franklin Lakes, NJ). Dulbecco’s phosphate buffered saline (PBS), Gurr’s buffer, penicillin/streptomycin, sodium pyruvate and trypsin/EDTA were purchased from Life Technologies (Grand Island, NY). Crystal violet, calcium chloride, demecolchicine, lead chromate (PbCrO₄) and potassium chloride were purchased from Sigma-Aldrich (St. Louis, MO). Acetic acid and methanol were purchased from J.T. Baker (Phillipsburg, NJ). Giemsa stain was purchased from Biomedical Specialties Inc. (Santa Monica, CA). Titanium dioxide nanoparticles, Aeroxide TiO₂ P25, were purchased from Nippon Aerosil Co, LTD (Tokyo, Japan). Microscope slides and cytoseal 60 slide mounting medium were purchased from Thermo Fisher Scientific Inc. (Waltham, MA). Ethyl alcohol, Embed-812 kit, flat embedding mold, gluteraldehyde, osmium tetra oxide, sodium cacodylate, sodium maleate and uranyl acetate were purchased from EMS (Hatfield, PA).